Short-term mechanical stress inhibits osteoclastogenesis via suppression of DC-STAMP in RAW264.7 cells

  • Authors:
    • Sumika Kameyama
    • Yoshitaka Yoshimura
    • Takeshi Kameyama
    • Takashi Kikuiri
    • Mino Matsuno
    • Yoshiaki Deyama
    • Kuniaki Suzuki
    • Junichiro Iida
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  • Published online on: December 21, 2012     https://doi.org/10.3892/ijmm.2012.1220
  • Pages: 292-298
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Abstract

Mechanical stress is an important factor in bone homeostasis, which is maintained by a balance between bone resorption by osteoclasts and bone formation by osteoblasts. However, little is known about the effects of mechanical stress on osteoclast differentiation. In this study, we examined the effects of short-term mechanical stress on osteoclastogenesis by applying tensile force to RAW264.7 cells stimulated with receptor activator of nuclear factor-κB ligand (RANKL) using a Flexercell tension system. We counted the number of osteoclasts that were tartrate-resistant acid phosphatase (TRAP)-positive and multinucleated (two or more nuclei) with or without application of mechanical stress for 24 h. Osteoclast number was lower after mechanical stress compared with no mechanical stress. Furthermore, mechanical stress for up to 24 h caused downregulation of osteoclast-specific gene expression and fusion-related molecule [dendritic cell specific transmembrane protein (DC-STAMP), osteoclast stimulatory transmembrane protein (OC-STAMP), E-cadherin, Integrin αV and Integrin β3] mRNA levels. Protein expression of DC-STAMP decreased with mechanical stress for 24 h compared to the control without mechanical stress, whereas the expression of E-cadherin, Integrin αV and Integrin β3 was slightly decreased. Nuclear factor of activated T cells c1 (NFATc1) mRNA levels were decreased at 6 h and increased at 12 and 24 h compared with the control. The levels of NFATc2, NFATc3 mRNA did not change compared with the control group. By contrast, mechanical stress for 24 h significantly enhanced NFAT transcriptional activity compared with the control, despite a decrease in DC-STAMP mRNA and protein levels. These results suggest that short-term mechanical stress strongly inhibits osteoclastogenesis through the downregulation of DC-STAMP and other fusion-related molecules and that short-term mechanical stress induces a negative regulatory mechanism that cancels the enhancement of NFAT transcriptional activity.
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February 2013
Volume 31 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Kameyama S, Yoshimura Y, Kameyama T, Kikuiri T, Matsuno M, Deyama Y, Suzuki K and Iida J: Short-term mechanical stress inhibits osteoclastogenesis via suppression of DC-STAMP in RAW264.7 cells. Int J Mol Med 31: 292-298, 2013
APA
Kameyama, S., Yoshimura, Y., Kameyama, T., Kikuiri, T., Matsuno, M., Deyama, Y. ... Iida, J. (2013). Short-term mechanical stress inhibits osteoclastogenesis via suppression of DC-STAMP in RAW264.7 cells. International Journal of Molecular Medicine, 31, 292-298. https://doi.org/10.3892/ijmm.2012.1220
MLA
Kameyama, S., Yoshimura, Y., Kameyama, T., Kikuiri, T., Matsuno, M., Deyama, Y., Suzuki, K., Iida, J."Short-term mechanical stress inhibits osteoclastogenesis via suppression of DC-STAMP in RAW264.7 cells". International Journal of Molecular Medicine 31.2 (2013): 292-298.
Chicago
Kameyama, S., Yoshimura, Y., Kameyama, T., Kikuiri, T., Matsuno, M., Deyama, Y., Suzuki, K., Iida, J."Short-term mechanical stress inhibits osteoclastogenesis via suppression of DC-STAMP in RAW264.7 cells". International Journal of Molecular Medicine 31, no. 2 (2013): 292-298. https://doi.org/10.3892/ijmm.2012.1220