Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
International Journal of Molecular Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1107-3756 Online ISSN: 1791-244X
Journal Cover
November 2012 Volume 30 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
November 2012 Volume 30 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

ERG is specifically associated with ETS-2 and ETV-4, but not with ETS-1, in prostate cancer

  • Authors:
    • David Adler
    • Jacqueline Ochsenfahrt
    • Kerstin Fuchs
    • Glen Kristiansen
    • Sven Perner
    • Nicolas Wernert
  • View Affiliations / Copyright

    Affiliations: Institute of Pathology, University Hospital of Bonn, D-53127 Bonn, Germany, Department of Prostate Cancer Research, Institute of Pathology, University Hospital of Bonn, D-53127 Bonn, Germany
    Copyright: © Adler et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 1029-1033
    |
    Published online on: August 20, 2012
       https://doi.org/10.3892/ijmm.2012.1097
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The erythroblast transformation-specific (ETS) family of transcription factors plays important roles in both physiological and pathological conditions. Even though many studies have focused on single ETS factors within a single tissue and within the context of specific promoters, the functional impact of multiple ETS members present within a specific cell type has not yet been investigated, especially in prostate cancer (PCa). As the most prominent gene rearrangement in PCa leads to the overexpression of the ETS-related gene (ERG), the aim of this study was to investigate whether ERG is part of a complex integrated transcriptional network that involves other ETS factors. More specifically, as the ETS family consists of 27 members, we focused our efforts initially on investigating whether ERG is associated with the three family members, ETS-1, ETS-2 and ETS variant gene‑4 (ETV‑4), in PCa as a proof of principle. Using western blot analysis, we show that ERG, ETS-1, ETS-2 and ETV-4 are expressed in PC3 cell nuclear extracts and in protein lysates prepared from human PCa prostatectomy specimens. Immunoprecipitations using an anti-ERG antibody were used with PC3 cell nuclear extracts as well as with a pooled protein lysate sample prepared from the PCa tissue samples of five patients. Importantly, our results revealed that ERG is specifically associated with ETS-2 and ETV-4, but not with ETS-1, in PC3 cell nuclear extracts and PCa tissue protein lysates. Our findings strongly support the notion that ERG is part of a complex integrated transcriptional network that involves other ETS factors, which are likely to cooperate or influence the activity of ERG in PCa. The functional impact of multiple ETS factors being associated with ERG in PCa requires further study, as it may provide insights into the mechanism by which ERG exerts its influence in PCa and may subsequently contribute to our understanding of the molecular basis of PCa.
View Figures

Figure 1

Figure 2

Figure 3

View References

1. 

A JemalR SiegelJ XuE WardCancer statistics, 2010CA Cancer J Clin60277300201010.3322/caac.20073

2. 

SA TomlinsDR RhodesS PernerRecurrent fusion of TMPRSS2 and ETS transcription factor genes in prostate cancerScience310644648200510.1126/science.111767916254181

3. 

MA RubinCA MaherAM ChinnaiyanCommon gene rearrangements in prostate cancerJ Clin Oncol2936593668201110.1200/JCO.2011.35.191621859993

4. 

C Kumar-SinhaSA TomlinsAM ChinnaiyanRecurrent gene fusions in prostate cancerNat Rev Cancer8497511200810.1038/nrc240218563191

5. 

PC HollenhorstDA JonesBJ GravesExpression profiles frame the promoter specificity dilemma of the ETS family of transcription factorsNucleic Acids Res3256935702200410.1093/nar/gkh90615498926

6. 

T OikawaETS transcription factors: possible targets for cancer therapyCancer Sci95626633200410.1111/j.1349-7006.2004.tb03320.x15298723

7. 

N HashiyaN JoM AokiIn vivo evidence of angiogenesis induced by transcription factor Ets-1: Ets-1 is located upstream of angiogenesis cascadeCirculation10930353041200410.1161/01.CIR.0000130643.41587.DB15173033

8. 

T RothhammerJC HahneA FlorinThe Ets-1 transcription factor is involved in the development and invasion of malignant melanomaCell Mol Life Sci61118128200410.1007/s00018-003-3337-814704859

9. 

JC HahneAF OkuducuA KaminskiA FlorinF SoncinN WernertEts-1 expression promotes epithelial cell transformation by inducing migration, invasion and anchorage-independent growthOncogene2453845388200510.1038/sj.onc.120876115940256

10. 

A SahinC VercamerA KaminskiDominant-negative inhibition of Ets 1 suppresses tumor growth, invasion and migration in rat C6 glioma cells and reveals differentially expressed Ets 1 target genesInt J Oncol343773892009

11. 

Z ShaikhibrahimB LangerA LindstrotEts-1 is implicated in the regulation of androgen co-regulator FHL2 and reveals specificity for migration, but not invasion, of PC3 prostate cancer cellsOncol Rep2511251129201121258770

12. 

Z ShaikhibrahimA LindstrotB LangerR BuettnerN WernertComprehensive gene expression microarray analysis of Ets-1 blockade in PC3 prostate cancer cells and correlations with prostate cancer tissues: Insights into genes involved in the metastatic cascadeInt J Mol Med278118192011

13. 

Z ShaikhibrahimN WernertETS transcription factors and prostate cancer: The role of the family prototype ETS-1 (Review)Int J Oncol4017481754201222366814

14. 

J DittmerThe biology of the Ets1 proto-oncogeneMol Cancer229200310.1186/1476-4598-2-2912971829

15. 

B WasylykSL HahnA GiovaneThe Ets family of transcription factorsEur J Biochem211718199310.1111/j.1432-1033.1993.tb19864.x8425553

16. 

PC HollenhorstAA ShahC HopkinsBJ GravesGenome-wide analyses reveal properties of redundant and specific promoter occupancy within the ETS gene familyGenes Dev2118821894200710.1101/gad.156170717652178

17. 

G WeiR SrinivasanCZ Cantemir-StoneEts1 and Ets2 are required for endothelial cell survival during embryonic angiogenesisBlood11411231130200910.1182/blood-2009-03-21139119411629

18. 

DK WatsonDP TurnerMN ScheiberVJ FindlayPM WatsonETS transcription factor expression and conversion during prostate and breast cancer progressionOpen Cancer J32439201010.2174/1874079001003010024

19. 

GH WeiG BadisMF BergerGenome-wide analysis of ETS-family DNA-binding in vitro and in vivoEMBO J2921472160201010.1038/emboj.2010.10620517297

20. 

SA TomlinsA BjartellAM ChinnaiyanETS gene fusions in prostate cancer: from discovery to daily clinical practiceEur Urol56275286200910.1016/j.eururo.2009.04.03619409690

21. 

G PetrovicsA LiuS ShaheduzzamanFrequent overexpression of ETS-related gene-1 (ERG1) in prostate cancer transcriptomeOncogene2438473852200510.1038/sj.onc.120851815750627

22. 

K RostadM MannelqvistOJ HalvorsenERG upregulation and related ETS transcription factors in prostate cancerInt J Oncol301932200717143509

23. 

N CerveiraFR RibeiroA PeixotoTMPRSS2-ERG gene fusion causing ERG overexpression precedes chromosome copy number changes in prostate carcinomas and paired HGPIN lesionsNeoplasia8826832200610.1593/neo.0642717032499

24. 

C SunA DobiA MohamedTMPRSS2-ERG fusion, a common genomic alteration in prostate cancer activates C-MYC and abrogates prostate epithelial differentiationOncogene2753485353200810.1038/onc.2008.18318542058

25. 

G AlipovT NakayamaM ItoOverexpression of Ets-1 proto-oncogene in latent and clinical prostatic carcinomasHistopathology46202208200510.1111/j.1365-2559.2005.02059.x15693893

26. 

JP BasuyauxE FerreiraD StehelinG ButticeThe Ets transcription factors interact with each other and with the c-Fos/c-Jun complex via distinct protein domains in a DNA-dependent and -independent mannerJ Biol Chem2722618826195199710.1074/jbc.272.42.261889334186

27. 

MA SvenssonCJ LaFargueTY MacDonaldTesting mutual exclusivity of ETS rearranged prostate cancerLab Invest91404412201110.1038/labinvest.2010.17920975660

28. 

Z ShaikhibrahimA LindstrotJ EllingerS RogenhoferR BuettnerN WernertIdentification of immunity-related genes in prostate cancer and potential role of the ETS family of transcription factors in their regulationInt J Mol Med28799807201121833467

29. 

V EspinaJD WulfkuhleVS CalvertLaser-capture microdissectionNat Protoc1586603200610.1038/nprot.2006.85

30. 

VJ SchebleM BraunR BeroukhimERG rearrangement is specific to prostate cancer and does not occur in any other common tumorMod Pathol2310611067201010.1038/modpathol.2010.8720473283

31. 

JP ClarkCS CooperETS gene fusions in prostate cancerNat Rev Urol6429439200910.1038/nrurol.2009.12719657377

32. 

B HanR MehraK SulemanCharacterization of ETS gene aberrations in select histologic variants of prostate carcinomaMod Pathol2211761185200910.1038/modpathol.2009.7919465903

33. 

CC GuoJY DancerY WangTMPRSS2-ERG gene fusion in small cell carcinoma of the prostateHum Pathol421117201110.1016/j.humpath.2010.05.02621040948

34. 

VJ SchebleM BraunT WilbertzERG rearrangement in small cell prostatic and lung cancerHistopathology56937943201010.1111/j.1365-2559.2010.03564.x20636794

35. 

AM JoshuaA EvansT Van der KwastProstatic preneoplasia and beyondBiochim Biophys Acta1785156181200818166163

36. 

M BraunVJ SchebleR MenonRelevance of cohort design for studying the frequency of the ERG rearrangement in prostate cancerHistopathology5810281036201110.1111/j.1365-2559.2011.03862.x21707704

37. 

VI SementchenkoCW SchweinfestTS PapasDK WatsonETS2 function is required to maintain the transformed state of human prostate cancer cellsOncogene1728832888199810.1038/sj.onc.12022209879994

38. 

GM CarboneS NapoliA ValentiniF CavalliDK WatsonCV CatapanoTriplex DNA-mediated downregulation of Ets2 expression results in growth inhibition and apoptosis in human prostate cancer cellsNucleic Acids Res3243584367200410.1093/nar/gkh74415314206

39. 

Z ShaikhibrahimM BraunP NikolovRearrangement of the ETS genes ETV-1, ETV-4, ETV-5, and ELK-4 is a clonal event during prostate cancer progressionHum PatholMay72012(Epub ahead of print)

40. 

PC HollenhorstL PaulMW FerrisBJ GravesThe ETS gene ETV4 is required for anchorage-independent growth and a cell proliferation gene expression program in PC3 prostate cellsGenes Cancer110441052201110.1177/194760191039557821373373

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Adler D, Ochsenfahrt J, Fuchs K, Kristiansen G, Perner S and Wernert N: ERG is specifically associated with ETS-2 and ETV-4, but not with ETS-1, in prostate cancer. Int J Mol Med 30: 1029-1033, 2012.
APA
Adler, D., Ochsenfahrt, J., Fuchs, K., Kristiansen, G., Perner, S., & Wernert, N. (2012). ERG is specifically associated with ETS-2 and ETV-4, but not with ETS-1, in prostate cancer. International Journal of Molecular Medicine, 30, 1029-1033. https://doi.org/10.3892/ijmm.2012.1097
MLA
Adler, D., Ochsenfahrt, J., Fuchs, K., Kristiansen, G., Perner, S., Wernert, N."ERG is specifically associated with ETS-2 and ETV-4, but not with ETS-1, in prostate cancer". International Journal of Molecular Medicine 30.5 (2012): 1029-1033.
Chicago
Adler, D., Ochsenfahrt, J., Fuchs, K., Kristiansen, G., Perner, S., Wernert, N."ERG is specifically associated with ETS-2 and ETV-4, but not with ETS-1, in prostate cancer". International Journal of Molecular Medicine 30, no. 5 (2012): 1029-1033. https://doi.org/10.3892/ijmm.2012.1097
Copy and paste a formatted citation
x
Spandidos Publications style
Adler D, Ochsenfahrt J, Fuchs K, Kristiansen G, Perner S and Wernert N: ERG is specifically associated with ETS-2 and ETV-4, but not with ETS-1, in prostate cancer. Int J Mol Med 30: 1029-1033, 2012.
APA
Adler, D., Ochsenfahrt, J., Fuchs, K., Kristiansen, G., Perner, S., & Wernert, N. (2012). ERG is specifically associated with ETS-2 and ETV-4, but not with ETS-1, in prostate cancer. International Journal of Molecular Medicine, 30, 1029-1033. https://doi.org/10.3892/ijmm.2012.1097
MLA
Adler, D., Ochsenfahrt, J., Fuchs, K., Kristiansen, G., Perner, S., Wernert, N."ERG is specifically associated with ETS-2 and ETV-4, but not with ETS-1, in prostate cancer". International Journal of Molecular Medicine 30.5 (2012): 1029-1033.
Chicago
Adler, D., Ochsenfahrt, J., Fuchs, K., Kristiansen, G., Perner, S., Wernert, N."ERG is specifically associated with ETS-2 and ETV-4, but not with ETS-1, in prostate cancer". International Journal of Molecular Medicine 30, no. 5 (2012): 1029-1033. https://doi.org/10.3892/ijmm.2012.1097
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team