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International Journal of Molecular Medicine
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July 2013 Volume 32 Issue 1

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Article

miR-129-2 suppresses proliferation and migration of esophageal carcinoma cells through downregulation of SOX4 expression

  • Authors:
    • Min Kang
    • Yumei Li
    • Wenqi Liu
    • Rensheng Wang
    • Anzhou Tang
    • Hong Hao
    • Zhenguo Liu
    • Hesheng Ou
  • View Affiliations / Copyright

    Affiliations: The First Affiliated Hospital, Guangxi Medical University, Nanning, Guangxi, P.R. China, College of Pharmacy, Guangxi Medical University, Nanning, Guangxi, P.R. China, Davis Heart and Lung Research Institute and Division of Cardiovascular Medicine, The Ohio State University Medical Center, Columbus, OH, USA
  • Pages: 51-58
    |
    Published online on: May 16, 2013
       https://doi.org/10.3892/ijmm.2013.1384
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Abstract

We report the emerging role of microRNA (miRNA) deregulation associated with activation of an oncogene SOX4 (a member of the SRY-related HMG-box) in esophageal carcinoma. Paired esophageal cancer and adjacent non-tumor tissues were obtained from 42 patients who underwent primary surgical resection for esophageal cancer. Experiments such as real-time PCR, western blot analysis, luciferase-reporter assay, cell proliferation and colony formation assays, in vitro migration and invasion assays, and a wound-healing assay were performed to determine the effects of miR-129-2. We found that SOX4 expression was elevated (P<0.005) in esophageal tumors (n=42) when compared with its expression in the controls (n=42). Compared with the normal esophageal tissues, the expression of miR-129-2 was downregulated in 27 of 31 primary esophageal tumors, while the expression of SOX4 was upregulated (P<0.001). Restoration of miR-129-2 by transfection with an miRNA expression plasmid led to a decrease in SOX4 expression, which was accompanied by reduced migration and proliferation of the cancer cells. These results suggest that aberrant expression of SOX4 is associated with repression of miR-129-2, and restoration of miR-129-2 suppresses the migration and proliferation of esophageal cancer cells. Our results demonstrated that the deregulation of miR-129-2 leads to aberrant SOX4 expression, presenting a new paradigm in which the restoration of miRNA suppresses its oncogenic target in esophageal cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Kang M, Li Y, Liu W, Wang R, Tang A, Hao H, Liu Z and Ou H: miR-129-2 suppresses proliferation and migration of esophageal carcinoma cells through downregulation of SOX4 expression. Int J Mol Med 32: 51-58, 2013.
APA
Kang, M., Li, Y., Liu, W., Wang, R., Tang, A., Hao, H. ... Ou, H. (2013). miR-129-2 suppresses proliferation and migration of esophageal carcinoma cells through downregulation of SOX4 expression. International Journal of Molecular Medicine, 32, 51-58. https://doi.org/10.3892/ijmm.2013.1384
MLA
Kang, M., Li, Y., Liu, W., Wang, R., Tang, A., Hao, H., Liu, Z., Ou, H."miR-129-2 suppresses proliferation and migration of esophageal carcinoma cells through downregulation of SOX4 expression". International Journal of Molecular Medicine 32.1 (2013): 51-58.
Chicago
Kang, M., Li, Y., Liu, W., Wang, R., Tang, A., Hao, H., Liu, Z., Ou, H."miR-129-2 suppresses proliferation and migration of esophageal carcinoma cells through downregulation of SOX4 expression". International Journal of Molecular Medicine 32, no. 1 (2013): 51-58. https://doi.org/10.3892/ijmm.2013.1384
Copy and paste a formatted citation
x
Spandidos Publications style
Kang M, Li Y, Liu W, Wang R, Tang A, Hao H, Liu Z and Ou H: miR-129-2 suppresses proliferation and migration of esophageal carcinoma cells through downregulation of SOX4 expression. Int J Mol Med 32: 51-58, 2013.
APA
Kang, M., Li, Y., Liu, W., Wang, R., Tang, A., Hao, H. ... Ou, H. (2013). miR-129-2 suppresses proliferation and migration of esophageal carcinoma cells through downregulation of SOX4 expression. International Journal of Molecular Medicine, 32, 51-58. https://doi.org/10.3892/ijmm.2013.1384
MLA
Kang, M., Li, Y., Liu, W., Wang, R., Tang, A., Hao, H., Liu, Z., Ou, H."miR-129-2 suppresses proliferation and migration of esophageal carcinoma cells through downregulation of SOX4 expression". International Journal of Molecular Medicine 32.1 (2013): 51-58.
Chicago
Kang, M., Li, Y., Liu, W., Wang, R., Tang, A., Hao, H., Liu, Z., Ou, H."miR-129-2 suppresses proliferation and migration of esophageal carcinoma cells through downregulation of SOX4 expression". International Journal of Molecular Medicine 32, no. 1 (2013): 51-58. https://doi.org/10.3892/ijmm.2013.1384
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