Arginine inactivates human herpesvirus 2 and inhibits genital herpesvirus infection

Ikeda,Keiko; Yamasaki,Hisashi; Minami,Sawako; Suzuki,Yukiko; Tsujimoto,Kazuko; Sekino,Yoshihisa; Irie,Hiroshi; Arakawa,Tsutomu; Koyama,A.  Hajime

doi:10.3892/ijmm.2012.1149

December 2012 Volume 30 Issue 6

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December 2012 Volume 30 Issue 6

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Article

Arginine inactivates human herpesvirus 2 and inhibits genital herpesvirus infection

Authors:
- Keiko Ikeda
- Hisashi Yamasaki
- Sawako Minami
- Yukiko Suzuki
- Kazuko Tsujimoto
- Yoshihisa Sekino
- Hiroshi Irie
- Tsutomu Arakawa
- A. Hajime Koyama
View Affiliations / Copyright

Affiliations: Department of Cellular and Molecular Medicine, Division of Virology, Wakayama Medical University Graduate School of Medicine, Wakayama 641-8509, Japan, Department of Reproductive and Developmental Medicine, Division of Reproductive Medicine, Wakayama Medical University Graduate School of Medicine, Wakayama 641-8509, Japan, School of Health and Nursing Science, Wakayama Medical University, Wakayama 641-8509, Japan, Wakayama Shin-ai Women's Junior College, Wakayama 640-0341, Japan, Department of Anatomy, School of Medicine, Teikyo University, Itabashi 173-8605, Japan, Department of Sport and Medical Science, School of Medical Technology, Teikyo University, Itabashi 173-8605, Japan, Alliance Protein Laboratories, Thousand Oaks, CA 91360, USA
Pages: 1307-1312
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Published online on: October 4, 2012

https://doi.org/10.3892/ijmm.2012.1149
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Abstract

Arginine, among the amino acids, has demonstrated unique properties, including suppression of protein-protein interactions and virus inactivation. We investigated the effects of arginine on the infectivity of human herpesvirus 2 (HHV-2) and the potential application of arginine as a chemotherapeutic agent against genital herpes. Arginine directly inactivated HHV-2 and characterization of the inactivation demonstrated that 1 M arginine at pH 4.3 inactivated the virus more efficiently compared to 0.1 M citrate or 1 M sodium chloride, indicating that neither acidic pH nor ionic strength alone is sufficient for virus inactivation. The effect of arginine was rapid and concentration-dependent. Although virus inactivation was efficient at an acidic pH, arginine inactivated the virus even at a neutral pH, provided that a higher arginine concentration and prolonged incubation time were used. In addition, arginine suppressed the multiplication of HHV-2 under the conditions at which its effect on cell viability was insignificant. Pilot mouse model studies revealed a marked suppression of death by arginine when the mice were infected with HHV-2 through the vaginal route, followed by an intermittent application of acidic arginine by vaginal instillation.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Arginine inactivates human herpesvirus 2 and inhibits genital herpesvirus infection

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