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Article

Molecular basis for the expression of major vault protein induced by hyperosmotic stress in SW620 human colon cancer cells

  • Authors:
    • Yusuke Tajitsu
    • Ryuji Ikeda
    • Yukihiko Nishizawa
    • Hirofumi Mataki
    • Xiao-Fang Che
    • Tomoyuki Sumizawa
    • Mina Nitta
    • Tatsuya Yamaguchi
    • Masatatsu Yamamoto
    • Sho Tabata
    • Shin-Ichi Akiyama
    • Katsushi Yamada
    • Tatsuhiko Furukawa
    • Yasuo Takeda
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Pharmacy and Pharmacology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8520, Japan, Department of Molecular Oncology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8520, Japan, Department of Domestic Science, Kagoshima Women's College, Kagoshima 890-8565, Japan, Department of Respiratory Medicine and Rheumatology, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima 770-8503, Japan, Department of Clinical Pharmacology, Faculty of Pharmaceutical Sciences, Nagasaki International University, Nagasaki 859-3298, Japan
  • Pages: 703-708
    |
    Published online on: July 2, 2013
       https://doi.org/10.3892/ijmm.2013.1428
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Abstract

Major vault protein (MVP) is identical to lung resistance-related protein (LRP), which is the major component of vaults. Vaults are considered to play a protective role against xenobiotics and other types of stress. In a previous study, we reported that the expression levels of MVP in SW620 human colon cancer cells were increased in hypertonic culture medium with sucrose. However, the molecular mechanism behind the induction of MVP expression by osmotic stress has not yet been elucidated. Therefore, in the present study, we investigated the mechanism behind the induction of MVP expression by osmotic stress. Under hyperosmotic stress conditions, the ubiquitination of specificity protein 1 (Sp1) decreased, Sp1 protein levels increased, its binding to the MVP promoter was enhanced, and small interfering RNA (siRNA) for Sp1 suppressed the induction of MVP expression. The inhibition of c-jun N-terminal kinase (JNK) by SP600125, a specific JNK inhibitor, decreased the expression of MVP and Sp1 under hyperosmotic conditions. Our data indicate that the stabilization and upregulation of Sp1 protein expression by JNK participate in the inhibition of the ubiquitination and degradation of Sp1, and thus in the induction of MVP expression under hyperosmotic conditions.
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Copy and paste a formatted citation
Spandidos Publications style
Tajitsu Y, Ikeda R, Nishizawa Y, Mataki H, Che X, Sumizawa T, Nitta M, Yamaguchi T, Yamamoto M, Tabata S, Tabata S, et al: Molecular basis for the expression of major vault protein induced by hyperosmotic stress in SW620 human colon cancer cells. Int J Mol Med 32: 703-708, 2013.
APA
Tajitsu, Y., Ikeda, R., Nishizawa, Y., Mataki, H., Che, X., Sumizawa, T. ... Takeda, Y. (2013). Molecular basis for the expression of major vault protein induced by hyperosmotic stress in SW620 human colon cancer cells. International Journal of Molecular Medicine, 32, 703-708. https://doi.org/10.3892/ijmm.2013.1428
MLA
Tajitsu, Y., Ikeda, R., Nishizawa, Y., Mataki, H., Che, X., Sumizawa, T., Nitta, M., Yamaguchi, T., Yamamoto, M., Tabata, S., Akiyama, S., Yamada, K., Furukawa, T., Takeda, Y."Molecular basis for the expression of major vault protein induced by hyperosmotic stress in SW620 human colon cancer cells". International Journal of Molecular Medicine 32.3 (2013): 703-708.
Chicago
Tajitsu, Y., Ikeda, R., Nishizawa, Y., Mataki, H., Che, X., Sumizawa, T., Nitta, M., Yamaguchi, T., Yamamoto, M., Tabata, S., Akiyama, S., Yamada, K., Furukawa, T., Takeda, Y."Molecular basis for the expression of major vault protein induced by hyperosmotic stress in SW620 human colon cancer cells". International Journal of Molecular Medicine 32, no. 3 (2013): 703-708. https://doi.org/10.3892/ijmm.2013.1428
Copy and paste a formatted citation
x
Spandidos Publications style
Tajitsu Y, Ikeda R, Nishizawa Y, Mataki H, Che X, Sumizawa T, Nitta M, Yamaguchi T, Yamamoto M, Tabata S, Tabata S, et al: Molecular basis for the expression of major vault protein induced by hyperosmotic stress in SW620 human colon cancer cells. Int J Mol Med 32: 703-708, 2013.
APA
Tajitsu, Y., Ikeda, R., Nishizawa, Y., Mataki, H., Che, X., Sumizawa, T. ... Takeda, Y. (2013). Molecular basis for the expression of major vault protein induced by hyperosmotic stress in SW620 human colon cancer cells. International Journal of Molecular Medicine, 32, 703-708. https://doi.org/10.3892/ijmm.2013.1428
MLA
Tajitsu, Y., Ikeda, R., Nishizawa, Y., Mataki, H., Che, X., Sumizawa, T., Nitta, M., Yamaguchi, T., Yamamoto, M., Tabata, S., Akiyama, S., Yamada, K., Furukawa, T., Takeda, Y."Molecular basis for the expression of major vault protein induced by hyperosmotic stress in SW620 human colon cancer cells". International Journal of Molecular Medicine 32.3 (2013): 703-708.
Chicago
Tajitsu, Y., Ikeda, R., Nishizawa, Y., Mataki, H., Che, X., Sumizawa, T., Nitta, M., Yamaguchi, T., Yamamoto, M., Tabata, S., Akiyama, S., Yamada, K., Furukawa, T., Takeda, Y."Molecular basis for the expression of major vault protein induced by hyperosmotic stress in SW620 human colon cancer cells". International Journal of Molecular Medicine 32, no. 3 (2013): 703-708. https://doi.org/10.3892/ijmm.2013.1428
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