Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
International Journal of Molecular Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1107-3756 Online ISSN: 1791-244X
Journal Cover
September 2012 Volume 30 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
September 2012 Volume 30 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Establishment of immortalized Schwann cells derived from rat embryo dorsal root ganglia

  • Authors:
    • Huajun Jiang
    • Wei Qu
    • Feng Han
    • Dazhuang Liu
    • Weiguo Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedics, First Affiliated Hospital of Dalian Medical University, Dalian 116011, P.R. China
    Copyright: © Jiang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 480-486
    |
    Published online on: June 6, 2012
       https://doi.org/10.3892/ijmm.2012.1016
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Schwann cells (SCs) play an important role in the development, function and regeneration of peripheral nerves. They can enhance both peripheral and central nerve regeneration by providing a supportive environment for neurite outgrowth through the release of neurotrophic factors. However, use of primary SCs for in vitro models is limited because these cells are difficult to prepare and maintain in high yield and purity under common cell culture conditions. Human telomerase reverse transcriptase (hTERT) expression induces immortalization of various cell types without substantial alterations of their phenotypes. Therefore, in this study we transfected SCs with hTERT to establish a reliable cell source and observed the effect of hTERT on SCs. In order to accomplish this, SCs were isolated from rat embryo dorsal root ganglions, transfected with hTERT at early passage (passage 3). SCs passage 4, 8, 12 and 30 after transfection (hTERT-SCs) were used for immunocytochemistry, RT-PCR and western blotting. Results showed that all the early (passage 4) and late (passage 30) passage hTERT-SCs expressed hTERT mRNA and gained full telomerase activity. The transfection did not alter the mRNA expression of senescence-associated genes, such as p53 and p16. The expression of BDNF (brain-derived neurotrophic factor) was significantly decreased as cell passage increased, compared to the untransfected control. On the other hand, the expression of NGF (nerve growth factor ) was elevated at early passages (passages 4 and 8) and decreased at late passages (12 and 30). These data indicate that the use of specific immortalization techniques can establish SC lines that retain characteristics of typical primary SCs, and different mechanisms responsible for regulating NGF and BDNF expression. This is the first report regarding the immortalization of SCs derived from rat embryo dorsal root ganglions. These cells are useful in studies investigating the cellular mechanisms and regenerative processes of SCs.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1. 

SE MackinnonAL DellonA study of nerve regeneration across synthetic (Maxon) and biologic (collagen) nerve conduits for nerve gaps up to 5 cm in the primateJ Reconstr Microsurg6117121199010.1055/s-2007-10068102352218

2. 

S TechangamsuwanR KreutzerM KreutzerI ImbschweilerK RohnK WewetzerW BaumgärtnerTransfection of adult canine Schwann cells and olfactory ensheathing cells at early and late passage with human TERT differentially affects growth factor responsiveness and in vitro growthJ Neurosci Methods176112120200910.1016/j.jneumeth.2008.08.030

3. 

HC LehmannW ChenR MiS WangY LiuM RaoA HökeHuman Schwann cells retain essential phenotype characteristics after immortalizationStem Cells Dev21423431201210.1089/scd.2010.0513

4. 

Y WeiJ ZhouZ ZhengA WangQ AoY GongX ZhangAn improved method for isolating Schwann cells from postnatal rat sciatic nervesCell Tissue Res337361369200910.1007/s00441-009-0836-419639342

5. 

A NiapourF KaramaliK KarbalaieA KianiM MardaniMH Nasr-EsfahaniH BaharvandNovel method to obtain highly enriched cultures of adult rat Schwann cellsBiotechnol Lett32781786201010.1007/s10529-010-0230-z20213527

6. 

T KomiyamaY NakaoY ToyamaH AsouCA VacantiMP VacantiA novel technique to isolate adult Schwann cells for an artificial nerve conduitJ Neurosci Methods122195200200310.1016/S0165-0270(02)00320-512573478

7. 

WE WrightJW ShayMA PiatyszekModifications of a telomeric repeat amplification protocol (TRAP) result in increased reliability, linearity and sensitivityNucleic Acids Res2337943795199510.1093/nar/23.18.3794

8. 

JW FawcettRJ KeynesPeripheral nerve regenerationAnnu Rev Neurosci134360199010.1146/annurev.neuro.13.1.43

9. 

MR FeneleyJW FawcettRJ KeynesThe role of Schwann cells in the regeneration of peripheral nerve axons through muscle basal lamina graftsExp Neurol114275285199110.1016/0014-4886(91)90153-41748202

10. 

K HaastertJ GrosskreutzM JaeckelC LadererJ BuflerC GrotheP ClausRat embryonic motoneurons in long-term co-culture with Schwann cells - a system to investigate motoneuron diseases on a cellular level in vitroJ Neurosci Methods142275284200510.1016/j.jneumeth.2004.09.003

11. 

K RothblumRC StahlDJ CareyConstitutive release of alpha4 type V collagen N-terminal domain by Schwann cells and binding to cell surface and extracellular matrix heparan sulfate proteoglycansJ Biol Chem2795128251288200410.1074/jbc.M408837200

12. 

Y ShibuyaA MizoguchiM TakeichiK ShimadaC IdeLocalization of N-cadherin in the normal and regenerating nerve fibers of the chicken peripheral nervous systemNeuroscience67253261199510.1016/0306-4522(95)00015-B7477906

13. 

A Baron-Van EvercoorenV Avellana-AdalidF LachapelleR LiblauSchwann cell transplantation and myelin repair of the CNSMult Scler315716119979291173

14. 

YY ChenD McDonaldC ChengB MagnowskiJ DurandDW ZochodneAxon and Schwann cell partnership during nerve regrowthJ Neuropathol Exp Neurol64613622200510.1097/01.jnen.0000171650.94341.4616042313

15. 

A MosahebiB WoodwardM WibergR MartinG TerenghiRetroviral labeling of Schwann cells: in vitro characterization and in vivo transplantation to improve peripheral nerve regenerationGlia34817200110.1002/glia.103511284015

16. 

JL RutkowskiCJ KirkMA LernerGI TennekoonPurification and expansion of human Schwann cells in vitroNat Med18083199510.1038/nm0195-807584959

17. 

TK MorrisseyN KleitmanRP BungeHuman Schwann cells in vitro. II. Myelination of sensory axons following extensive purification and heregulin-induced expansionJ Neurobiol28190201199510.1002/neu.480280206

18. 

TK MorrisseyRP BungeN KleitmanHuman Schwann cells in vitro. I Failure to differentiate and support neuronal health under co-culture conditions that promote full function of rodent cellsJ Neurobiol28171189199510.1002/neu.480280205

19. 

XR JiangG JimenezE ChangM FrolkisB KuslerM SageM BeecheAG BodnarGM WahlTD TlstyCP ChiuTelomerase expression in human somatic cells does not induce changes associated with a transformed phenotypeNat Genet21111114199910.1038/50569916802

20. 

CP MoralesSE HoltM OuelletteKJ KaurY YanKS WilsonMA WhiteWE WrightJW ShayAbsence of cancer-associated changes in human fibroblasts immortalized with telomeraseNat Genet21115118199910.1038/50639916803

21. 

MM OuelletteLD McDanielWE WrightJW ShayRA SchultzThe establishment of telomerase-immortalized cell lines representing human chromosome instability syndromesHum Mol Genet9403411200010.1093/hmg/9.3.40310655550

22. 

DJ ArgyleL NasirTelomerase: a potential diagnostic and therapeutic tool in canine oncologyVet Pathol4017200310.1354/vp.40-1-112627707

23. 

L NasirTelomeres and telomerase: Biological and clinical importance in dogsVet J175155163200810.1016/j.tvjl.2007.01.02417398127

24. 

V GorbunovaA SeluanovOM Pereira-SmithEvidence that high telomerase activity may induce a senescent-like growth arrest in human fibroblastsJ Biol Chem27876927698200310.1074/jbc.M21294420012496279

25. 

LL SmithHA CollerJM RobertsTelomerase modulates expression of growth-controlling genes and enhances cell proliferationNat Cell Biol5474479200310.1038/ncb98512717449

26. 

JI YoungJM SedivyJR SmithTelomerase expression in normal human fibroblasts stabilizes DNA 5-methylcytosine transferase IJ Biol Chem2781990419908200310.1074/jbc.M30168520012665523

27. 

X JinJS LeeS KwakSY LeeJE JungTK KimC XuZ HongZ LiSM KimEstablishment and characterization of three immortal bovine muscular epithelial cell linesMol Cells212933200616511344

28. 

M OudegaXM XuSchwann cell transplantation for repair of the adult spinal cordJ Neurotrauma23453467200610.1089/neu.2006.23.45316629629

29. 

R HeumannS KorschingC BandtlowH ThoenenChanges of nerve growth factor synthesis in nonneuronal cells in response to sciatic nerve transectionJ Cell Biol10416231631198710.1083/jcb.104.6.16233034917

30. 

A AchesonPA BarkerRF AldersonFD MillerRA MurphyDetection of brain-derived neurotrophic factor-like activity in fibroblasts and Schwann cells: inhibition by antibodies to NGFNeuron7265275199110.1016/0896-6273(91)90265-21873030

31. 

N OffenhauserR Böhm-MatthaeiP TsoulfasL ParadaM MeyerDevelopmental regulation of full-length trkC in the rat sciatic nerveEur J Neurosci7917925199510.1111/j.1460-9568.1995.tb01079.x7613627

32. 

H ZhuF LiWJ YuWJ WangL LiLD WanY LeWL DingEffect of hypoxia/reoxygenation on cell viability and expression and secretion of neurotrophic factors (NTFs) in primary cultured schwann cellsAnat Rec (Hoboken)293865870201010.1002/ar.2110520186961

33. 

N OkaT KawasakiK MizutaniH SugiyamaI AkiguchiHypoxia-inducible factor 1alpha may be a marker for vasculitic neuropathyNeuropathology27509515200710.1111/j.1440-1789.2007.00817.x18021370

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Jiang H, Qu W, Han F, Liu D and Zhang W: Establishment of immortalized Schwann cells derived from rat embryo dorsal root ganglia. Int J Mol Med 30: 480-486, 2012.
APA
Jiang, H., Qu, W., Han, F., Liu, D., & Zhang, W. (2012). Establishment of immortalized Schwann cells derived from rat embryo dorsal root ganglia. International Journal of Molecular Medicine, 30, 480-486. https://doi.org/10.3892/ijmm.2012.1016
MLA
Jiang, H., Qu, W., Han, F., Liu, D., Zhang, W."Establishment of immortalized Schwann cells derived from rat embryo dorsal root ganglia". International Journal of Molecular Medicine 30.3 (2012): 480-486.
Chicago
Jiang, H., Qu, W., Han, F., Liu, D., Zhang, W."Establishment of immortalized Schwann cells derived from rat embryo dorsal root ganglia". International Journal of Molecular Medicine 30, no. 3 (2012): 480-486. https://doi.org/10.3892/ijmm.2012.1016
Copy and paste a formatted citation
x
Spandidos Publications style
Jiang H, Qu W, Han F, Liu D and Zhang W: Establishment of immortalized Schwann cells derived from rat embryo dorsal root ganglia. Int J Mol Med 30: 480-486, 2012.
APA
Jiang, H., Qu, W., Han, F., Liu, D., & Zhang, W. (2012). Establishment of immortalized Schwann cells derived from rat embryo dorsal root ganglia. International Journal of Molecular Medicine, 30, 480-486. https://doi.org/10.3892/ijmm.2012.1016
MLA
Jiang, H., Qu, W., Han, F., Liu, D., Zhang, W."Establishment of immortalized Schwann cells derived from rat embryo dorsal root ganglia". International Journal of Molecular Medicine 30.3 (2012): 480-486.
Chicago
Jiang, H., Qu, W., Han, F., Liu, D., Zhang, W."Establishment of immortalized Schwann cells derived from rat embryo dorsal root ganglia". International Journal of Molecular Medicine 30, no. 3 (2012): 480-486. https://doi.org/10.3892/ijmm.2012.1016
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team