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SLC38A2 promotes cell proliferation and invasion by promoting glutamine metabolism in adenomyosis

  • Authors:
    • Kui Wu
    • Wei Gong
    • Jia-Chang Hu
    • Ying-Chun Duan
    • Hui-Hui Ke
    • Li Chen
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, Shanghai 201399, P.R. China
    Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 218
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    Published online on: March 21, 2024
       https://doi.org/10.3892/etm.2024.12506
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Abstract

Adenomyosis is a benign uterine disorder that is associated with female infertility, a reduced clinical pregnancy rate and a high risk of miscarriage. Solute carrier family 38 member a2 (SLC38A2) is a glutamine (Gln) transporter that serves roles in various medical conditions. The present study aimed to reveal the role of SLC38A2 in adenomyosis. The mRNA expression levels of SLC38A2 in eutopic endometrial (EU) and ectopic endometrial (EC) tissues from adenomyotic patients were examined by reverse transcription‑quantitative PCR. EU and EC cell proliferation and invasion were analyzed by Cell Counting Kit‑8 and Transwell assays. Changes in the oxygen consumption rate (OCR) were determined to indicate the mitochondrial respiratory function and observed using a Seahorse analyzer. SLC38A2 expression in EC tissues was upregulated compared with that in normal endometrial tissues. SLC38A2 knockdown repressed EC cell proliferation and invasion. In addition, the Gln content and OCR were decreased in EC cells transfected with SLC38A2‑knockdown lentivirus, whereas SLC38A2 overexpression had the opposite effect in EU cells. Furthermore, the increased proliferation and invasion rates and Gln level induced by SLC38A2 overexpression in EU cells were alleviated by CB‑839, a glutaminase inhibitor. SLC38A2 overexpression promoted Gln metabolism and oxygen consumption rate, resulting in an increase in cell proliferation and invasion in the adenomyosis context. The present study indicated that reduction of SLC38A2 expression could be a novel target for adenomyosis therapy, and SLC38A2 may be a valuable clinical diagnostic molecule for adenomyosis.
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Copy and paste a formatted citation
Spandidos Publications style
Wu K, Gong W, Hu J, Duan Y, Ke H and Chen L: SLC38A2 promotes cell proliferation and invasion by promoting glutamine metabolism in adenomyosis. Exp Ther Med 27: 218, 2024.
APA
Wu, K., Gong, W., Hu, J., Duan, Y., Ke, H., & Chen, L. (2024). SLC38A2 promotes cell proliferation and invasion by promoting glutamine metabolism in adenomyosis. Experimental and Therapeutic Medicine, 27, 218. https://doi.org/10.3892/etm.2024.12506
MLA
Wu, K., Gong, W., Hu, J., Duan, Y., Ke, H., Chen, L."SLC38A2 promotes cell proliferation and invasion by promoting glutamine metabolism in adenomyosis". Experimental and Therapeutic Medicine 27.5 (2024): 218.
Chicago
Wu, K., Gong, W., Hu, J., Duan, Y., Ke, H., Chen, L."SLC38A2 promotes cell proliferation and invasion by promoting glutamine metabolism in adenomyosis". Experimental and Therapeutic Medicine 27, no. 5 (2024): 218. https://doi.org/10.3892/etm.2024.12506
Copy and paste a formatted citation
x
Spandidos Publications style
Wu K, Gong W, Hu J, Duan Y, Ke H and Chen L: SLC38A2 promotes cell proliferation and invasion by promoting glutamine metabolism in adenomyosis. Exp Ther Med 27: 218, 2024.
APA
Wu, K., Gong, W., Hu, J., Duan, Y., Ke, H., & Chen, L. (2024). SLC38A2 promotes cell proliferation and invasion by promoting glutamine metabolism in adenomyosis. Experimental and Therapeutic Medicine, 27, 218. https://doi.org/10.3892/etm.2024.12506
MLA
Wu, K., Gong, W., Hu, J., Duan, Y., Ke, H., Chen, L."SLC38A2 promotes cell proliferation and invasion by promoting glutamine metabolism in adenomyosis". Experimental and Therapeutic Medicine 27.5 (2024): 218.
Chicago
Wu, K., Gong, W., Hu, J., Duan, Y., Ke, H., Chen, L."SLC38A2 promotes cell proliferation and invasion by promoting glutamine metabolism in adenomyosis". Experimental and Therapeutic Medicine 27, no. 5 (2024): 218. https://doi.org/10.3892/etm.2024.12506
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