Molecular basis for the expression of major vault protein induced by hyperosmotic stress in SW620 human colon cancer cells

Tajitsu,Yusuke; Ikeda,Ryuji; Nishizawa,Yukihiko; Mataki,Hirofumi; Che,Xiao-Fang; Sumizawa,Tomoyuki; Nitta,Mina; Yamaguchi,Tatsuya; Yamamoto,Masatatsu; Tabata,Sho; Akiyama,Shin-Ichi; Yamada,Katsushi; Furukawa,Tatsuhiko; Takeda,Yasuo

doi:10.3892/ijmm.2013.1428

Molecular basis for the expression of major vault protein induced by hyperosmotic stress in SW620 human colon cancer cells

Authors:
- Yusuke Tajitsu
- Ryuji Ikeda
- Yukihiko Nishizawa
- Hirofumi Mataki
- Xiao-Fang Che
- Tomoyuki Sumizawa
- Mina Nitta
- Tatsuya Yamaguchi
- Masatatsu Yamamoto
- Sho Tabata
- Shin-Ichi Akiyama
- Katsushi Yamada
- Tatsuhiko Furukawa
- Yasuo Takeda
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Affiliations: Department of Clinical Pharmacy and Pharmacology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8520, Japan, Department of Molecular Oncology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8520, Japan, Department of Domestic Science, Kagoshima Women's College, Kagoshima 890-8565, Japan, Department of Respiratory Medicine and Rheumatology, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima 770-8503, Japan, Department of Clinical Pharmacology, Faculty of Pharmaceutical Sciences, Nagasaki International University, Nagasaki 859-3298, Japan
Published online on: July 2, 2013 https://doi.org/10.3892/ijmm.2013.1428
Pages: 703-708

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Abstract

Major vault protein (MVP) is identical to lung resistance-related protein (LRP), which is the major component of vaults. Vaults are considered to play a protective role against xenobiotics and other types of stress. In a previous study, we reported that the expression levels of MVP in SW620 human colon cancer cells were increased in hypertonic culture medium with sucrose. However, the molecular mechanism behind the induction of MVP expression by osmotic stress has not yet been elucidated. Therefore, in the present study, we investigated the mechanism behind the induction of MVP expression by osmotic stress. Under hyperosmotic stress conditions, the ubiquitination of specificity protein 1 (Sp1) decreased, Sp1 protein levels increased, its binding to the MVP promoter was enhanced, and small interfering RNA (siRNA) for Sp1 suppressed the induction of MVP expression. The inhibition of c-jun N-terminal kinase (JNK) by SP600125, a specific JNK inhibitor, decreased the expression of MVP and Sp1 under hyperosmotic conditions. Our data indicate that the stabilization and upregulation of Sp1 protein expression by JNK participate in the inhibition of the ubiquitination and degradation of Sp1, and thus in the induction of MVP expression under hyperosmotic conditions.

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