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Article

Inhibitory effects of STAT3 decoy oligodeoxynucleotides on human epithelial ovarian cancer cell growth in vivo

  • Authors:
    • Xiaolei Zhang
    • Peishu Liu
    • Bo Zhang
    • Hongluan Mao
    • Liang Shen
    • Yanhui Ma
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China, Department of Clinical Medicine, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China
  • Pages: 623-628
    |
    Published online on: July 4, 2013
       https://doi.org/10.3892/ijmm.2013.1431
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Abstract

The signal transducer and activator of transcription 3 (STAT3) regulates target gene expression by binding to a consensus DNA sequence within the promoter of the target genes. The constitutive activation of STAT3 has been shown to contribute to tumorigenesis in ovarian cancer and it has been reported to be a key factor for drug resistance in ovarian cancer. STAT3-specific decoy oligodeoxynucleotides (ODNs) (STAT3 decoy ODNs) that contain a consensus DNA sequence inhibit the transcriptional activity of STAT3, leading to cancer cell death. However, their mechanisms of action are unclear and little information is available as to the effects and the toxicity of STAT3 decoy ODNs in vivo. In this study, we established subcutaneous xenografts of SKOV3 human ovarian cancer cells in nude mice, evaluated the antitumor effects of STAT3 decoy ODNs on xenografted nude mice, and investigated the mechanisms behind the antitumor effects of STAT3 decoy ODNs targeting the STAT3 signaling pathway in vivo. The results revealed that the STAT3 decoy ODN inhibited ovarian cancer cell growth and promoted ovarian cancer cell apoptosis in vivo. Western blot analysis indicated that the STAT3 decoy ODN downregulated the protein expression levels of matrix metalloproteinase (MMP)-2, MMP-9 and Bcl-2, and upregulated the protein expression levels of caspase-3 in vivo. H&E staining was used to detect the side-effects of the STAT3 decoy ODN in the vital organs of the nude mice. We found that there were no significant abnormalities in the vital organs of the nude mice apart from slight inflammation and necrosis in parts of the hepatic lobule. The data from the present study suggest that decoy ODNs targeting STAT3 may be an effective therapeutic approach for the treatment of ovarian cancer in vivo.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang X, Liu P, Zhang B, Mao H, Shen L and Ma Y: Inhibitory effects of STAT3 decoy oligodeoxynucleotides on human epithelial ovarian cancer cell growth in vivo. Int J Mol Med 32: 623-628, 2013.
APA
Zhang, X., Liu, P., Zhang, B., Mao, H., Shen, L., & Ma, Y. (2013). Inhibitory effects of STAT3 decoy oligodeoxynucleotides on human epithelial ovarian cancer cell growth in vivo. International Journal of Molecular Medicine, 32, 623-628. https://doi.org/10.3892/ijmm.2013.1431
MLA
Zhang, X., Liu, P., Zhang, B., Mao, H., Shen, L., Ma, Y."Inhibitory effects of STAT3 decoy oligodeoxynucleotides on human epithelial ovarian cancer cell growth in vivo". International Journal of Molecular Medicine 32.3 (2013): 623-628.
Chicago
Zhang, X., Liu, P., Zhang, B., Mao, H., Shen, L., Ma, Y."Inhibitory effects of STAT3 decoy oligodeoxynucleotides on human epithelial ovarian cancer cell growth in vivo". International Journal of Molecular Medicine 32, no. 3 (2013): 623-628. https://doi.org/10.3892/ijmm.2013.1431
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang X, Liu P, Zhang B, Mao H, Shen L and Ma Y: Inhibitory effects of STAT3 decoy oligodeoxynucleotides on human epithelial ovarian cancer cell growth in vivo. Int J Mol Med 32: 623-628, 2013.
APA
Zhang, X., Liu, P., Zhang, B., Mao, H., Shen, L., & Ma, Y. (2013). Inhibitory effects of STAT3 decoy oligodeoxynucleotides on human epithelial ovarian cancer cell growth in vivo. International Journal of Molecular Medicine, 32, 623-628. https://doi.org/10.3892/ijmm.2013.1431
MLA
Zhang, X., Liu, P., Zhang, B., Mao, H., Shen, L., Ma, Y."Inhibitory effects of STAT3 decoy oligodeoxynucleotides on human epithelial ovarian cancer cell growth in vivo". International Journal of Molecular Medicine 32.3 (2013): 623-628.
Chicago
Zhang, X., Liu, P., Zhang, B., Mao, H., Shen, L., Ma, Y."Inhibitory effects of STAT3 decoy oligodeoxynucleotides on human epithelial ovarian cancer cell growth in vivo". International Journal of Molecular Medicine 32, no. 3 (2013): 623-628. https://doi.org/10.3892/ijmm.2013.1431
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