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International Journal of Molecular Medicine
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October 2012 Volume 30 Issue 4

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Article

Icariin promotes bone formation via the BMP-2/Smad4 signal transduction pathway in the hFOB 1.19 human osteoblastic cell line

  • Authors:
    • Wenna Liang
    • Munan Lin
    • Xihai Li
    • Candong Li
    • Bizheng Gao
    • Huijuan Gan
    • Zhaoyang Yang
    • Xuejuan Lin
    • Linghong Liao
    • Min Yang
  • View Affiliations / Copyright

    Affiliations: Research Base of Traditional Chinese Medicine Syndrome, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, P.R. China, Department of Traditional Chinese Medicine, Fuzhou General Hospital of Nanjing Military Command, Fuzhou 350025, P.R. China, Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, P.R. China, Research Base of Traditional Chinese Medicine Syndrome, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, P.R. China
  • Pages: 889-895
    |
    Published online on: July 26, 2012
       https://doi.org/10.3892/ijmm.2012.1079
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Abstract

Icariin, the main active compound of the traditional Chinese medicine, Epimedium, is commonly used for the clinical treatment of osteoporosis. However, the precise molecular mechanism of the therapeutic effect of icariin has not been elucidated. The aim of this study was to examine the effect of icariin on cell viability, alkaline phosphatase (ALP) activity, the amount of calcified nodules, and to delineate the molecular mechanism of icariin-enhanced bone formation by investigating the expression of bone morphogenic protein‑2 (BMP-2), Smad4, Cbfa1/Runx2, osteoprotegerin (OPG), receptor activator of nuclear factor κ-B ligand (RANKL) and the OPG/RANKL ratio in the hFOB 1.19 human osteoblastic cell line. We found that icariin significantly increased the cell viability, the activity of ALP and the amount of calcified nodules in the hFOB 1.19 cells. Furthermore, we observed that icariin upregulated the expression of BMP-2, Smad4, Cbfa1/Runx2, OPG, RANKL and the OPG/RANKL ratio. Our results indicate that icariin can modulate the process of bone formation via the BMP-2/Smad4 signal transduction pathway in hFOB 1.19 cells.
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1. 

D NarayananA AnithaR JayakumarSV NairKP ChennazhiSynthesis, characterization and preliminary in vitro evaluation of PTH 1–34 loaded chitosan nanoparticles for osteoporosisJ Biomed Nanotechnol8981062012

2. 

J PrzedlackiStrontium ranelate in post-menopausal osteoporosisEndokrynol Pol626572201121365582

3. 

G PadovaG BorzìL IncorvaiaPrevalence of osteoporosis and vertebral fractures in acromegalic patientsClin Cases Miner Bone Metab83743201122461828

4. 

NS DattaOsteoporotic fracture and parathyroid hormoneWorld J Orthop26774201110.5312/wjo.v2.i8.6722474638

5. 

G QianX ZhangL LuX WuS LiJ MengRegulation of Cbfa1 expression by total flavonoids of Herba epimediiEndocr J538794200610.1507/endocrj.53.8716543677

6. 

L QinT HanQ ZhangAntiosteoporotic chemical constituents from Er-Xian Decoction, a traditional Chinese herbal formulaJ Ethnopharmacol118271279200810.1016/j.jep.2008.04.00918501540

7. 

KM ChenHP MaBF GeIcariin enhances the osteogenic differentiation of bone marrow stromal cells but has no effects on the differentiation of newborn calvarial osteoblasts of ratsPharmazie627857892007

8. 

J HuangL YuanX WangTL ZhangK WangIcaritin and its glycosides enhance osteoblastic, but suppress osteoclastic, differentiation and activity in vitroLife Sci81832840200710.1016/j.lfs.2007.07.01517764702

9. 

SK MokWF ChenWP LaiIcariin protects against bone loss induced by oestrogen deficiency and activates oestrogen receptor-dependent osteoblastic functions in UMR 106 cellsBr J Pharmacol159939949201010.1111/j.1476-5381.2009.00593.x20128811

10. 

HP MaLG MingBF GeIcariin is more potent than genistein in promoting osteoblast differentiation and mineralization in vitroJ Cell Biochem112916923201110.1002/jcb.2300721328465

11. 

J ZhangY LiJ SunC LiuD ZhangSynergistic or antagonistic effect of MTE plus TF or icariin from Epimedium koreanum on the proliferation and differentiation of primary osteoblasts in vitroBiol Trace Elem Res14317461757201110.1007/s12011-011-8987-z21301987

12. 

J XiongCA O’BrienOsteocyte RANKL: new insights into the control of bone remodelingJ Bone Miner Res27499505201210.1002/jbmr.154722354849

13. 

SJ MlakarJ PrezeljJ MarcTesting GSTP1 genotypes and haplotypes interactions in Slovenian post-/pre-menopausal women: novel involvement of glutathione S-transferases in bone remodeling processMaturitas71180187201210.1016/j.maturitas.2011.11.02322221655

14. 

S SchmidtTM PostLA PeletierMA BoroujerdiM DanhofCoping with time scales in disease systems analysis: application to bone remodelingJ Pharmacokinet Pharmacodyn38873900201110.1007/s10928-011-9224-222028207

15. 

H CaoY KeY ZhangCJ ZhangW QianGL ZhangIcariin stimulates MC3T3-E1 cell proliferation and differentiation through up-regulation of bone morphogenetic protein-2Int J Mol Med29435439201222109711

16. 

T MatsubaraK KidaA YamaguchiBMP2 regulates Osterix through Msx2 and Runx2 during osteoblast differentiationJ Biol Chem2832911929125200810.1074/jbc.M80177420018703512

17. 

TP HsiehSY SheuJS SunMH ChenMH LiuIcariin isolated from Epimedium pubescens regulates osteoblasts anabolism through BMP-2, SMAD4, and Cbfa1 expressionPhytomedicine174144232010

18. 

G SwarnkarK SharanJA SiddiquiA novel flavonoid isolated from the steam-bark of Ulmus wallichiana planchon stimulates osteoblast function and inhibits osteoclast and adipocyte differentiationEur J Pharmacol6586573201121376034

19. 

M TabuchiK MiyazawaM KimuraEnhancement of crude bone morphogenetic protein-induced new bone formation and normalization of endochondral ossification by bisphosphonate treatment in osteoprotegerin-deficient miceCalcif Tissue Int77239249200510.1007/s00223-004-0223-9

20. 

S PengG ZhangY HeEpimedium-derived flavonoids promote osteoblastogenesis and suppress adipogenesis in bone marrow stromal cells while exerting an anabolic effect on osteoporotic boneBone45534544200910.1016/j.bone.2009.09.004

21. 

TP HsiehSY SheuJS SunMH ChenIcariin inhibits osteoclast differentiation and bone resorption by suppression of MAPKs/NF-κB regulated HIF-1α and PGE(2) synthesisPhytomedicine18176185201120554188

22. 

J NojimaK KanomataY TakadaDual roles of smad proteins in the conversion from myoblasts to osteoblastic cells by bone morphogenetic proteinsJ Biol Chem2851557715586201010.1074/jbc.M109.02801920231279

23. 

JE Sotillo RodriguezKC ManskyED JensenEnhanced osteoclastogenesis causes osteopenia in twisted gastrulation-deficient mice through increased BMP signalingJ Bone Miner Res2419171926200919419314

24. 

L WangX ZhangY GuoInvolvement of BMPs/Smad signaling pathway in mechanical response in osteoblastsCell Physiol Biochem2610931102201010.1159/00032398721220940

25. 

J ZhaoS OhbaM ShinkaiUI ChungT NagamuneIcariin induces osteogenic differentiation in vitro in a BMP- and Runx2-dependent mannerBiochem Biophys Res Commun369444448200810.1016/j.bbrc.2008.02.05418295595

26. 

Q XiaoA ChenF GuoEffects of Icariin on expression of OPN mRNA and type I collagen in rat osteoblasts in vitroJ Huazhong Univ Sci Technolog Med Sci25690692200510.1007/BF0289617216696327

27. 

B MaQ ZhangD WuStrontium fructose 1,6-diphosphate prevents bone loss in a rat model of postmenopausal osteoporosis via the OPG/RANKL/RANK pathwayActa Pharmacol Sin33479489201210.1038/aps.2011.17722426695

28. 

JW LeeA IwahashiS HasegawaCoptisine inhibits RANKL-induced NF-κB phosphorylation in osteoclast precursors and suppresses function through the regulation of RANKL and OPG gene expression in osteoblastic cellsJ Nat Med66816201221656335

29. 

A EnjuanesS Ruiz-GaspàP PerisThe effect of the alendronate on OPG/RANKL system in differentiated primary human osteoblastsEndocrine37180186201010.1007/s12020-009-9285-920963568

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Copy and paste a formatted citation
Spandidos Publications style
Liang W, Lin M, Li X, Li C, Gao B, Gan H, Yang Z, Lin X, Liao L, Yang M, Yang M, et al: Icariin promotes bone formation via the BMP-2/Smad4 signal transduction pathway in the hFOB 1.19 human osteoblastic cell line. Int J Mol Med 30: 889-895, 2012.
APA
Liang, W., Lin, M., Li, X., Li, C., Gao, B., Gan, H. ... Yang, M. (2012). Icariin promotes bone formation via the BMP-2/Smad4 signal transduction pathway in the hFOB 1.19 human osteoblastic cell line. International Journal of Molecular Medicine, 30, 889-895. https://doi.org/10.3892/ijmm.2012.1079
MLA
Liang, W., Lin, M., Li, X., Li, C., Gao, B., Gan, H., Yang, Z., Lin, X., Liao, L., Yang, M."Icariin promotes bone formation via the BMP-2/Smad4 signal transduction pathway in the hFOB 1.19 human osteoblastic cell line". International Journal of Molecular Medicine 30.4 (2012): 889-895.
Chicago
Liang, W., Lin, M., Li, X., Li, C., Gao, B., Gan, H., Yang, Z., Lin, X., Liao, L., Yang, M."Icariin promotes bone formation via the BMP-2/Smad4 signal transduction pathway in the hFOB 1.19 human osteoblastic cell line". International Journal of Molecular Medicine 30, no. 4 (2012): 889-895. https://doi.org/10.3892/ijmm.2012.1079
Copy and paste a formatted citation
x
Spandidos Publications style
Liang W, Lin M, Li X, Li C, Gao B, Gan H, Yang Z, Lin X, Liao L, Yang M, Yang M, et al: Icariin promotes bone formation via the BMP-2/Smad4 signal transduction pathway in the hFOB 1.19 human osteoblastic cell line. Int J Mol Med 30: 889-895, 2012.
APA
Liang, W., Lin, M., Li, X., Li, C., Gao, B., Gan, H. ... Yang, M. (2012). Icariin promotes bone formation via the BMP-2/Smad4 signal transduction pathway in the hFOB 1.19 human osteoblastic cell line. International Journal of Molecular Medicine, 30, 889-895. https://doi.org/10.3892/ijmm.2012.1079
MLA
Liang, W., Lin, M., Li, X., Li, C., Gao, B., Gan, H., Yang, Z., Lin, X., Liao, L., Yang, M."Icariin promotes bone formation via the BMP-2/Smad4 signal transduction pathway in the hFOB 1.19 human osteoblastic cell line". International Journal of Molecular Medicine 30.4 (2012): 889-895.
Chicago
Liang, W., Lin, M., Li, X., Li, C., Gao, B., Gan, H., Yang, Z., Lin, X., Liao, L., Yang, M."Icariin promotes bone formation via the BMP-2/Smad4 signal transduction pathway in the hFOB 1.19 human osteoblastic cell line". International Journal of Molecular Medicine 30, no. 4 (2012): 889-895. https://doi.org/10.3892/ijmm.2012.1079
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