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International Journal of Molecular Medicine
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October 2012 Volume 30 Issue 4

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Article

Role of transcription factor Sp1 in the quercetin-mediated inhibitory effect on human malignant pleural mesothelioma

  • Authors:
    • Jung-Il Chae
    • Jin Hyoung Cho
    • Kyung-Ae Lee
    • Nag-Jin Choi
    • Kang Seok Seo
    • Sang-Bum Kim
    • Sang-Han Lee
    • Jung-Hyun Shim
  • View Affiliations / Copyright

    Affiliations: Department of Dental Pharmacology, School of Dentistry, Brain Korea 21 Project, Chonbuk National University, Jeonju 561-756, Republic of Korea, Department of Biochemistry, College of Medicine, Soonchunhyang University, Cheonan 330-090, Republic of Korea, Department of Animal Science, College of Agricultural and Life Science, Chonbuk National University, Jeonju 561-756, Republic of Korea, Department of Animal Science and Technology, Sunchon National University, Suncheon 540-742, Republic of Korea, Animal Genomics and Bioinformatics Division, National Institute of Animal Science, Rural Development Administration (RDA), Suwon 441-706, Republic of Korea
  • Pages: 835-841
    |
    Published online on: July 24, 2012
       https://doi.org/10.3892/ijmm.2012.1075
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Abstract

Quercetin (Qu) is found in plants, including red onions and in the skins of red apples, and induces the apoptosis of certain malignant cells. However, no report has been issued on the apoptotic effect of Qu on human malignant pleural mesothelioma. In the present study, it was found that MSTO‑211H mesothelioma cell viability was reduced and apoptotic cell death was increased by Qu (20-80 µM), which was found to have an IC50 of 58 µM. In addition, Qu increased the sub-G1 cell population, and was found to interact with specificity protein 1 (Sp1) and significantly suppressed its expression at the protein and mRNA levels. Furthermore, Qu modulated the levels of Sp1 regulatory genes, such as cyclin D1, myeloid cell leukemia (Mcl)-1 and survivin in MSTO-211H cells. Apoptotic signaling cascades were activated by the cleavage of Bid, caspase-3 and PARP, and by the downregulation of Bcl-xL and the upregulation of Bax in MSTO-211H cells. Our results strongly suggest that Sp1 be considered as a novel molecular target of Qu in human malignant pleural mesothelioma.
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Copy and paste a formatted citation
Spandidos Publications style
Chae J, Cho JH, Lee K, Choi N, Seo KS, Kim S, Lee S and Shim J: Role of transcription factor Sp1 in the quercetin-mediated inhibitory effect on human malignant pleural mesothelioma. Int J Mol Med 30: 835-841, 2012.
APA
Chae, J., Cho, J.H., Lee, K., Choi, N., Seo, K.S., Kim, S. ... Shim, J. (2012). Role of transcription factor Sp1 in the quercetin-mediated inhibitory effect on human malignant pleural mesothelioma. International Journal of Molecular Medicine, 30, 835-841. https://doi.org/10.3892/ijmm.2012.1075
MLA
Chae, J., Cho, J. H., Lee, K., Choi, N., Seo, K. S., Kim, S., Lee, S., Shim, J."Role of transcription factor Sp1 in the quercetin-mediated inhibitory effect on human malignant pleural mesothelioma". International Journal of Molecular Medicine 30.4 (2012): 835-841.
Chicago
Chae, J., Cho, J. H., Lee, K., Choi, N., Seo, K. S., Kim, S., Lee, S., Shim, J."Role of transcription factor Sp1 in the quercetin-mediated inhibitory effect on human malignant pleural mesothelioma". International Journal of Molecular Medicine 30, no. 4 (2012): 835-841. https://doi.org/10.3892/ijmm.2012.1075
Copy and paste a formatted citation
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Spandidos Publications style
Chae J, Cho JH, Lee K, Choi N, Seo KS, Kim S, Lee S and Shim J: Role of transcription factor Sp1 in the quercetin-mediated inhibitory effect on human malignant pleural mesothelioma. Int J Mol Med 30: 835-841, 2012.
APA
Chae, J., Cho, J.H., Lee, K., Choi, N., Seo, K.S., Kim, S. ... Shim, J. (2012). Role of transcription factor Sp1 in the quercetin-mediated inhibitory effect on human malignant pleural mesothelioma. International Journal of Molecular Medicine, 30, 835-841. https://doi.org/10.3892/ijmm.2012.1075
MLA
Chae, J., Cho, J. H., Lee, K., Choi, N., Seo, K. S., Kim, S., Lee, S., Shim, J."Role of transcription factor Sp1 in the quercetin-mediated inhibitory effect on human malignant pleural mesothelioma". International Journal of Molecular Medicine 30.4 (2012): 835-841.
Chicago
Chae, J., Cho, J. H., Lee, K., Choi, N., Seo, K. S., Kim, S., Lee, S., Shim, J."Role of transcription factor Sp1 in the quercetin-mediated inhibitory effect on human malignant pleural mesothelioma". International Journal of Molecular Medicine 30, no. 4 (2012): 835-841. https://doi.org/10.3892/ijmm.2012.1075
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