Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
International Journal of Molecular Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1107-3756 Online ISSN: 1791-244X
Journal Cover
April 2012 Volume 29 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
April 2012 Volume 29 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Soybean glyceollins mitigate inducible nitric oxide synthase and cyclooxygenase-2 expression levels via suppression of the NF-κB signaling pathway in RAW 264.7 cells

  • Authors:
    • Eun-Kyung Yoon
    • Hyun-Kyoung Kim
    • Song Cui
    • Yong-Hoon Kim
    • Sang-Han Lee
  • View Affiliations / Copyright

    Affiliations: Department of Food Science and Biotechnology, Kyungpook National University, Daegu 702-701, Republic of Korea
  • Pages: 711-717
    |
    Published online on: January 12, 2012
       https://doi.org/10.3892/ijmm.2012.887
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Glyceollins, produced to induce disease resistance responses against specific species, such as an incompatible pathogen Phytophthora sojae in soybeans, have the potential to exhibit anti-inflammatory activity in RAW 264.7 cells. To investigate the anti-inflammatory effects of elicited glyceollins via a signaling pathway, we studied the glyceollin signaling pathway using several assays including RNA and protein expression levels. We found that soybean glyceollins significantly reduced LPS-induced nitric oxide (NO) and prostaglandin E2 (PGE2) production, as well as the expression of inducible ΝΟ synthase (iNOS) and cyclooxygenase-2 (COX-2) via the suppression of NF-κB activation. Glyceollins also inhibited the phosphorylation of IκBα kinase (IKK), the degradation of IκBα, and the formation of NF-κB-DNA binding complex in a dose-dependent manner. Furthermore, they inhibited pro-inflammatory cytokines, such as tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-18, but increased the generation of the anti-inflammatory cytokine IL-10. Collectively, the present data show that glyceollins elicit potential anti-inflammatory effects by suppressing the NF-κB signaling pathway in RAW 264.7 cells.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

N TorresI Torre-VillalvazoAR TovarRegulation of lipid metabolism by soy protein and its implication in diseases mediated by lipid disordersJ Nutr Biochem17365373200610.1016/j.jnutbio.2005.11.00516481155

2 

TB NgXJ YeJH WongEF FangYS ChanW PanXY YeSC SzeKY ZhangF LiuHX WangGlyceollin, a soybean phytoalexin with medicinal propertiesAppl Microbiol Biotechnol5890599068201121336922

3 

WO SongOK ChunI HwangHS ShinBG KimKS KimSY LeeD ShinSG LeeSoy isoflavones as safe functional ingredientsJ Med Food10571580200710.1089/jmf.2006.062018158825

4 

CD AllredKF AllredYH JuTS GeoppingerDR DoergeWG HelferichSoy processing influences growth of estrogen dependent breast cancer tumorsCarcinogenesis2516491657200510.1093/carcin/bgh17815131010

5 

M MessinaSoy foods and soybean phytoestrogens (isoflavones) as possible alternatives to hormone replacement therapy (HRT)Eur J Cancer367177200010.1016/S0959-8049(00)00233-111056326

6 

DS LeeSH LeeGenistein, a soy isoflavone, is a potent alpha-glucosidase inhibitorFEBS Lett5018486200110.1016/S0014-5793(01)02631-X11457461

7 

P JeandetAC Douillet-BreuilR BessisS DebordM SbaghiM AdrianPhytoalexins from the Vitaceae: biosynthesis, phytoalexin gene expression in transgenic plants, antifungal activity, and metabolismJ Agric Food Chem5027312741200210.1021/jf011429s11982391

8 

SM BouéCH CarterKC EhrlichTE ClevelandInduction of the soybean phytoalexins coumestrol and glyceollin by AspergillusJ Agric Food Chem82167172200010888516

9 

ME BurowSM BouéBM Collins-BurowLI MelnikBN DuongCH Carter-WientjesS LiTE WieseTE ClevelandJA McLachlanPhytochemical glyceollins, isolated from soy, mediate antihormonal effects through estrogen receptor α and βJ Clin Endocrinol Metab8617501758200111297613

10 

GN NikovNE HopkinsS BoueWL AlworthInteractions of dietary estrogens with human estrogen receptors and the effect on estrogen receptor-estrogen response element complex formationEnviron Health Perspect108867872200010.1289/ehp.0010886711017892

11 

HJ KimHJ SuhJH KimS ParkYC JooJS KimAntioxidant activity of glyceollins derived from soybean elicited with Aspergillus sojaeJ Agric Food Chem581163311638201010.1021/jf102829z21033668

12 

VA SalvoSM BouéJP FonsecaS ElliottC CorbittBM Collins-BurowTJ CurielSK SrivastavBY ShihCC WientjesAntiestrogenic glyceollins suppress human breast and ovarian carcinoma tumorigenesisClin Cancer Res1271597164200610.1158/1078-0432.CCR-06-142617145841

13 

CE WoodTB ClarksonSE ApptAA FrankeSM BouéME BurowT McCoyJM ClineEffects of soybean glyceollins and estradiol in postmenopausal female monkeysNutr Cancer567481200610.1207/s15327914nc5601_1017176220

14 

T HenkelU ZabelE FanningPA BaeuerleIntramolecular masking of the nuclear location signal and dimerization domain in the precursor for the p50 NF-κB subunitCell681121113319921547506

15 

M KarinM DelhaseThe IκB kinase (IKK) and NF-κB: key elements of proinflammatory signalingSemin Immunol1285982000

16 

S GhoshM KarinMissing pieces in the NF-κB puzzleCell109SupplS81S962002

17 

MS HaydenS GhoshSignaling to NF-κBGenes Develop18219522242004

18 

E ZandiM KarinBridging the gap composition, regulation and physiological function of the IκB kinase complexMol Cell Biol1945474551199910373503

19 

T WangX ZhangJJ LiThe role of NF-κB in the regulation of cell stress responsesInt Immunopharmacol2150915202002

20 

F YangE TangK GuanCY WangIKK plays an essential role in the phosphorylation of RelA/p65 on serine 536 induced by lipopolysaccharideJ Immunol17056305635200310.4049/jimmunol.170.11.563012759443

21 

HL PahlActivators and target genes of Rel/NF-κB transcription factorsOncogene18685368661999

22 

JT WuJG KralThe NF-κB/IκB signaling system: a molecular target in breast cancer therapyJ Surg Res1231581692005

23 

R LangRL RutschmanDR GreavesPJ MurrayAutocrine deactivation of macrophages in transgenic mice constitutively overexpressing IL-10 under control of the human CD68 promoterJ Immunol16834023411200210.4049/jimmunol.168.7.340211907098

24 

LL CheshireAS Baldwin JrSynergistic activation of NF-κB by tumor necrosis factor α and interferon via enhanced IκBα degradation and de novo IκBα degradationMol Cell Biol17674667541997

25 

J KlostergaardA rapid extremely sensitive, quantitative microassay for cytotoxic cytokinesLymphokine Res430931719853876491

26 

CJ LowensteinEW AlleyP RavalAM SnowmanSH SnyderSW RussellWJ MurphyMacrophage nitric oxide synthase gene: two upstream regions mediate induction by interferon gamma and lipopolysaccharideProc Natl Acad Sci USA9097309734199310.1073/pnas.90.20.97307692452

27 

CH ParkDY NamHU SonSR LeeHJ LeeJC HeoTY ChaJH BaekSH LeePolymer fraction of Aloe vera exhibits a protective activity on ethanol-induced gastric lesionsInt J Mol Med275115182011

28 

MH PanYH ChangML TsaiCS LaiSY HoV BadmaevCT HoPterostilbene suppressed lipopolysaccharide-induced up-expression of iNOS and COX-2 in murine macrophagesJ Agric Food Chem5675027509200810.1021/jf800820y18656926

29 

N KusunokiK KitaharaF KojimaN TanakaK KanekoH EndoT SuguroS KawaiAdiponectin stimulates prostaglandin E(2) production in rheumatoid arthritis synovial fibroblastsArthritis Rheum6216411649201010.1002/art.2745020222108

30 

KS AhnEJ NohHL ZhaoSH JungSS KangYS KimInhibition of inducible nitric oxide synthase and cyclooxygenase II by Platycodon grandiflorum saponins via suppression of nuclear factor-κB activation in RAW 264.7 cellsLife Sci7623152328200510.1016/j.lfs.2004.10.04215748625

31 

MM BradfordA rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnal Biochem72248254197610.1016/0003-2697(76)90527-3942051

32 

I AndújarMC RecioT BacelliRM GinerJL RíosShikonin reduces oedema induced by phorbol ester by interfering with IκBα degradation thus inhibiting translocation of NF-κB to the nucleusBr J Pharmacol160376388201020423347

33 

M SonA KimJ LeeCH ParkJC HeoHJ LeeSH LeeEthanol extract of Lycoris radiata induces cell death in B16F10 melanoma via p38-mediated AP-1 activationOncol Rep244734782010

34 

JD PaxtonBiosynthesis and accumulation of legume phytoalexinsMycotoxins and PhytoalexinsRP SharmaDK SalunkheCRC PressBoca Raton4854991991

35 

LI Rivera-VargaAF SchmitthennerTL GrahamSoybean flavonoid effects on metabolism by Phytophthora sojaePhytochemistry32851857199310.1016/0031-9422(93)85219-H

36 

S FengCL SawYK LeeD HuangFungal-stressed germination of black soybeans leads to generation of oxooctadecadienoic acids in addition to glyceollinsJ Agric Food Chem5585898595200710.1021/jf071673517892258

37 

E AndreakosTargeting cytokines in autoimmunity: new approaches, new promiseExpert Opin Biol Ther3435447200310.1517/14712598.3.3.43512783612

38 

F ChenLM DemersX ShiUpstream signal transduction of NF-κB activationCurr Drug Targets Inflamm Allergy11371492002

39 

D KulmsT SchwarzNF-κB and cytokinesVitam Horm742833002006

40 

K SukS KimU KimRegulation of IL-18 production by IFN gamma and PGE2 in mouse microglial cells: involvement of NF-κB pathway in the regulatory processesImmunol Lett777985200111377701

41 

S MocellinMC PanelliE WangD NagorsenFM MarincolaThe dual role of IL-10Trends Immunol243643200310.1016/S1471-4906(02)00009-1

42 

AT PaulVM GohilKK BhutaniModulating TNF-α signaling with natural productsDrug Discov Today117257322006

43 

DM MinichJS BlandDietary management of the metabolic syndrome beyond macronutrientsNutr Rev66429444200810.1111/j.1753-4887.2008.00075.x18667004

44 

PA RuizD HallerFunctional diversity of flavonoids in the inhibition of the proinflammatory NF-kappaB, IRF, and Akt signaling pathways in murine intestinal epithelial cellsJ Nutr136664671200616484540

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Yoon E, Kim H, Cui S, Kim Y and Lee S: Soybean glyceollins mitigate inducible nitric oxide synthase and cyclooxygenase-2 expression levels via suppression of the NF-κB signaling pathway in RAW 264.7 cells. Int J Mol Med 29: 711-717, 2012.
APA
Yoon, E., Kim, H., Cui, S., Kim, Y., & Lee, S. (2012). Soybean glyceollins mitigate inducible nitric oxide synthase and cyclooxygenase-2 expression levels via suppression of the NF-κB signaling pathway in RAW 264.7 cells. International Journal of Molecular Medicine, 29, 711-717. https://doi.org/10.3892/ijmm.2012.887
MLA
Yoon, E., Kim, H., Cui, S., Kim, Y., Lee, S."Soybean glyceollins mitigate inducible nitric oxide synthase and cyclooxygenase-2 expression levels via suppression of the NF-κB signaling pathway in RAW 264.7 cells". International Journal of Molecular Medicine 29.4 (2012): 711-717.
Chicago
Yoon, E., Kim, H., Cui, S., Kim, Y., Lee, S."Soybean glyceollins mitigate inducible nitric oxide synthase and cyclooxygenase-2 expression levels via suppression of the NF-κB signaling pathway in RAW 264.7 cells". International Journal of Molecular Medicine 29, no. 4 (2012): 711-717. https://doi.org/10.3892/ijmm.2012.887
Copy and paste a formatted citation
x
Spandidos Publications style
Yoon E, Kim H, Cui S, Kim Y and Lee S: Soybean glyceollins mitigate inducible nitric oxide synthase and cyclooxygenase-2 expression levels via suppression of the NF-κB signaling pathway in RAW 264.7 cells. Int J Mol Med 29: 711-717, 2012.
APA
Yoon, E., Kim, H., Cui, S., Kim, Y., & Lee, S. (2012). Soybean glyceollins mitigate inducible nitric oxide synthase and cyclooxygenase-2 expression levels via suppression of the NF-κB signaling pathway in RAW 264.7 cells. International Journal of Molecular Medicine, 29, 711-717. https://doi.org/10.3892/ijmm.2012.887
MLA
Yoon, E., Kim, H., Cui, S., Kim, Y., Lee, S."Soybean glyceollins mitigate inducible nitric oxide synthase and cyclooxygenase-2 expression levels via suppression of the NF-κB signaling pathway in RAW 264.7 cells". International Journal of Molecular Medicine 29.4 (2012): 711-717.
Chicago
Yoon, E., Kim, H., Cui, S., Kim, Y., Lee, S."Soybean glyceollins mitigate inducible nitric oxide synthase and cyclooxygenase-2 expression levels via suppression of the NF-κB signaling pathway in RAW 264.7 cells". International Journal of Molecular Medicine 29, no. 4 (2012): 711-717. https://doi.org/10.3892/ijmm.2012.887
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team