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International Journal of Molecular Medicine
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Print ISSN: 1107-3756 Online ISSN: 1791-244X
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July 2013 Volume 32 Issue 1

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Article

Rutin, a bioflavonoid antioxidant protects rat pheochromocytoma (PC-12) cells against 6-hydroxydopamine (6-OHDA)-induced neurotoxicity

  • Authors:
    • Kasthuri Bai Magalingam
    • Ammu Radhakrishnan
    • Nagaraja Haleagrahara
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, Faculty of Medicine, International Medical University, Kuala Lumpur 57000, Malaysia, Discipline of Physiology and Pharmacology, School of Veterinary and Biomedical Sciences, James Cook University, Townsville, Queensland 4811, Australia
  • Pages: 235-240
    |
    Published online on: May 10, 2013
       https://doi.org/10.3892/ijmm.2013.1375
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Abstract

Free radicals are widely known to be the major cause of human diseases such as neurodegenerative diseases, cancer, allergy and autoimmune diseases. Human cells are equipped with a powerful natural antioxidant enzyme network. However, antioxidants, particularly those originating from natural sources such as fruits and vegetables, are still considered essential. Rutin, a quercetin glycoside, has been proven to possess antioxidant potential. However, the neuroprotective effect of rutin in pheochromocytoma (PC-12) cells has not been studied extensively. Therefore, the present study was designed to establish the neuroprotective role of rutin as well as to elucidate the antioxidant mechanism of rutin in 6-hydroxydopamine (6-OHDA)-induced toxicity in PC-12 neuronal cells. PC-12 cells were pretreated with different concentrations of rutin for 4, 8 and 12 h and subsequently incubated with 6-OHDA for 24 h to induce oxidative stress. A significant cytoprotective activity was observed in rutin pretreated cells in a dose-dependent manner. Furthermore, there was marked activation of antioxidant enzymes including superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx), and total glutathione (GSH) in rutin pretreated cells compared to cells incubated with 6-OHDA alone. Rutin significantly reduced lipid peroxidation in 6-OHDA-induced PC-12 cells. On the basis of these observations, it was concluded that the bioflavonoid rutin inhibited 6-OHDA-induced neurotoxicity in PC-12 cells by improving antioxidant enzyme levels and inhibiting lipid peroxidation.
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Copy and paste a formatted citation
Spandidos Publications style
Magalingam KB, Radhakrishnan A and Haleagrahara N: Rutin, a bioflavonoid antioxidant protects rat pheochromocytoma (PC-12) cells against 6-hydroxydopamine (6-OHDA)-induced neurotoxicity. Int J Mol Med 32: 235-240, 2013.
APA
Magalingam, K.B., Radhakrishnan, A., & Haleagrahara, N. (2013). Rutin, a bioflavonoid antioxidant protects rat pheochromocytoma (PC-12) cells against 6-hydroxydopamine (6-OHDA)-induced neurotoxicity. International Journal of Molecular Medicine, 32, 235-240. https://doi.org/10.3892/ijmm.2013.1375
MLA
Magalingam, K. B., Radhakrishnan, A., Haleagrahara, N."Rutin, a bioflavonoid antioxidant protects rat pheochromocytoma (PC-12) cells against 6-hydroxydopamine (6-OHDA)-induced neurotoxicity". International Journal of Molecular Medicine 32.1 (2013): 235-240.
Chicago
Magalingam, K. B., Radhakrishnan, A., Haleagrahara, N."Rutin, a bioflavonoid antioxidant protects rat pheochromocytoma (PC-12) cells against 6-hydroxydopamine (6-OHDA)-induced neurotoxicity". International Journal of Molecular Medicine 32, no. 1 (2013): 235-240. https://doi.org/10.3892/ijmm.2013.1375
Copy and paste a formatted citation
x
Spandidos Publications style
Magalingam KB, Radhakrishnan A and Haleagrahara N: Rutin, a bioflavonoid antioxidant protects rat pheochromocytoma (PC-12) cells against 6-hydroxydopamine (6-OHDA)-induced neurotoxicity. Int J Mol Med 32: 235-240, 2013.
APA
Magalingam, K.B., Radhakrishnan, A., & Haleagrahara, N. (2013). Rutin, a bioflavonoid antioxidant protects rat pheochromocytoma (PC-12) cells against 6-hydroxydopamine (6-OHDA)-induced neurotoxicity. International Journal of Molecular Medicine, 32, 235-240. https://doi.org/10.3892/ijmm.2013.1375
MLA
Magalingam, K. B., Radhakrishnan, A., Haleagrahara, N."Rutin, a bioflavonoid antioxidant protects rat pheochromocytoma (PC-12) cells against 6-hydroxydopamine (6-OHDA)-induced neurotoxicity". International Journal of Molecular Medicine 32.1 (2013): 235-240.
Chicago
Magalingam, K. B., Radhakrishnan, A., Haleagrahara, N."Rutin, a bioflavonoid antioxidant protects rat pheochromocytoma (PC-12) cells against 6-hydroxydopamine (6-OHDA)-induced neurotoxicity". International Journal of Molecular Medicine 32, no. 1 (2013): 235-240. https://doi.org/10.3892/ijmm.2013.1375
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