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Anti‑obesity and immunostimulatory activity of Chrysosplenium flagelliferum in mouse preadipocytes 3T3‑L1 cells and mouse macrophage RAW264.7 cells 

  • Authors:
    • Jeong Won Choi
    • Gwang Hun Park
    • Hyeok Jin Choi
    • Jae Won Lee
    • Hae-Yun Kwon
    • Min Yeong Choi
    • Jin Boo Jeong
  • View Affiliations / Copyright

    Affiliations: Department of Forest Science, Andong National University, Andong, Gyeongsangbuk 36729, Republic of Korea, Forest Medicinal Resources Research Center, National Institute of Forest Science, Yeongju, Gyeongsangbuk 36040, Republic of Korea
    Copyright: © Choi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 315
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    Published online on: June 11, 2024
       https://doi.org/10.3892/etm.2024.12604
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Abstract

Chrysosplenium flagelliferum (CF) is known for its anti‑inflammatory, antioxidant and antibacterial activities. However, there is a lack of research on its other pharmacological properties. In the present study, the bifunctional roles of CF in 3T3‑L1 and RAW264.7 cells were investigated, focusing on its anti‑obesity and immunostimulatory effects. In 3T3‑L1 cells, CF effectively mitigated the accumulation of lipid droplets and triacylglycerol. Additionally, CF downregulated the peroxisome proliferator‑activated receptor (PPAR)‑γ and CCAAT/enhancer‑binding protein α protein levels; however, this effect was impeded by the knockdown of β‑catenin using β‑catenin‑specific small interfering RNA. Consequently, CF‑mediated inhibition of lipid accumulation was also decreased. CF increased the protein levels of adipose triglyceride lipase and phosphorylated hormone‑sensitive lipase, while decreasing those of perilipin‑1. Moreover, CF elevated the protein levels of phosphorylated AMP‑activated protein kinase and PPARγ coactivator 1‑α. In RAW264.7 cells, CF enhanced the production of pro‑inflammatory mediators, such as nitric oxide (NO), inducible NO synthase, interleukin (IL)‑1β, IL‑6 and tumor necrosis factor‑α, and increased their phagocytic capacities. Inhibition of Toll‑like receptor (TLR)‑4 significantly reduced the effects of CF on the production of pro‑inflammatory mediators and phagocytosis, indicating its crucial role in facilitating these effects. CF‑induced increase in the production of pro‑inflammatory mediators was controlled by the activation of c‑Jun N‑terminal kinase (JNK) and nuclear factor (NF)‑κB pathways, and TLR4 inhibition attenuated the phosphorylation of these kinases. The results of the pesent study suggested that CF inhibits lipid accumulation by suppressing adipogenesis and inducing lipolysis and thermogenesis in 3T3‑L1 cells, while stimulating macrophage activation via the activation of JNK and NF‑κB signaling pathways mediated by TLR4 in RAW264.7 cells. Therefore, CF simultaneously exerts both anti‑obesity and immunostimulatory effects.
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1 

Jin H, Han H, Song G, Oh HJ and Lee BY: Anti-obesity effects of GABA in C57BL/6J mice with high-fat diet-induced obesity and 3T3-L1 adipocytes. Int J Mol Sci. 25(995)2024.PubMed/NCBI View Article : Google Scholar

2 

Kang SA and Yu HS: Anti-obesity effects by parasitic nematode (Trichinella spiralis) total lysates. Front Cell Infect Microbiol. 13(1285584)2024.PubMed/NCBI View Article : Google Scholar

3 

Shi Q, Wang Y, Hao Q, Vandvik PO, Guyatt G, Li J, Chen Z, Xu S, Shen Y, Ge L, et al: Pharmacotherapy for adults with overweight and obesity: A systematic review and network meta-analysis of randomised controlled trials. Lancet. 399:259–269. 2022.PubMed/NCBI View Article : Google Scholar

4 

Huttunen R and Syrjänen J: Obesity and the outcome of infection. Lancet Infect Dis. 10:442–443. 2010.PubMed/NCBI View Article : Google Scholar

5 

Muscogiuri G, Pugliese G, Laudisio D, Castellucci B, Barrea L, Savastano S and Colao A: The impact of obesity on immune response to infection: Plausible mechanisms and outcomes. Obes Rev. 22(e13216)2021.PubMed/NCBI View Article : Google Scholar

6 

Nour TY and Altintaş KH: Effect of the COVID-19 pandemic on obesity and its risk factors: A systematic review. BMC Public Health. 23(1018)2023.PubMed/NCBI View Article : Google Scholar

7 

Tak YJ and Lee SY: Anti-obesity drugs: Long-term efficacy and safety: An updated review. World J Mens Health. 39:208–221. 2021.PubMed/NCBI View Article : Google Scholar

8 

Sun NN, Wu TY and Chau CF: Natural dietary and herbal products in anti-obesity treatment. Molecules. 21(1351)2016.PubMed/NCBI View Article : Google Scholar

9 

Yan WJ, Yang TG, Liao R, Wu ZH, Qin R and Liu H: Complete chloroplast genome sequence of Chrysosplenium macrophyllum and Chrysosplenium flagelliferum (Saxifragaceae). Mitochondrial DNA B Resour. 5:2040–2041. 2020.PubMed/NCBI View Article : Google Scholar

10 

Choi HA, Ahn SO, Lim HD and Kim GJ: Growth suppression of a gingivitis and skin pathogen Cutibacterium (Propionibacterium) acnes by medicinal plant extracts. Antibiotics (Basel). 10(1092)2021.PubMed/NCBI View Article : Google Scholar

11 

Jakab J, Miškić B, Mikšić S, Juranić B, Ćosić V, Schwarz D and Včev A: Adipogenesis as a potential anti-obesity target: A review of pharmacological treatment and natural products. Diabetes Metab Syndr Obes. 14:67–83. 2021.PubMed/NCBI View Article : Google Scholar

12 

Madsen MS, Siersbaek R, Boergesen M, Nielsen R and Mandrup S: Peroxisome proliferator-activated receptor γ and C/EBPα synergistically activate key metabolic adipocyte genes by assisted loading. Mol Cell Biol. 34:939–954. 2014.PubMed/NCBI View Article : Google Scholar

13 

Barak Y, Nelson M, Ong E, Jones Y, Ruiz-Lozano P, Chien K, Koder A and Evans R: PPAR gamma is required for placental, cardiac, and adipose tissue development. Mol Cell. 4:585–595. 1999.PubMed/NCBI View Article : Google Scholar

14 

Rosen E, Sarraf P, Troy A, Bradwin G, Moore K, Milstone D, Spiegelman B and Mortensen R: PPAR gamma is required for the differentiation of adipose tissue in vivo and in vitro. Mol Cell. 4:611–617. 1999.PubMed/NCBI View Article : Google Scholar

15 

Duan SZ, Ivashchenko CY, Whitesall SE, D'Alecy LG, Duquaine DC, Brosius FC III, Gonzalez F, Vinson C, Pierre MA, Milstone DS and Mortensen RM: Hypotension, lipodystrophy, and insulin resistance in generalized PPARgamma-deficient mice rescued from embryonic lethality. J Clin Invest. 117:812–822. 2007.PubMed/NCBI View Article : Google Scholar

16 

Imai T, Takakuwa R, Marchand S, Dentz E, Bornert JM, Messaddeq N, Wendling O, Mark M, Desvergne B, Wahli W, et al: Peroxisome proliferator-activated receptor gamma is required in mature white and brown adipocytes for their survival in the mouse. Proc Natl Acad Sci USA. 101:4543–4547. 2004.PubMed/NCBI View Article : Google Scholar

17 

Linhart HG, Ishimura-Oka K, DeMayo F, Kibe T, Repka D, Poindexter B, Bick RJ and Darlington GJ: C/EBPalpha is required for differentiation of white, but not brown, adipose tissue. Proc Natl Acad Sci USA. 98:12532–12537. 2001.PubMed/NCBI View Article : Google Scholar

18 

Wang N, Finegold M, Bradley A, Ou C, Abdelsayed S, Wilde M, Taylor L, Wilson D and Darlington G: Impaired energy homeostasis in C/EBP alpha knockout mice. Science. 269:1108–1112. 1995.PubMed/NCBI View Article : Google Scholar

19 

Yang J, Croniger C, Lekstrom-Himes J, Zhang P, Fenyus M, Tenen D, Darlington G and Hanson R: Metabolic response of mice to a postnatal ablation of CCAAT/enhancer-binding protein alpha. J Biol Chem. 280:38689–38699. 2005.PubMed/NCBI View Article : Google Scholar

20 

Rosen E, Hsu CH, Wang X, Sakai S, Freeman M, Gonzalez F and Spiegelman B: C/EBPalpha induces adipogenesis through PPARgamma: A unified pathway. Genes Dev. 16:22–26. 2002.PubMed/NCBI View Article : Google Scholar

21 

Rosen ED and MacDougald OA: Adipocyte differentiation from the inside out. Nat Rev Mol Cell Biol. 7:885–896. 2006.PubMed/NCBI View Article : Google Scholar

22 

Chang E and Kim CY: Natural products and obesity: A focus on the regulation of mitotic clonal expansion during adipogenesis. Molecules. 24(1157)2019.PubMed/NCBI View Article : Google Scholar

23 

Yang XD, Ge XC, Jiang SY and Yang YY: Potential lipolytic regulators derived from natural products as effective approaches to treat obesity. Front Endocrinol (Lausanne). 13(1000739)2022.PubMed/NCBI View Article : Google Scholar

24 

Saponaro C, Gaggini M, Carli F and Gastaldelli A: The subtle balance between lipolysis and lipogenesis: A critical point in metabolic homeostasis. Nutrients. 7:9453–9474. 2015.PubMed/NCBI View Article : Google Scholar

25 

Song Z, Xiaoli AM and Yang F: Regulation and metabolic significance of de novo lipogenesis in adipose tissues. Nutrients. 10(1383)2018.PubMed/NCBI View Article : Google Scholar

26 

Brejchova K, Radner FPW, Balas L, Paluchova V, Cajka T, Chodounska H, Kudova E, Schratter M, Schreiber R, Durand T, et al: Distinct roles of adipose triglyceride lipase and hormone-sensitive lipase in the catabolism of triacylglycerol estolides. Proc Natl Acad Sci USA. 118(e2020999118)2021.PubMed/NCBI View Article : Google Scholar

27 

Mottillo EP and Granneman JG: Intracellular fatty acids suppress β-adrenergic induction of PKA-targeted gene expression in white adipocytes. Am J Physiol Endocrinol Metab. 301:E122–E131. 2011.PubMed/NCBI View Article : Google Scholar

28 

Chakrabarti P, English T, Karki S, Qiang L, Tao R, Kim J, Luo Z, Farmer SR and Kandror KV: SIRT1 controls lipolysis in adipocytes via FOXO1-mediated expression of ATGL. J Lipid Res. 52:1693–1701. 2011.PubMed/NCBI View Article : Google Scholar

29 

Barbato DL, Aquilano K, Baldelli S, Cannata SM, Bernardini S, Rotilio G and Ciriolo MR: Proline oxidase-adipose triglyceride lipase pathway restrains adipose cell death and tissue inflammation. Cell Death Differ. 21:113–123. 2014.PubMed/NCBI View Article : Google Scholar

30 

Granneman JG, Moore HP, Krishnamoorthy R and Rathod M: Perilipin controls lipolysis by regulating the interactions of AB-hydrolase containing 5 (Abhd5) and adipose triglyceride lipase (Atgl). J Biol Chem. 284:34538–34544. 2009.PubMed/NCBI View Article : Google Scholar

31 

Contreras GA, Strieder-Barboza C and Raphael W: Adipose tissue lipolysis and remodeling during the transition period of dairy cows. J Anim Sci Biotechnol. 8(41)2017.PubMed/NCBI View Article : Google Scholar

32 

Straub BK, Stoeffel P, Heid H, Zimbelmann R and Schirmacher P: Differential pattern of lipid droplet-associated proteins and de novo perilipin expression in hepatocyte teratogenesis. Hepatology. 47:1936–1946. 2008.PubMed/NCBI View Article : Google Scholar

33 

Temprano A, Sembongi H, Han GS, Sebastián D, Capellades J, Moreno C, Guardiola J, Wabitsch M, Richart C, Yanes O, et al: Redundant roles of the phosphatidate phosphatase family in triacylglycerol synthesis in human adipocytes. Diabetologia. 59:1985–1994. 2016.PubMed/NCBI View Article : Google Scholar

34 

Shin H, Shi H, Xue B and Yu L: What activates thermogenesis when lipid droplet lipolysis is absent in brown adipocytes? Adipocyte. 7:143–147. 2018.PubMed/NCBI View Article : Google Scholar

35 

Nguyen VTT, Vu VV and Pham PV: Brown adipocyte and browning thermogenesis: Metabolic crosstalk beyond mitochondrial limits and physiological impacts. Adipocytes. 12(2237164)2023.PubMed/NCBI View Article : Google Scholar

36 

Van der Vaart JI, Boon MR and Houtkooper RH: The role of AMPK signaling in brown adipose tissue activation. Cells. 10(1122)2021.PubMed/NCBI View Article : Google Scholar

37 

Gulyaeva O, Dempersmier J and Sul HS: Genetic and epigenetic control of adipose development. Biochim Biophys Acta Mol Cell Biol Lipids. 1864:3–12. 2019.PubMed/NCBI View Article : Google Scholar

38 

Hirayama D, Iida T and Nakase H: The phagocytic function of macrophage-enforcing innate immunity and tissue homeostasis. Int J Mol Sci. 19(92)2018.PubMed/NCBI View Article : Google Scholar

39 

Navegantes KC, de Souza Gomes R, Pereira PAT, Czaikoski PG, Azevedo CHM and Monteiro MC: Immune modulation of some autoimmune diseases: The critical role of macrophages and neutrophils in the innate and adaptive immunity. J Transl Med. 15(36)2017.PubMed/NCBI View Article : Google Scholar

40 

Lagos LS, Luu TV, De Haan B, Faas M and De Vos P: TLR2 and TLR4 activity in monocytes and macrophages after exposure to amoxicillin, ciprofloxacin, doxycycline and erythromycin. J Antimicrob Chemother. 77:2972–2983. 2022.PubMed/NCBI View Article : Google Scholar

41 

Wu J, Mo J, Xiang W, Shi X, Guo L, Li Y, Bao Y and Zheng L: Immunoregulatory effects of Tetrastigma hemsleyanum polysaccharide via TLR4-mediated NF-κB and MAPK signaling pathways in Raw264.7 macrophages. Biomed Pharmacother. 161(114471)2023.PubMed/NCBI View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Choi J, Park G, Choi H, Lee J, Kwon H, Choi M and Jeong J: Anti‑obesity and immunostimulatory activity of <em>Chrysosplenium flagelliferum</em> in mouse preadipocytes 3T3‑L1 cells and mouse macrophage RAW264.7 cells&nbsp;. Exp Ther Med 28: 315, 2024.
APA
Choi, J., Park, G., Choi, H., Lee, J., Kwon, H., Choi, M., & Jeong, J. (2024). Anti‑obesity and immunostimulatory activity of <em>Chrysosplenium flagelliferum</em> in mouse preadipocytes 3T3‑L1 cells and mouse macrophage RAW264.7 cells&nbsp;. Experimental and Therapeutic Medicine, 28, 315. https://doi.org/10.3892/etm.2024.12604
MLA
Choi, J., Park, G., Choi, H., Lee, J., Kwon, H., Choi, M., Jeong, J."Anti‑obesity and immunostimulatory activity of <em>Chrysosplenium flagelliferum</em> in mouse preadipocytes 3T3‑L1 cells and mouse macrophage RAW264.7 cells&nbsp;". Experimental and Therapeutic Medicine 28.2 (2024): 315.
Chicago
Choi, J., Park, G., Choi, H., Lee, J., Kwon, H., Choi, M., Jeong, J."Anti‑obesity and immunostimulatory activity of <em>Chrysosplenium flagelliferum</em> in mouse preadipocytes 3T3‑L1 cells and mouse macrophage RAW264.7 cells&nbsp;". Experimental and Therapeutic Medicine 28, no. 2 (2024): 315. https://doi.org/10.3892/etm.2024.12604
Copy and paste a formatted citation
x
Spandidos Publications style
Choi J, Park G, Choi H, Lee J, Kwon H, Choi M and Jeong J: Anti‑obesity and immunostimulatory activity of <em>Chrysosplenium flagelliferum</em> in mouse preadipocytes 3T3‑L1 cells and mouse macrophage RAW264.7 cells&nbsp;. Exp Ther Med 28: 315, 2024.
APA
Choi, J., Park, G., Choi, H., Lee, J., Kwon, H., Choi, M., & Jeong, J. (2024). Anti‑obesity and immunostimulatory activity of <em>Chrysosplenium flagelliferum</em> in mouse preadipocytes 3T3‑L1 cells and mouse macrophage RAW264.7 cells&nbsp;. Experimental and Therapeutic Medicine, 28, 315. https://doi.org/10.3892/etm.2024.12604
MLA
Choi, J., Park, G., Choi, H., Lee, J., Kwon, H., Choi, M., Jeong, J."Anti‑obesity and immunostimulatory activity of <em>Chrysosplenium flagelliferum</em> in mouse preadipocytes 3T3‑L1 cells and mouse macrophage RAW264.7 cells&nbsp;". Experimental and Therapeutic Medicine 28.2 (2024): 315.
Chicago
Choi, J., Park, G., Choi, H., Lee, J., Kwon, H., Choi, M., Jeong, J."Anti‑obesity and immunostimulatory activity of <em>Chrysosplenium flagelliferum</em> in mouse preadipocytes 3T3‑L1 cells and mouse macrophage RAW264.7 cells&nbsp;". Experimental and Therapeutic Medicine 28, no. 2 (2024): 315. https://doi.org/10.3892/etm.2024.12604
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