Soybean glyceollins mitigate inducible nitric oxide synthase and cyclooxygenase-2 expression levels via suppression of the NF-κB signaling pathway in RAW 264.7 cells

  • Authors:
    • Eun-Kyung Yoon
    • Hyun-Kyoung Kim
    • Song Cui
    • Yong-Hoon Kim
    • Sang-Han Lee
  • View Affiliations

  • Published online on: January 12, 2012     https://doi.org/10.3892/ijmm.2012.887
  • Pages: 711-717
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Abstract

Glyceollins, produced to induce disease resistance responses against specific species, such as an incompatible pathogen Phytophthora sojae in soybeans, have the potential to exhibit anti-inflammatory activity in RAW 264.7 cells. To investigate the anti-inflammatory effects of elicited glyceollins via a signaling pathway, we studied the glyceollin signaling pathway using several assays including RNA and protein expression levels. We found that soybean glyceollins significantly reduced LPS-induced nitric oxide (NO) and prostaglandin E2 (PGE2) production, as well as the expression of inducible ΝΟ synthase (iNOS) and cyclooxygenase-2 (COX-2) via the suppression of NF-κB activation. Glyceollins also inhibited the phosphorylation of IκBα kinase (IKK), the degradation of IκBα, and the formation of NF-κB-DNA binding complex in a dose-dependent manner. Furthermore, they inhibited pro-inflammatory cytokines, such as tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-18, but increased the generation of the anti-inflammatory cytokine IL-10. Collectively, the present data show that glyceollins elicit potential anti-inflammatory effects by suppressing the NF-κB signaling pathway in RAW 264.7 cells.
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April 2012
Volume 29 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Yoon E, Kim H, Cui S, Kim Y and Lee S: Soybean glyceollins mitigate inducible nitric oxide synthase and cyclooxygenase-2 expression levels via suppression of the NF-κB signaling pathway in RAW 264.7 cells. Int J Mol Med 29: 711-717, 2012
APA
Yoon, E., Kim, H., Cui, S., Kim, Y., & Lee, S. (2012). Soybean glyceollins mitigate inducible nitric oxide synthase and cyclooxygenase-2 expression levels via suppression of the NF-κB signaling pathway in RAW 264.7 cells. International Journal of Molecular Medicine, 29, 711-717. https://doi.org/10.3892/ijmm.2012.887
MLA
Yoon, E., Kim, H., Cui, S., Kim, Y., Lee, S."Soybean glyceollins mitigate inducible nitric oxide synthase and cyclooxygenase-2 expression levels via suppression of the NF-κB signaling pathway in RAW 264.7 cells". International Journal of Molecular Medicine 29.4 (2012): 711-717.
Chicago
Yoon, E., Kim, H., Cui, S., Kim, Y., Lee, S."Soybean glyceollins mitigate inducible nitric oxide synthase and cyclooxygenase-2 expression levels via suppression of the NF-κB signaling pathway in RAW 264.7 cells". International Journal of Molecular Medicine 29, no. 4 (2012): 711-717. https://doi.org/10.3892/ijmm.2012.887