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Article Open Access

In situ mass spectrometry imaging reveals metabolite alterations in gray and white matter after spinal cord injury

  • Authors:
    • Chenghao Lyu
    • Yujing Tang
    • Fei-Fei Shang
  • View Affiliations / Copyright

    Affiliations: International Medical College and College of Basic Medicine, Chongqing Medical University, Chongqing 400016, P.R. China
    Copyright: © Lyu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 117
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    Published online on: April 11, 2025
       https://doi.org/10.3892/etm.2025.12867
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Abstract

It is known that spinal cord injury (SCI) causes metabolic disorders, such as disrupted lipid and amino acid metabolism. However, the in situ changes of metabolites in the spinal cord remain elusive. In the present study, adult Sprague‑Dawley rats underwent spinal cord transection surgery. Ambient air flow‑assisted desorption electrospray ionization mass spectrometry imaging was performed to identify the localization of metabolites in the white and gray matter and the ventral and dorsal horns within the spinal cord sections. The results revealed that 42 metabolites were specifically increased in the gray matter, and 90 metabolites were increased in the white matter compared with that in the sham group. In the ventral and dorsal horns, 86 and 103 metabolites, respectively, exhibited specific increases after injury. By contrast, numerous metabolites, especially lipids, were significantly decreased after SCI. Phosphatidylserine and phosphatidylethanolamine were mainly decreased in the gray matter, while cholesterol and ceramide were mainly decreased in the white matter. Specifically, phosphatidylethanolamine was detected at low levels in the dorsal horn following injury. However, the phosphatidylserine level decreased in the ventral horn. The functional enrichment of these metabolites via Kyoto Encyclopedia of Genes and Genomes analysis further confirmed the profile differences in the white and gray matter, as well as in the ventral and dorsal horns after SCI. These results provide valuable information on the metabolite profiles across various anatomical regions of the spinal cord following SCI, which may support the development of precise treatment strategies for patients.
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Copy and paste a formatted citation
Spandidos Publications style
Lyu C, Tang Y and Shang F: <em>In situ</em> mass spectrometry imaging reveals metabolite alterations in gray and white matter after spinal cord injury. Exp Ther Med 29: 117, 2025.
APA
Lyu, C., Tang, Y., & Shang, F. (2025). <em>In situ</em> mass spectrometry imaging reveals metabolite alterations in gray and white matter after spinal cord injury. Experimental and Therapeutic Medicine, 29, 117. https://doi.org/10.3892/etm.2025.12867
MLA
Lyu, C., Tang, Y., Shang, F."<em>In situ</em> mass spectrometry imaging reveals metabolite alterations in gray and white matter after spinal cord injury". Experimental and Therapeutic Medicine 29.6 (2025): 117.
Chicago
Lyu, C., Tang, Y., Shang, F."<em>In situ</em> mass spectrometry imaging reveals metabolite alterations in gray and white matter after spinal cord injury". Experimental and Therapeutic Medicine 29, no. 6 (2025): 117. https://doi.org/10.3892/etm.2025.12867
Copy and paste a formatted citation
x
Spandidos Publications style
Lyu C, Tang Y and Shang F: <em>In situ</em> mass spectrometry imaging reveals metabolite alterations in gray and white matter after spinal cord injury. Exp Ther Med 29: 117, 2025.
APA
Lyu, C., Tang, Y., & Shang, F. (2025). <em>In situ</em> mass spectrometry imaging reveals metabolite alterations in gray and white matter after spinal cord injury. Experimental and Therapeutic Medicine, 29, 117. https://doi.org/10.3892/etm.2025.12867
MLA
Lyu, C., Tang, Y., Shang, F."<em>In situ</em> mass spectrometry imaging reveals metabolite alterations in gray and white matter after spinal cord injury". Experimental and Therapeutic Medicine 29.6 (2025): 117.
Chicago
Lyu, C., Tang, Y., Shang, F."<em>In situ</em> mass spectrometry imaging reveals metabolite alterations in gray and white matter after spinal cord injury". Experimental and Therapeutic Medicine 29, no. 6 (2025): 117. https://doi.org/10.3892/etm.2025.12867
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