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Article

HBV promotes the proliferation of hepatic stellate cells via the PDGF-B/PDGFR-β signaling pathway in vitro

  • Authors:
    • Qixuan Bai
    • Jing An
    • Xiaoning Wu
    • Hong You
    • Hong Ma
    • Tianhui Liu
    • Na Gao
    • Jidong Jia
  • View Affiliations / Copyright

    Affiliations: Liver Research Center, Beijing Friendship Hospital, Capital Medical University, Beijing, P.R. China, Department of Microbiology, School of Basic Medical Sciences, Capital Medical University, Beijing, P.R. China
  • Pages: 1443-1450
    |
    Published online on: October 4, 2012
       https://doi.org/10.3892/ijmm.2012.1148
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Abstract

The activation of hepatic stellate cells (HSCs) is closely associated with liver fibrosis in chronic hepatitis B virus (HBV) infection. However, the molecular mechanisms leading to HSC activation remain unclear. It has been reported that the platelet-derived growth factor-B (PDGF-B)/PDGF receptor-β (PDGFR-β) signaling pathway is involved in this process. Thus, we investigated whether HBV and its protein contribute to HSC proliferation by the PDGF-B/PDGFR-β signaling pathway. HBV particles were purified from the supernatant of HepG2.2.15 cells by ultracentrifugation and the cell lines carrying HBV preS, e, c or x genes were obtained. After incubation with HBV particles or co-cultured with the cell lines expressed in the viral protein, the proliferation of LX-2 cells, an HSC cell line, were detected by flow cyto-metry and real-time PCR and the expression of molecules related to the PDGF-B/PDGFR-β signaling pathway were further measured. Our results indicated that HBV particles, c and x proteins promoted LX-2 proliferation and increased the mRNA levels of PDGF-B, PDGFR-β, collagen-I and α-smooth muscle actin (α-SMA), as well as the phosphorylation of PDGFR-β; however, the expression protein levels of PDGF-B and PDGFR-β remained unchanged. In conclusion, HBV particles and HBV c and x proteins promote HSC proliferation and fibrogenesis in vitro and the PDGF-B/PDGFR-β signaling pathway is important in this process.
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Copy and paste a formatted citation
Spandidos Publications style
Bai Q, An J, Wu X, You H, Ma H, Liu T, Gao N and Jia J: HBV promotes the proliferation of hepatic stellate cells via the PDGF-B/PDGFR-β signaling pathway in vitro. Int J Mol Med 30: 1443-1450, 2012.
APA
Bai, Q., An, J., Wu, X., You, H., Ma, H., Liu, T. ... Jia, J. (2012). HBV promotes the proliferation of hepatic stellate cells via the PDGF-B/PDGFR-β signaling pathway in vitro. International Journal of Molecular Medicine, 30, 1443-1450. https://doi.org/10.3892/ijmm.2012.1148
MLA
Bai, Q., An, J., Wu, X., You, H., Ma, H., Liu, T., Gao, N., Jia, J."HBV promotes the proliferation of hepatic stellate cells via the PDGF-B/PDGFR-β signaling pathway in vitro". International Journal of Molecular Medicine 30.6 (2012): 1443-1450.
Chicago
Bai, Q., An, J., Wu, X., You, H., Ma, H., Liu, T., Gao, N., Jia, J."HBV promotes the proliferation of hepatic stellate cells via the PDGF-B/PDGFR-β signaling pathway in vitro". International Journal of Molecular Medicine 30, no. 6 (2012): 1443-1450. https://doi.org/10.3892/ijmm.2012.1148
Copy and paste a formatted citation
x
Spandidos Publications style
Bai Q, An J, Wu X, You H, Ma H, Liu T, Gao N and Jia J: HBV promotes the proliferation of hepatic stellate cells via the PDGF-B/PDGFR-β signaling pathway in vitro. Int J Mol Med 30: 1443-1450, 2012.
APA
Bai, Q., An, J., Wu, X., You, H., Ma, H., Liu, T. ... Jia, J. (2012). HBV promotes the proliferation of hepatic stellate cells via the PDGF-B/PDGFR-β signaling pathway in vitro. International Journal of Molecular Medicine, 30, 1443-1450. https://doi.org/10.3892/ijmm.2012.1148
MLA
Bai, Q., An, J., Wu, X., You, H., Ma, H., Liu, T., Gao, N., Jia, J."HBV promotes the proliferation of hepatic stellate cells via the PDGF-B/PDGFR-β signaling pathway in vitro". International Journal of Molecular Medicine 30.6 (2012): 1443-1450.
Chicago
Bai, Q., An, J., Wu, X., You, H., Ma, H., Liu, T., Gao, N., Jia, J."HBV promotes the proliferation of hepatic stellate cells via the PDGF-B/PDGFR-β signaling pathway in vitro". International Journal of Molecular Medicine 30, no. 6 (2012): 1443-1450. https://doi.org/10.3892/ijmm.2012.1148
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