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Bulk and single cells transcriptomes with experimental validation identify USP18 as a novel glioma prognosis and proliferation indicator

  • Authors:
    • Yang Chen
    • Ren Li
    • Ziao Li
    • Biao Yang
    • Jianhang He
    • Jiayu Li
    • Peize Li
    • Zihan Zhou
    • Yongqiang Wu
    • Yuanli Zhao
    • Geng Guo
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, First Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China, School of Public Health, Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China, Department of Emergency, First Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China, Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100070, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 229
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    Published online on: March 26, 2024
       https://doi.org/10.3892/etm.2024.12517
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Abstract

The mechanism by which ubiquitin‑specific protease 18 (USP18) (enzyme commission: 3.4.19.12) inhibition in cancer promotes cell pyroptosis via the induction of interferon (IFN)‑stimulated genes has been recently demonstrated. It is also known that USP18 influences the epithelial‑mesenchymal transition of glioma cells. In the present study, the upregulation of USP18 in glioma was revealed through bulk transcriptome analysis, which was associated with poor prognosis in patients with glioma. Furthermore, USP18 levels affected the response to immunotherapy in patients with glioma. Single‑cell transcriptome and enrichment analyses demonstrated that USP18 was associated with type 1 IFN responses in glioma T cells. To demonstrate the effect of USP18 expression levels on glioma cells, USP18 expression was knocked down in U251 and U87MG ATCC cell lines. A subsequent Cell Counting Kit‑8 assay revealed that glioma cell viability was significantly decreased 4 days after USP18 knockdown. In addition, the knockdown of USP18 expression significantly inhibited the clonogenicity of U251 and U87MG ATCC cells. In conclusion, the present study demonstrated that knockdown of USP18 expression inhibited the proliferation of glioma cells, which may be mediated by the effect of USP18 on the IFN‑I response.
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Copy and paste a formatted citation
Spandidos Publications style
Chen Y, Li R, Li Z, Yang B, He J, Li J, Li P, Zhou Z, Wu Y, Zhao Y, Zhao Y, et al: Bulk and single cells transcriptomes with experimental validation identify USP18 as a novel glioma prognosis and proliferation indicator. Exp Ther Med 27: 229, 2024.
APA
Chen, Y., Li, R., Li, Z., Yang, B., He, J., Li, J. ... Guo, G. (2024). Bulk and single cells transcriptomes with experimental validation identify USP18 as a novel glioma prognosis and proliferation indicator. Experimental and Therapeutic Medicine, 27, 229. https://doi.org/10.3892/etm.2024.12517
MLA
Chen, Y., Li, R., Li, Z., Yang, B., He, J., Li, J., Li, P., Zhou, Z., Wu, Y., Zhao, Y., Guo, G."Bulk and single cells transcriptomes with experimental validation identify USP18 as a novel glioma prognosis and proliferation indicator". Experimental and Therapeutic Medicine 27.5 (2024): 229.
Chicago
Chen, Y., Li, R., Li, Z., Yang, B., He, J., Li, J., Li, P., Zhou, Z., Wu, Y., Zhao, Y., Guo, G."Bulk and single cells transcriptomes with experimental validation identify USP18 as a novel glioma prognosis and proliferation indicator". Experimental and Therapeutic Medicine 27, no. 5 (2024): 229. https://doi.org/10.3892/etm.2024.12517
Copy and paste a formatted citation
x
Spandidos Publications style
Chen Y, Li R, Li Z, Yang B, He J, Li J, Li P, Zhou Z, Wu Y, Zhao Y, Zhao Y, et al: Bulk and single cells transcriptomes with experimental validation identify USP18 as a novel glioma prognosis and proliferation indicator. Exp Ther Med 27: 229, 2024.
APA
Chen, Y., Li, R., Li, Z., Yang, B., He, J., Li, J. ... Guo, G. (2024). Bulk and single cells transcriptomes with experimental validation identify USP18 as a novel glioma prognosis and proliferation indicator. Experimental and Therapeutic Medicine, 27, 229. https://doi.org/10.3892/etm.2024.12517
MLA
Chen, Y., Li, R., Li, Z., Yang, B., He, J., Li, J., Li, P., Zhou, Z., Wu, Y., Zhao, Y., Guo, G."Bulk and single cells transcriptomes with experimental validation identify USP18 as a novel glioma prognosis and proliferation indicator". Experimental and Therapeutic Medicine 27.5 (2024): 229.
Chicago
Chen, Y., Li, R., Li, Z., Yang, B., He, J., Li, J., Li, P., Zhou, Z., Wu, Y., Zhao, Y., Guo, G."Bulk and single cells transcriptomes with experimental validation identify USP18 as a novel glioma prognosis and proliferation indicator". Experimental and Therapeutic Medicine 27, no. 5 (2024): 229. https://doi.org/10.3892/etm.2024.12517
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