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September 2012 Volume 30 Issue 3

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Article

siRNA-based targeting of fractalkine overexpression suppresses inflammation development in a severe acute pancreatitis rat model

  • Authors:
    • Liya Huang
    • Junwen Ma
    • Yuming Tang
    • Ping Chen
    • Shuxian Zhang
    • Yongping Zhang
    • Yao Zong Yuan
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, P.R. China
  • Pages: 514-520
    |
    Published online on: June 29, 2012
       https://doi.org/10.3892/ijmm.2012.1050
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Abstract

Fractalkine (FKN), a chemokine that acts as both an adhesion molecule and a chemoattractant, is expressed in many inflammatory diseases. Chemokines play a crucial role in severe acute pancreatitis (SAP). This study used adenovirus-mediated siRNA to target FKN overexpression and assessed its ability to suppress inflammation development in a SAP rat model. Adenovirus-mediated FKN siRNA was transfected into cerulein-stimulated AR42J cells. The growth of cerulein-stimulated AR42J cells was determined by colony formation and MTT assays. The inhibitory effect of the FKN siRNA was studied in a SAP rat model in vivo and detected by ELISA, RT-PCR, western blot analysis and immunohistochemistry. FKN, IL-8 and TNF-α were found to be overexpressed in cerulein-stimulated AR42J cells by ELISA and western blot analysis (P<0.05). The animal experiments confirmed that FKN siRNA could inhibit inflammation development in SAP. The values of serum FKN, TNF-α and IL-8 levels were decreased after FKN siRNA treatment (P<0.05). Furthermore, western blotting and RT-PCR analysis showed that FKN protein and mRNA levels were decreased after injection with FKN siRNA (P<0.05). Immunohistochemistry also showed that inflammation was decreased after injection with FKN-siRNA in the SAP rat model. Treatment with siRNA can inhibit FKN overexpression and also suppresses inflammation development in a SAP rat model. More importantly, this study indicated that FKN, which is overexpressed in the SAP rat model, may serve as a novel and effective therapeutic target for SAP.
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Copy and paste a formatted citation
Spandidos Publications style
Huang L, Ma J, Tang Y, Chen P, Zhang S, Zhang Y and Yuan YZ: siRNA-based targeting of fractalkine overexpression suppresses inflammation development in a severe acute pancreatitis rat model. Int J Mol Med 30: 514-520, 2012.
APA
Huang, L., Ma, J., Tang, Y., Chen, P., Zhang, S., Zhang, Y., & Yuan, Y.Z. (2012). siRNA-based targeting of fractalkine overexpression suppresses inflammation development in a severe acute pancreatitis rat model. International Journal of Molecular Medicine, 30, 514-520. https://doi.org/10.3892/ijmm.2012.1050
MLA
Huang, L., Ma, J., Tang, Y., Chen, P., Zhang, S., Zhang, Y., Yuan, Y. Z."siRNA-based targeting of fractalkine overexpression suppresses inflammation development in a severe acute pancreatitis rat model". International Journal of Molecular Medicine 30.3 (2012): 514-520.
Chicago
Huang, L., Ma, J., Tang, Y., Chen, P., Zhang, S., Zhang, Y., Yuan, Y. Z."siRNA-based targeting of fractalkine overexpression suppresses inflammation development in a severe acute pancreatitis rat model". International Journal of Molecular Medicine 30, no. 3 (2012): 514-520. https://doi.org/10.3892/ijmm.2012.1050
Copy and paste a formatted citation
x
Spandidos Publications style
Huang L, Ma J, Tang Y, Chen P, Zhang S, Zhang Y and Yuan YZ: siRNA-based targeting of fractalkine overexpression suppresses inflammation development in a severe acute pancreatitis rat model. Int J Mol Med 30: 514-520, 2012.
APA
Huang, L., Ma, J., Tang, Y., Chen, P., Zhang, S., Zhang, Y., & Yuan, Y.Z. (2012). siRNA-based targeting of fractalkine overexpression suppresses inflammation development in a severe acute pancreatitis rat model. International Journal of Molecular Medicine, 30, 514-520. https://doi.org/10.3892/ijmm.2012.1050
MLA
Huang, L., Ma, J., Tang, Y., Chen, P., Zhang, S., Zhang, Y., Yuan, Y. Z."siRNA-based targeting of fractalkine overexpression suppresses inflammation development in a severe acute pancreatitis rat model". International Journal of Molecular Medicine 30.3 (2012): 514-520.
Chicago
Huang, L., Ma, J., Tang, Y., Chen, P., Zhang, S., Zhang, Y., Yuan, Y. Z."siRNA-based targeting of fractalkine overexpression suppresses inflammation development in a severe acute pancreatitis rat model". International Journal of Molecular Medicine 30, no. 3 (2012): 514-520. https://doi.org/10.3892/ijmm.2012.1050
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