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International Journal of Molecular Medicine
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Print ISSN: 1107-3756 Online ISSN: 1791-244X
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August 2012 Volume 30 Issue 2

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

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Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

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International Journal of Epigenetics

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Article

Adenovirus-mediated TRAIL expression and downregulation of Bcl-2 expression suppresses non-small cell lung cancer growth in vitro and in vivo

  • Authors:
    • Haiping Zhang
    • Aihua Sui
    • Zhenli Wang
    • Shihai Liu
    • Ruyong Yao
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Laboratory, The Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, P.R. China, Central Laboratory, The Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, P.R. China, Department of Pharmacology, The First Sanatorium of Jinan Military District, Qingdao 266001, P.R. China
  • Pages: 358-364
    |
    Published online on: May 14, 2012
       https://doi.org/10.3892/ijmm.2012.998
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Abstract

Primary or acquired resistance to current treatment methods remains a major factor in clinical oncology and may be caused by failures in apoptosis programs. TNF-related apoptosis-inducing ligand (TRAIL) can induce apoptosis in several human cancer cell types. However, not all cancer cells are susceptible to TRAIL and mechanisms of resistance and new strategies to enhance sensitivity are an area of intense investigation. Therefore, we investigated whether TRAIL resistance is due to Bcl-2 levels. In this study, we generated an adenoviral vector, Ad5.TRAIL/siBcl2, that permitted co-expression of shRNA against Bcl-2 and the tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) therapeutic gene from a cytomegalovirus promoter. Infection with Ad5.TRAIL/siBcl2 resulted in significant cytotoxicity in non-small cell lung cancer (NSCLC) cells in vitro. In contrast, it had no effect on a normal lung cell line, WI-38. Impressively, treatment of the established NSCLC tumor model with Ad5.TRAIL/siBcl2 resulted in significant tumor regression, compared with other adenoviruses. This potent antitumor activity induced by Ad5.TRAIL/siBcl2 was due to strong inhibition of Bcl-2 and high expression of TRAIL. Thus, this study may provide a framework for future clinical applications of Ad5.TRAIL/siBcl2 in lung tumor gene therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang H, Sui A, Wang Z, Liu S and Yao R: Adenovirus-mediated TRAIL expression and downregulation of Bcl-2 expression suppresses non-small cell lung cancer growth in vitro and in vivo. Int J Mol Med 30: 358-364, 2012.
APA
Zhang, H., Sui, A., Wang, Z., Liu, S., & Yao, R. (2012). Adenovirus-mediated TRAIL expression and downregulation of Bcl-2 expression suppresses non-small cell lung cancer growth in vitro and in vivo. International Journal of Molecular Medicine, 30, 358-364. https://doi.org/10.3892/ijmm.2012.998
MLA
Zhang, H., Sui, A., Wang, Z., Liu, S., Yao, R."Adenovirus-mediated TRAIL expression and downregulation of Bcl-2 expression suppresses non-small cell lung cancer growth in vitro and in vivo". International Journal of Molecular Medicine 30.2 (2012): 358-364.
Chicago
Zhang, H., Sui, A., Wang, Z., Liu, S., Yao, R."Adenovirus-mediated TRAIL expression and downregulation of Bcl-2 expression suppresses non-small cell lung cancer growth in vitro and in vivo". International Journal of Molecular Medicine 30, no. 2 (2012): 358-364. https://doi.org/10.3892/ijmm.2012.998
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang H, Sui A, Wang Z, Liu S and Yao R: Adenovirus-mediated TRAIL expression and downregulation of Bcl-2 expression suppresses non-small cell lung cancer growth in vitro and in vivo. Int J Mol Med 30: 358-364, 2012.
APA
Zhang, H., Sui, A., Wang, Z., Liu, S., & Yao, R. (2012). Adenovirus-mediated TRAIL expression and downregulation of Bcl-2 expression suppresses non-small cell lung cancer growth in vitro and in vivo. International Journal of Molecular Medicine, 30, 358-364. https://doi.org/10.3892/ijmm.2012.998
MLA
Zhang, H., Sui, A., Wang, Z., Liu, S., Yao, R."Adenovirus-mediated TRAIL expression and downregulation of Bcl-2 expression suppresses non-small cell lung cancer growth in vitro and in vivo". International Journal of Molecular Medicine 30.2 (2012): 358-364.
Chicago
Zhang, H., Sui, A., Wang, Z., Liu, S., Yao, R."Adenovirus-mediated TRAIL expression and downregulation of Bcl-2 expression suppresses non-small cell lung cancer growth in vitro and in vivo". International Journal of Molecular Medicine 30, no. 2 (2012): 358-364. https://doi.org/10.3892/ijmm.2012.998
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