Fibroblast growth factor 2 inhibits the expression of stromal cell-derived factor 1α in periodontal ligament cells derived from human permanent teeth in vitro

Asakawa,Takeyoshi; Chosa,Naoyuki; Yoshimura,Yoshitaka; Asakawa,Asami; Tanaka,Mitsuro; Ishisaki,Akira; Mitome,Masato; Hasegawa,Tomokazu

doi:10.3892/ijmm.2011.869

April 2012 Volume 29 Issue 4

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April 2012 Volume 29 Issue 4

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Article

Fibroblast growth factor 2 inhibits the expression of stromal cell-derived factor 1α in periodontal ligament cells derived from human permanent teeth in vitro

Authors:
- Takeyoshi Asakawa
- Naoyuki Chosa
- Yoshitaka Yoshimura
- Asami Asakawa
- Mitsuro Tanaka
- Akira Ishisaki
- Masato Mitome
- Tomokazu Hasegawa
View Affiliations / Copyright

Affiliations: Department of Pediatric Dentistry, School of Dentistry, Iwate Medical University, Morioka 020-8505, Japan, Department of Pediatric Dentistry, Tokushima University Hospital, 3-18-15 Kuramoto, Tokushima 770-8504, Japan
Pages: 569-573
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Published online on: December 23, 2011

https://doi.org/10.3892/ijmm.2011.869
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Abstract

Although cells derived from periodontal ligament (PDL) tissue are reported to have stem cell-like activity and are speculated to play a crucial role for tissue healing and regeneration after injury or orthodontic treatment, mechanisms regulating their recruitment and activation remain unknown. Recently, stromal cell-derived factor 1α (SDF-1α) has been reported to be important for stem cell homing and recruitment to injured sites. The aim of this study was to evaluate whether fibroblast growth factor 2 (FGF-2) affects the expression of SDF-1α in PDL cells derived from human permanent teeth in vitro. Using real-time PCR, the expression of SDF-1α mRNA in PDL cells was inhibited by treatment with 10 ng/ml FGF-2. When PDL cells were treated with SU5402 (an inhibitor of FGF receptor 1) in combination with FGF-2, the FGF-2-reduced expression of SDF-1α was inhibited. In the presence of the JNK inhibitor SP600125, SDF-1α mRNA in PDL cells was not suppressed by the FGF-2 treatment. Western blot analysis also showed that SDF-1α production was suppressed by treatment with FGF-2, but it recovered with treatment by FGF-2 + SU5402. These findings suggest that SDF-1α from PDL cells plays an important role in the regeneration and homeostasis of periodontal tissues via the recruitment of stem cells.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Fibroblast growth factor 2 inhibits the expression of stromal cell-derived factor 1α in periodontal ligament cells derived from human permanent teeth in vitro

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