Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
International Journal of Molecular Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1107-3756 Online ISSN: 1791-244X
Journal Cover
April 2013 Volume 31 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
April 2013 Volume 31 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Maintenance of high proliferation and multipotent potential of human hair follicle-derived mesenchymal stem cells by growth factors

  • Authors:
    • Xueyan Zhang
    • Yimei Wang
    • Yunhe Gao
    • Xuejuan Liu
    • Tingting Bai
    • Meiying  Li
    • Lisha Li
    • Guanfan Chi
    • Hui Xu
    • Feilin Liu
    • Jin Yu Liu
    • Yulin Li
  • View Affiliations / Copyright

    Affiliations: The Key Laboratory of Pathobiology, Ministry of Education, Jilin University, Changchun, Jilin 130021, P.R. China
  • Pages: 913-921
    |
    Published online on: February 6, 2013
       https://doi.org/10.3892/ijmm.2013.1272
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Cell therapy and cell-based tissue engineering is becoming increasingly important in regenerative medicine. Stem cells that are characterized by self-renewal, high proliferation and multiple differentiation potentials have attracted attention in cell-based regenerative medicine. Maintaining the aforementioned characteristics of stem cells is the first key step in cell-based regenerative medicine. Basic fibroblast growth factor (bFGF) is a well-known growth factor that efficiently maintains the self-renewal, high proliferation and multilineage differentiation potential of stem cells. Whether or not other growth factors, such as acidic fibroblast growth factor (aFGF) and epidermal growth factor (EGF) have similar effects has yet to be fully elucidated. Human hair follicle-derived mesenchymal stem cells (HF-MSCs) were obtained by organ culture. They exhibited surface markers of bone marrow mesenchymal stem cells as shown by positive staining for CD44, CD73, CD90 and CD105, and they also displayed trilineage differentiation potentials into adipocytes, chondrocytes and osteoblasts by cytochemistry and qRT-PCR. Flow cytometry analysis showed that up to 70% of HF-MSCs cultured in the presence of aFGF, bFGF or EGF stayed at the G0/G1 phase. Proliferation analysis showed that both bFGF and EGF at as low as 1 ng/ml and aFGF at above 5 ng/ml levels significantly increased the proliferation of HF-MSCs by cell counting. Consistent with proliferation analysis, immunofluorescence staining showed that more than 95% of HF-MSCs cultured in the presence of aFGF, bFGF and EGF were positively stained for proliferating cell nuclear antigen. HF-MSCs cultured in the presence of aFGF, bFGF or EGF retained marked trilineage differentiation potentials. By contrast, HF-MSCs cultured in the absence of bFGF, aFGF and EGF lost multipotency.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Park IH, Zhao R, West JA, et al: Reprogramming of human somatic cells to pluripotency with defined factors. Nature. 451:141–146. 2008. View Article : Google Scholar : PubMed/NCBI

2 

Liu JY, Peng HF, Gopinath S, Tian J and Andreadis ST: Derivation of functional smooth muscle cells from multipotent human hair follicle mesenchymal stem cells. Tissue Eng Part A. 16:2553–2564. 2010. View Article : Google Scholar : PubMed/NCBI

3 

Hoogduijn MJ, Gorjup E and Genever PG: Comparative characterization of hair follicle dermal stem cells and bone marrow mesenchymal stem cells. Stem Cell Dev. 15:49–60. 2006. View Article : Google Scholar : PubMed/NCBI

4 

Jahoda CA, Whitehouse J, Reynolds AJ and Hole N: Hair follicle dermal cells differentiate into adipogenic and osteogenic lineages. Exp Dermatol. 12:849–859. 2003. View Article : Google Scholar : PubMed/NCBI

5 

Rufaut NW, Goldthorpe NT, Wildermoth JE and Wallace OA: Myogenic differentiation of dermal papilla cells from bovine skin. J Cell Physiol. 209:959–966. 2006. View Article : Google Scholar : PubMed/NCBI

6 

Jahoda CA, Reynolds AJ, Chaponnier C, Forester JC and Gabbiani G: Smooth muscle alpha-actin is a maker for hair follicle dermis in vivo and in vitro. J Cell Sci. 99:627–636. 1991.PubMed/NCBI

7 

Lin X, Buff EM, Perrimon N and Michelson AM: Heparan sulfate proteoglycans are essential for FGF receptor signaling during Drosophila embryonic development. Development. 126:3715–3723. 1999.PubMed/NCBI

8 

Böhlen P, Esch F, Baird A and Gospodarowicz D: Acidic fibroblast growth factor (FGF) from bovine brain: amino-terminal sequence and comparison with basic FGF. EMBO J. 4:1951–1956. 1985.PubMed/NCBI

9 

Gospodarowicz D, Massoglia S, Cheng J and Fujii DK: Effect of fibroblast growth factor and lipoproteins on the proliferation of endothelial cells derived from bovine adrenal cortex, brain cortex, and corpus luteum capillaries. J Cell Physiol. 127:121–136. 1986. View Article : Google Scholar

10 

Vallier L, Alexander M and Pedersen RA: Activin/Nodal and FGF pathways cooperate to maintain pluripotency of human embryonic stem cells. J Cell Sci. 118:4495–4509. 2005. View Article : Google Scholar : PubMed/NCBI

11 

Wang G, Zhang H, Zhao Y, Li J, Cai J, Wang P, Meng S, Feng J, Miao C, Ding M, Li D and Deng H: Noggin and bFGF cooperate to maintain the pluripotency of humanembryonic stem cells in the absence of feeder layers. Biochem Biophys Res Commun. 330:934–942. 2005. View Article : Google Scholar : PubMed/NCBI

12 

Butterwith SC, Peddie CD and Goddard C: Regulation of adipocyte precursor DNA synthesis by acidic and basic fibroblast growth factors: interaction with heparin and other growth factors. J Endocrinol. 137:369–374. 1993. View Article : Google Scholar : PubMed/NCBI

13 

Hauner H, Röhrig K and Petruschke T: Effects of epidermal growth factor (EGF), platelet-derived growth factor (PDGF) and fibroblast growth factor (FGF) on human adipocyte development and function. Eur J Clin Invest. 25:90–96. 1995. View Article : Google Scholar : PubMed/NCBI

14 

Tamama K, Fan VH, Griffith LG, Blair HC and Wells A: Epidermal growth factor as a candidate for ex vivo expansion of bone marrow-derived mesenchymal stem cells. Stem Cells. 24:686–695. 2006. View Article : Google Scholar : PubMed/NCBI

15 

Zaragosi LE, Ailhaud G and Dani C: Autocrine fibroblast growth factor 2 signaling is critical for self-renewal of human multipotent adipose-derived stem cells. Stem Cells. 24:2412–2419. 2006. View Article : Google Scholar : PubMed/NCBI

16 

Toma JG, Akhavan M, Fernandes KJ, Barnabé-Heider F, Sadikot A, Kaplan DR and Miller FD: Isolation of multipotent adult stem cells from the dermis of mammalian skin. Nat Cell Biol. 3:778–784. 2001. View Article : Google Scholar : PubMed/NCBI

17 

Fernandes KJ, McKenzie IA, Mill P, et al: A dermal niche for multipotent adult skin-derived precursor cells. Nat Cell Biol. 6:1082–1093. 2004. View Article : Google Scholar : PubMed/NCBI

18 

Toma JG, McKenzie IA, Bagli D and Miller FD: Isolation and characterization of multipotent skin-derived precursors from human skin. Stem Cells. 23:727–737. 2005. View Article : Google Scholar : PubMed/NCBI

19 

Chen FG, Zhang WJ, Bi D, Liu W, Wei X, Chen FF, Zhu L, Cui L and Cao Y: Clonal analysis of nestin(−) vimentin(+) multipotent fibroblasts isolated from human dermis. J Cell Sci. 120:2875–2883. 2007.

20 

Lorenz K, Sicker M, Schmelzer E, Rupf T, Salvetter J, Schulz-Siegmund M and Bader A: Multilineage differentiation potential of human dermal skin-derived fibroblasts. Exp Dermatol. 17:925–932. 2008. View Article : Google Scholar : PubMed/NCBI

21 

Cotsarelis G, Sun TT and Lavker RM: Label-retaining cells reside in the bulge area of pilosebaceous unit: implications for follicular stem cells, hair cycle, and skin carcinogenesis. Cell. 61:1329–1337. 1990. View Article : Google Scholar : PubMed/NCBI

22 

Morris RJ and Potten CS: Highly persistent label-retaining cells in the hair follicles of mice and their fate following induction of anagen. J Invest Dermatol. 112:470–475. 1999. View Article : Google Scholar : PubMed/NCBI

23 

Tumbar T, Guasch G, Greco V, Blanpain C, Lowry WE, Rendl M and Fuchs E: Defining the epithelial stem cell niche in skin. Science. 303:359–363. 2004. View Article : Google Scholar : PubMed/NCBI

24 

Morris RJ, Liu Y, Marles L, Yang Z, Trempus C, Li S, Lin JS, Sawicki JA and Cotsarelis G: Capturing and profiling adult hair follicle stem cells. Nat Biotechnol. 22:411–417. 2004. View Article : Google Scholar : PubMed/NCBI

25 

Adachi H, Kurachi H, Homma H, et al: Epidermal growth factor promotes adipogenesis of 3T3-L1 cell in vitro. Endocrinology. 135:1824–1830. 1994.PubMed/NCBI

26 

Tsutsumi S, Shimazu A, Miyazaki K, Pan H, Koike C, Yoshida E, Takagishi K and Kato Y: Retention of multilineage differentiation potential of mesenchymal cells during proliferation in response to FGF. Biochem Biophys Res Commun. 288:413–419. 2001. View Article : Google Scholar : PubMed/NCBI

27 

Solchaga LA, Penick K, Porter JD, Goldberg VM, Caplan AI and Welter JF: FGF-2 enhances the mitotic and chondrogenic potentials of human adult bone marrow-derived mesenchymal stem cells. J Cell Physiol. 203:398–409. 2005. View Article : Google Scholar : PubMed/NCBI

28 

Martin I, Muraglia A, Campanile G, Cancedda R and Quarto R: Fibroblast growth factor-2 supports ex vivo expansion and maintenance of osteogenic precursors from human bone marrow. Endocrinology. 138:4456–4462. 1997.PubMed/NCBI

29 

Stewart AA, Byron CR, Pondenis H and Stewart MC: Effect of fibroblast growth factor-2 on equine mesenchymal stem cell monolayer expansion and chondrogenesis. Am J Vet Res. 68:941–945. 2007. View Article : Google Scholar : PubMed/NCBI

30 

Itoh N and Ornitz DM: Evolution of the Fgf and Fgfr gene families. Trends Genet. 20:563–569. 2004. View Article : Google Scholar : PubMed/NCBI

31 

Itoh N and Ornitz DM: Functional evolutionary history of the mouse Fgf gene family. Dev Dyn. 237:18–27. 2008. View Article : Google Scholar : PubMed/NCBI

32 

Thisse B and Thisse C: Functions and regulations of fibroblast growth factor signaling during embryonic development. Dev Biol. 287:390–402. 2005. View Article : Google Scholar : PubMed/NCBI

33 

Mikels AJ and Nusse R: Wnts as ligands: processing, secretion and reception. Oncogene. 25:7461–7468. 2006. View Article : Google Scholar : PubMed/NCBI

34 

Santiago-Mora R, Casado-Díaz A, De Castro MD and Quesada-Gómez JM: Oleuropein enhances osteoblastogenesis and inhibits adipogenesis: the effect on differentiation in stem cells derived from bone marrow. Osteoporos Int. 22:675–684. 2011. View Article : Google Scholar : PubMed/NCBI

35 

Choi KH, Choi BH, Park SR, Kim BJ and Min BH: The chondrogenic differentiation of mesenchymal stem cells on an extracellular matrix scaffold derived from porcine chondrocytes. Biomaterials. 31:5355–5365. 2010. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zhang X, Wang Y, Gao Y, Liu X, Bai T, Li M, Li L, Chi G, Xu H, Liu F, Liu F, et al: Maintenance of high proliferation and multipotent potential of human hair follicle-derived mesenchymal stem cells by growth factors. Int J Mol Med 31: 913-921, 2013.
APA
Zhang, X., Wang, Y., Gao, Y., Liu, X., Bai, T., Li, M. ... Li, Y. (2013). Maintenance of high proliferation and multipotent potential of human hair follicle-derived mesenchymal stem cells by growth factors. International Journal of Molecular Medicine, 31, 913-921. https://doi.org/10.3892/ijmm.2013.1272
MLA
Zhang, X., Wang, Y., Gao, Y., Liu, X., Bai, T., Li, M., Li, L., Chi, G., Xu, H., Liu, F., Liu, J. Y., Li, Y."Maintenance of high proliferation and multipotent potential of human hair follicle-derived mesenchymal stem cells by growth factors". International Journal of Molecular Medicine 31.4 (2013): 913-921.
Chicago
Zhang, X., Wang, Y., Gao, Y., Liu, X., Bai, T., Li, M., Li, L., Chi, G., Xu, H., Liu, F., Liu, J. Y., Li, Y."Maintenance of high proliferation and multipotent potential of human hair follicle-derived mesenchymal stem cells by growth factors". International Journal of Molecular Medicine 31, no. 4 (2013): 913-921. https://doi.org/10.3892/ijmm.2013.1272
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang X, Wang Y, Gao Y, Liu X, Bai T, Li M, Li L, Chi G, Xu H, Liu F, Liu F, et al: Maintenance of high proliferation and multipotent potential of human hair follicle-derived mesenchymal stem cells by growth factors. Int J Mol Med 31: 913-921, 2013.
APA
Zhang, X., Wang, Y., Gao, Y., Liu, X., Bai, T., Li, M. ... Li, Y. (2013). Maintenance of high proliferation and multipotent potential of human hair follicle-derived mesenchymal stem cells by growth factors. International Journal of Molecular Medicine, 31, 913-921. https://doi.org/10.3892/ijmm.2013.1272
MLA
Zhang, X., Wang, Y., Gao, Y., Liu, X., Bai, T., Li, M., Li, L., Chi, G., Xu, H., Liu, F., Liu, J. Y., Li, Y."Maintenance of high proliferation and multipotent potential of human hair follicle-derived mesenchymal stem cells by growth factors". International Journal of Molecular Medicine 31.4 (2013): 913-921.
Chicago
Zhang, X., Wang, Y., Gao, Y., Liu, X., Bai, T., Li, M., Li, L., Chi, G., Xu, H., Liu, F., Liu, J. Y., Li, Y."Maintenance of high proliferation and multipotent potential of human hair follicle-derived mesenchymal stem cells by growth factors". International Journal of Molecular Medicine 31, no. 4 (2013): 913-921. https://doi.org/10.3892/ijmm.2013.1272
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team