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International Journal of Molecular Medicine
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Article

Osteogenic potential of bone marrow stromal cells derived from streptozotocin-induced diabetic rats

  • Authors:
    • Yan-Fang Zhao
    • De-Liang Zeng
    • Lun-Guo Xia
    • Song-Mei Zhang
    • Lian-Yi Xu
    • Xin-Quan Jiang
    • Fu-Qiang Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Prosthodontics, Ninth People's Hospital affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, P.R. China, Department of Oral and Maxillofacial Surgery, Ninth People's Hospital affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, P.R. China, Oral Bioengineering Laboratory/Regenerative Medicine Laboratory, Shanghai Research Institute of Stomatology, Shanghai Key Laboratory of Stomatology, Ninth People's Hospital affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, P.R. China
  • Pages: 614-620
    |
    Published online on: January 3, 2013
       https://doi.org/10.3892/ijmm.2013.1227
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Abstract

Type 1 diabetes mellitus (T1DM) is associated with a series of bone complications, which are still a great challenge in the clinic. Bone marrow stromal cells (BMSCs) are crucial to bone remodeling and are attractive candidates for tissue engineering. Hence, we aimed to investigate whether impaired functions of BMSCs play a role in the pathogenesis of bone complications associated with T1DM. BMSCs were isolated from normal and streptozotocin-induced diabetic rats, and their proliferation and osteogenic differentiation ability were analyzed. Diabetic BMSCs demonstrated reduced proliferation ability, osteoblast gene expression, alkaline phosphatase activity and mineralization. Nude mice transplanted with diabetic BMSCs in a calcium phosphate cement scaffold exhibited reduced new bone formation, as detected by hematoxylin and eosin staining and immunohistochemistry. These changes may be partially related to impaired insulin and insulin-like growth factor 1 (IGF-1) signaling. Weak gene expression of insulin receptor (IR), IGF-1, insulin-like growth factor 1 receptor (IGF-1R), and insulin receptor substrate-1 (IRS-1) was observed in the diabetic BMSCs compared with normal BMSCs, together with decreased protein level of IGF-1, IGF-1R, IRS-1 and phosphorylated extracellular signal-regulated kinase. Therefore, impaired proliferation and osteogenic potential of BMSCs may be responsible for bone complications related to T1DM, mediated partially by impaired insulin and IGF-1 signaling. These findings may provide a new target with which to devise strategies for therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Zhao Y, Zeng D, Xia L, Zhang S, Xu L, Jiang X and Zhang F: Osteogenic potential of bone marrow stromal cells derived from streptozotocin-induced diabetic rats. Int J Mol Med 31: 614-620, 2013.
APA
Zhao, Y., Zeng, D., Xia, L., Zhang, S., Xu, L., Jiang, X., & Zhang, F. (2013). Osteogenic potential of bone marrow stromal cells derived from streptozotocin-induced diabetic rats. International Journal of Molecular Medicine, 31, 614-620. https://doi.org/10.3892/ijmm.2013.1227
MLA
Zhao, Y., Zeng, D., Xia, L., Zhang, S., Xu, L., Jiang, X., Zhang, F."Osteogenic potential of bone marrow stromal cells derived from streptozotocin-induced diabetic rats". International Journal of Molecular Medicine 31.3 (2013): 614-620.
Chicago
Zhao, Y., Zeng, D., Xia, L., Zhang, S., Xu, L., Jiang, X., Zhang, F."Osteogenic potential of bone marrow stromal cells derived from streptozotocin-induced diabetic rats". International Journal of Molecular Medicine 31, no. 3 (2013): 614-620. https://doi.org/10.3892/ijmm.2013.1227
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao Y, Zeng D, Xia L, Zhang S, Xu L, Jiang X and Zhang F: Osteogenic potential of bone marrow stromal cells derived from streptozotocin-induced diabetic rats. Int J Mol Med 31: 614-620, 2013.
APA
Zhao, Y., Zeng, D., Xia, L., Zhang, S., Xu, L., Jiang, X., & Zhang, F. (2013). Osteogenic potential of bone marrow stromal cells derived from streptozotocin-induced diabetic rats. International Journal of Molecular Medicine, 31, 614-620. https://doi.org/10.3892/ijmm.2013.1227
MLA
Zhao, Y., Zeng, D., Xia, L., Zhang, S., Xu, L., Jiang, X., Zhang, F."Osteogenic potential of bone marrow stromal cells derived from streptozotocin-induced diabetic rats". International Journal of Molecular Medicine 31.3 (2013): 614-620.
Chicago
Zhao, Y., Zeng, D., Xia, L., Zhang, S., Xu, L., Jiang, X., Zhang, F."Osteogenic potential of bone marrow stromal cells derived from streptozotocin-induced diabetic rats". International Journal of Molecular Medicine 31, no. 3 (2013): 614-620. https://doi.org/10.3892/ijmm.2013.1227
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