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International Journal of Molecular Medicine
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Article

Abnormal activation of complement C3 in the spinal dorsal horn is closely associated with progression of neuropathic pain

  • Authors:
    • Fachuan Nie
    • Jinbao Wang
    • Dong Su
    • Ying Shi
    • Jinmei Chen
    • Haihui Wang
    • Wanxiang Qin
    • Lin Shi
  • View Affiliations / Copyright

    Affiliations: Department of Pain Care and Nonvascular Intervention, The First Subordinate Hospital, Third Military Medical University, Chongqing, P.R. China, Department of Anesthesiology, Bethune Peace Hospital, Shijiazhuang, Hebei, P.R. China, Department of Anesthesiology, The First Subordinate Hospital, Third Military Medical University, Chongqing, P.R. China, Department of Anesthesiology, University Hospital of Brussels (UZ Brussel), Brussels, Belgium
  • Pages: 1333-1342
    |
    Published online on: April 11, 2013
       https://doi.org/10.3892/ijmm.2013.1344
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Abstract

The aim of the present study was to investigate the role of complement activation in the pathogenesis of neuropathic pain (NPP) induced by peripheral nerve injury. We modified a classical chronic constriction injury (CCI) model (mCCI), and verified its reliability in rats. Furthermore, reverse transcription-PCR and immunohistochemistry were conducted to investigate complement activation in the spinal dorsal horn and the effect of a complement inhibitor, cobra venom factor (CVF), on the behavior of the mCCI model rats. We found that rats in the mCCI group presented a better general condition, without signs of autophagy of the toes. Moreover, mCCI induced a significant increase (+40%) in the expression of component 3 (C3) mRNA in the spinal dorsal horn, which was associated with hyperalgesia. Correlation analysis showed a negative correlation between the mechanical pain threshold and the expression of C3 in the spinal cord. Administration of CVF reduced the occurrence of hyperalgesia in mCCI rats and nearly reversed the hyperalgesia. In addition, the mCCI rats exhibited significantly less spinal superoxide dismutase activity and significantly greater levels of maleic dialdehyde compared to the sham-operated rats. Transmission electron micrographs revealed mitochondrial swelling, cell membrane damage, and cristae fragmentation in the neurons of the spinal dorsal horn 14 days after mCCI. Mitochondrial swelling was attenuated in mCCI rats receiving CVF. The findings demonstrated that abnormal complement activation occurred in the dorsal horn of the spinal cord in rats with NPP, and C3 in the spinal dorsal horn could play an important role in the cascade reaction of complements that are involved in the development of hyperalgesia.
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Copy and paste a formatted citation
Spandidos Publications style
Nie F, Wang J, Su D, Shi Y, Chen J, Wang H, Qin W and Shi L: Abnormal activation of complement C3 in the spinal dorsal horn is closely associated with progression of neuropathic pain. Int J Mol Med 31: 1333-1342, 2013.
APA
Nie, F., Wang, J., Su, D., Shi, Y., Chen, J., Wang, H. ... Shi, L. (2013). Abnormal activation of complement C3 in the spinal dorsal horn is closely associated with progression of neuropathic pain. International Journal of Molecular Medicine, 31, 1333-1342. https://doi.org/10.3892/ijmm.2013.1344
MLA
Nie, F., Wang, J., Su, D., Shi, Y., Chen, J., Wang, H., Qin, W., Shi, L."Abnormal activation of complement C3 in the spinal dorsal horn is closely associated with progression of neuropathic pain". International Journal of Molecular Medicine 31.6 (2013): 1333-1342.
Chicago
Nie, F., Wang, J., Su, D., Shi, Y., Chen, J., Wang, H., Qin, W., Shi, L."Abnormal activation of complement C3 in the spinal dorsal horn is closely associated with progression of neuropathic pain". International Journal of Molecular Medicine 31, no. 6 (2013): 1333-1342. https://doi.org/10.3892/ijmm.2013.1344
Copy and paste a formatted citation
x
Spandidos Publications style
Nie F, Wang J, Su D, Shi Y, Chen J, Wang H, Qin W and Shi L: Abnormal activation of complement C3 in the spinal dorsal horn is closely associated with progression of neuropathic pain. Int J Mol Med 31: 1333-1342, 2013.
APA
Nie, F., Wang, J., Su, D., Shi, Y., Chen, J., Wang, H. ... Shi, L. (2013). Abnormal activation of complement C3 in the spinal dorsal horn is closely associated with progression of neuropathic pain. International Journal of Molecular Medicine, 31, 1333-1342. https://doi.org/10.3892/ijmm.2013.1344
MLA
Nie, F., Wang, J., Su, D., Shi, Y., Chen, J., Wang, H., Qin, W., Shi, L."Abnormal activation of complement C3 in the spinal dorsal horn is closely associated with progression of neuropathic pain". International Journal of Molecular Medicine 31.6 (2013): 1333-1342.
Chicago
Nie, F., Wang, J., Su, D., Shi, Y., Chen, J., Wang, H., Qin, W., Shi, L."Abnormal activation of complement C3 in the spinal dorsal horn is closely associated with progression of neuropathic pain". International Journal of Molecular Medicine 31, no. 6 (2013): 1333-1342. https://doi.org/10.3892/ijmm.2013.1344
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