Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
International Journal of Molecular Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1107-3756 Online ISSN: 1791-244X
Journal Cover
October 2012 Volume 30 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
October 2012 Volume 30 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Interleukin-18-induced inflammatory responses in synoviocytes and chondrocytes from osteoarthritic patients

  • Authors:
    • Zhaozong Fu
    • Peng Liu
    • Dehong Yang
    • Fenglong Wang
    • Liang Yuan
    • Zhen Lin
    • Jianming Jiang
  • View Affiliations / Copyright

    Affiliations: Department of Spine Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, P.R. China
  • Pages: 805-810
    |
    Published online on: July 20, 2012
       https://doi.org/10.3892/ijmm.2012.1073
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The major pathological changes of osteoarthritis (OA) include cartilage degeneration and synovial inflammation. Previous studies confirmed that interleukin-1 (IL-1) stimulates the secretion of multiple inflammatory factors in synoviocytes and chondrocytes. IL-18 is a member of the IL-1 superfamily. In this study, the pro-inflammatory effects of IL-18 on synoviocytes and chondrocytes in patients with OA were investigated. Knee synovial membrane and cartilage samples were obtained from OA patients, then primary cells were cultured. Synoviocytes and primary chondrocytes at different generations (primary, secondary and tertiary), were stimulated with IL-18, then inflammatory marker levels, including tumor necrosis factor-α (TNF-α), prostaglandin E2 (PGE2) and cyclooxygenase-2 (COX-2), were measured using reverse transcription polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay. IL-1 receptor antagonist (IL-1Ra) was applied to interfere with the IL-18 stimulation of chondrocytes, and then the COX-2 expression in chondrocytes and the PGE2 levels in the medium were measured. The expression of IL-18 receptor α (IL-18Rα) and IL-18 receptor β (IL-18Rβ) in synoviocytes and chondrocytes was assessed, using RT-PCR. Our results showed that IL-18 stimulated the COX-2 and TNF-α expressions in primary synoviocytes, while increasing PGE2 and TNF-α levels in the supernatant (P<0.05) of the culture medium in primary synoviocytes. IL-18 also induced high PGE2 level production in second-generation synoviocytes (P<0.05). Moreover, IL-18 upregulated COX-2 and TNF-α mRNA in chondrocytes, while promoting PGE2 and TNF-α (P<0.05) secretions in a dose-dependent manner. The induced effects were not attenuated by the addition of IL-1Ra (P<0.05). IL-18Rα was expressed in the chondrocytes and synoviocytes of 4/8 patients, while IL-18Rβ was expressed in the chondrocytes of 4/8 patients and in the synoviocytes of 2/8 patients. We conclude that IL-18 induces inflammatory responses in synoviocytes and chondrocytes and that this effect was correlated with, although not entirely dependent on, IL-1β.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1. 

MK HaynesEL HumeJB SmithPhenotypic characterization of inflammatory cells from osteoarthritic synovium and synovial fluidsClin Immunol105315325200210.1006/clim.2002.528312498813

2. 

MD SmithS TriantafillouA ParkerPP YoussefM ColemanSynovial membrane inflammation and cytokine production in patients with early osteoarthritisJ Rheumatol2436537119979034998

3. 

CA DinarelloThe IL-1 family and inflammatory diseasesClin Exp Rheumatol20S1S13200214989423

4. 

GL BjornssonL ThorsteinssonKO GudmundssonH Jonsson JrS GudmundssonB GudbjornssonInflammatory cytokines in relation to adrenal response following total hip replacementScand J Immunol6599105200710.1111/j.1365-3083.2006.01872.x17212773

5. 

SM DaiZZ ShanH XuK NishiokaCellular targets of interleukin-18 in rheumatoid arthritisAnn Rheum Dis6614111418200710.1136/ard.2006.06779317502360

6. 

M MohtaiMK GuptaB DonlonExpression of interleukin-6 in osteoarthritic chondrocytes and effects of fluid-induced shear on this expression in normal human chondrocytes in vitroJ Orthop Res146773199610.1002/jor.11001401128618168

7. 

H OkamuraH TsutsiT KomatsuCloning of a new cytokine that induces IFN-gamma production by T cellsNature3788891199510.1038/378088a07477296

8. 

T GhayurS BanerjeeM HuguninCaspase-1 processes IFN-gamma-inducing factor and regulates LPS-induced IFN-gamma productionNature386619623199710.1038/386619a09121587

9. 

K MatsuiH TsutsuiK NakanishiPathophysiological roles for IL-18 in inflammatory arthritisExpert Opin Ther Targets7701724200310.1517/14728222.7.6.70114640907

10. 

A PawlikM KurzawskiM DrozdzikV DziedziejkoK SafranowM HerczynskaInterleukin-18 gene (IL-18) promoter polymorphisms in patients with rheumatoid arthritisScand J Rheumatol38159165200910.1080/0300974080260074819229765

11. 

XT ShaoL FengLJ GuExpression of interleukin-18, IL-18BP, and IL-18R in serum, synovial fluid, and synovial tissue in patients with rheumatoid arthritisClin Exp Med9215221200910.1007/s10238-009-0036-219225717

12. 

B MollerN Kukoc-ZivojnovU KesslerExpression of interleukin-18 and its monokine-directed function in rheumatoid arthritisRheumatology (Oxford)40302309200110.1093/rheumatology/40.3.30211285378

13. 

M LotzFJ BlancoJ von KempisCytokine regulation of chondrocyte functionsJ Rheumatol Suppl431041081995

14. 

T OleeS HashimotoJ QuachM LotzIL-18 is produced by articular chondrocytes and induces proinflammatory and catabolic responsesJ Immunol1621096110019999916738

15. 

LA JoostenRL SmeetsMI KoendersInterleukin-18 promotes joint inflammation and induces interleukin-1-driven cartilage destructionAm J Pathol165959967200410.1016/S0002-9440(10)63357-315331419

16. 

T ZimmermannE KunischR PfeifferIsolation and characterization of rheumatoid arthritis synovial fibroblasts from primary culture - primary culture cells markedly differ from fourth-passage cellsArthritis Res37276200110.1186/ar142

17. 

S FrankeM SommerC RusterAdvanced glycation end products induce cell cycle arrest and proinflammatory changes in osteoarthritic fibroblast-like synovial cellsArthritis Res Ther11R136200910.1186/ar280719735566

18. 

KJ LivakTD SchmittgenAnalysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) methodMethods25402408200110.1006/meth.2001.126211846609

19. 

B MollerU KesslerS RehartExpression of interleukin-18 receptor in fibroblast-like synoviocytesArthritis Res4139144200210.1186/ar39011879550

20. 

WN UengCC ChuangCH ShihDouble-rib composite free transfer to reconstruct a single-spared lower extremity defectJ Trauma38210212199510.1097/00005373-199502000-000107869437

21. 

M GotoM SasanoH YamanakaSpontaneous production of an interleukin 1-like factor by cloned rheumatoid synovial cells in long-term cultureJ Clin Invest80786796198710.1172/JCI1131352442197

22. 

SW UengDC ChuangSL ChengCH ShihManagement of large infected tibial defects with radical debridement and staged double-rib composite free transferJ Trauma40345350199610.1097/00005373-199603000-000038601847

23. 

JA GracieRJ ForseyWL ChanA proinflammatory role for IL-18 in rheumatoid arthritisJ Clin Invest10413931401199910.1172/JCI731710562301

24. 

C Plater-ZyberkLA JoostenMM HelsenTherapeutic effect of neutralizing endogenous IL-18 activity in the collagen-induced model of arthritisJ Clin Invest10818251832200110.1172/JCI20011209711748266

25. 

MB GoldringM OteroDA PlumbRoles of inflammatory and anabolic cytokines in cartilage metabolism: signals and multiple effectors converge upon MMP-13 regulation in osteoarthritisEur Cell Mater21202220201121351054

26. 

X LiM EllmanP MuddasaniProstaglandin E2 and its cognate EP receptors control human adult articular cartilage homeostasis and are linked to the pathophysiology of osteoarthritisArthritis Rheum60513523200910.1002/art.2425819180509

27. 

BY ChanES FullerAK RussellIncreased chondrocyte sclerostin may protect against cartilage degradation in osteoarthritisOsteoarthritis Cartilage19874885201110.1016/j.joca.2011.04.01421619935

28. 

VA MaganevRA DavletshinGK DavletshinaGS IapparovDynamics of tumour necrosis factor-alpha and clinical signs of osteoarthrosis during the treatment with alflutop in combination with peloid applications under conditions of health resortVopr Kurortol Fizioter Lech Fiz Kult18202011(In Russian)

29. 

S OritaT KoshiT MitsukaAssociations between proinflammatory cytokines in the synovial fluid and radiographic grading and pain-related scores in 47 consecutive patients with osteoarthritis of the kneeBMC Musculoskeletal Disord12144201110.1186/1471-2474-12-144

30. 

M KawashimaP MiossecHeterogeneity of response of rheumatoid synovium cell subsets to interleukin-18 in relation to differential interleukin-18 receptor expressionArthritis Rheum48631637200310.1002/art.1082512632414

31. 

H LimHP KimMatrix metalloproteinase-13 expression in IL-1β-treated chondrocytes by activation of the p38 MAPK/c-Fos/AP-1 and JAK/STAT pathwaysArch Pharm Res341091172011

32. 

R SinghS AhmedCJ MalemudVM GoldbergTM HaqqiEpigallocatechin-3-gallate selectively inhibits interleukin-1beta-induced activation of mitogen activated protein kinase subgroup c-Jun N-terminal kinase in human osteoarthritis chondrocytesJ Orthop Res21102109200310.1016/S0736-0266(02)00089-X

33. 

B ChandrasekarS MummidiL MahimainathanInterleukin-18-induced human coronary artery smooth muscle cell migration is dependent on NF-kappaB- and AP-1-mediated matrix metalloproteinase-9 expression and is inhibited by atorvastatinJ Biol Chem2811509915109200610.1074/jbc.M600200200

34. 

JK LeeSH KimEC LewisT AzamLL ReznikovCA DinarelloDifferences in signaling pathways by IL-1beta and IL-18Proc Natl Acad Sci USA10188158820200410.1073/pnas.040280010115161979

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Fu Z, Liu P, Yang D, Wang F, Yuan L, Lin Z and Jiang J: Interleukin-18-induced inflammatory responses in synoviocytes and chondrocytes from osteoarthritic patients. Int J Mol Med 30: 805-810, 2012.
APA
Fu, Z., Liu, P., Yang, D., Wang, F., Yuan, L., Lin, Z., & Jiang, J. (2012). Interleukin-18-induced inflammatory responses in synoviocytes and chondrocytes from osteoarthritic patients. International Journal of Molecular Medicine, 30, 805-810. https://doi.org/10.3892/ijmm.2012.1073
MLA
Fu, Z., Liu, P., Yang, D., Wang, F., Yuan, L., Lin, Z., Jiang, J."Interleukin-18-induced inflammatory responses in synoviocytes and chondrocytes from osteoarthritic patients". International Journal of Molecular Medicine 30.4 (2012): 805-810.
Chicago
Fu, Z., Liu, P., Yang, D., Wang, F., Yuan, L., Lin, Z., Jiang, J."Interleukin-18-induced inflammatory responses in synoviocytes and chondrocytes from osteoarthritic patients". International Journal of Molecular Medicine 30, no. 4 (2012): 805-810. https://doi.org/10.3892/ijmm.2012.1073
Copy and paste a formatted citation
x
Spandidos Publications style
Fu Z, Liu P, Yang D, Wang F, Yuan L, Lin Z and Jiang J: Interleukin-18-induced inflammatory responses in synoviocytes and chondrocytes from osteoarthritic patients. Int J Mol Med 30: 805-810, 2012.
APA
Fu, Z., Liu, P., Yang, D., Wang, F., Yuan, L., Lin, Z., & Jiang, J. (2012). Interleukin-18-induced inflammatory responses in synoviocytes and chondrocytes from osteoarthritic patients. International Journal of Molecular Medicine, 30, 805-810. https://doi.org/10.3892/ijmm.2012.1073
MLA
Fu, Z., Liu, P., Yang, D., Wang, F., Yuan, L., Lin, Z., Jiang, J."Interleukin-18-induced inflammatory responses in synoviocytes and chondrocytes from osteoarthritic patients". International Journal of Molecular Medicine 30.4 (2012): 805-810.
Chicago
Fu, Z., Liu, P., Yang, D., Wang, F., Yuan, L., Lin, Z., Jiang, J."Interleukin-18-induced inflammatory responses in synoviocytes and chondrocytes from osteoarthritic patients". International Journal of Molecular Medicine 30, no. 4 (2012): 805-810. https://doi.org/10.3892/ijmm.2012.1073
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team