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![(A) Western blot analysis was
conducted to examine the fusion proteins of CRPNb, CRPNb-AP,
CRPNb-RHCC-AP, CRPNb-VTB-AP and CRPNb-C4bp-AP using purified
CRPNb-VTB-AP as a molecular weight standard for reference. (B)
Relative expression levels of CRPNb-AP, CRPNb-RHCC-AP, CRPNb-VTB-AP
and CRPNb-C4bp-AP. Proteins were quantified using purified
CRPNb-VTB-AP [lane P in (A)] as a control. The concentration of the
control protein CRPNb-VTB-AP was 240 µg/ml, as determined by a BCA
assay. Image Lab software was used for the intensity analysis.
CRPNb, C-reactive protein nanobody; AP, alkaline phosphatase; RHCC,
right-handed coiled coil; VTB, verotoxin; C4bp, C4-binding protein;
S, soluble; IS, insoluble; P, positive control.](/article_images/etm/32/4/etm-32-04-13279-g01.jpg)
