Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
International Journal of Molecular Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1107-3756 Online ISSN: 1791-244X
Journal Cover
September 2013 Volume 32 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
September 2013 Volume 32 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Polymorphisms and expression of the WNT8A gene in Hirschsprung's disease

  • Authors:
    • Hong Gao
    • Dong Chen
    • Xiaomei Liu
    • Mei Wu
    • Jie Mi
    • Weilin Wang
  • View Affiliations / Copyright

    Affiliations: Laboratory of Pediatric Congenital Malformation, Ministry of Public Health, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China
  • Pages: 647-652
    |
    Published online on: July 5, 2013
       https://doi.org/10.3892/ijmm.2013.1433
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Hirschsprung's disease (HSCR) is a congenital disorder characterized by an absence of intrinsic ganglion cells in the nerves forming the plexus of the lower intestine. The WNT signaling pathway is considered to play an important role in embryonic development. In the present study, we analyzed 2 polymorphisms of the WNT8A gene (rs78301778 and rs6596422) to determine their association with the risk and development of HSCR. Allele frequencies and genotype distributions were analyzed by sequence analysis in patients with HSCR and normal controls. Using real-time PCR, western blot analysis and immunohistochemistry, we detected the mRNA and protein expression of WNT8A in patients with HSCR. The data indicated that the differences in genotype distributions and allele frequencies of rs78301778 and rs6596422 between various clinical classifications were statistically significant. The analysis of the mRNA and protein expression of WNT8A revealed that the expression of WNT8A was increased in the stenotic colon segments compared with the normal colon segments. In conclusion, the data presented in this study suggest that the WNT8A gene is involved in the susceptibility to HSCR, and plays an important role in the occurrence and development of HSCR. These findings warrant further investigation.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Amiel J, Sproat-Emison E, Garcia-Barcelo M, Lantieri F, Burzynski G, Borrego S, Pelet A, Arnold S, Miao X, Griseri P, Brooks AS, Antinolo G, de Pontual L, Clement-Ziza M, Munnich A, Kashuk C, West K, Wong KK, Lyonnet S, Chakravarti A, Tam PK, Ceccherini I, Hofstra RM and Fernandez R: Hirschsprung disease, associated syndromes and genetics: a review. J Med Genet. 45:1–14. 2008. View Article : Google Scholar : PubMed/NCBI

2 

Grundy D and Schemann M: Enteric nervous system. Curr Opin Gastroenterol. 21:176–182. 2005. View Article : Google Scholar

3 

Lantieri F, Griseri P and Ceccherini I: Molecular mechanisms of RET-induced Hirschsprung pathogenesis. Ann Med. 38:11–19. 2006. View Article : Google Scholar : PubMed/NCBI

4 

Arighi E, Borrello MG and Sariola H: RET tyrosine kinase signaling in development and cancer. Cytokine Growth Factor Rev. 16:441–467. 2005. View Article : Google Scholar : PubMed/NCBI

5 

Tam PK and Garcia-Barcelo M: Molecular genetics of Hirschsprung's disease. Semin Pediatr Surg. 13:236–248. 2004. View Article : Google Scholar

6 

Sánchez-Mejías A, Fernández RM, López-Alonso M, Antiñolo G and Borrego S: New roles of EDNRB and EDN3 in the pathogenesis of Hirschsprung disease. Genet Med. 12:39–43. 2010.PubMed/NCBI

7 

Núñez-Torres R, Fernández RM, López-Alonso M, Antiñolo G and Borrego S: A novel study of copy number variations in Hirschsprung disease using the multiple ligation-dependent probe amplification (MLPA) technique. BMC Med Genet. 10:1192009.PubMed/NCBI

8 

Pan ZW, Lou J, Luo C, Yu L and Li JC: Association analysis of the SOX10 polymorphism with Hirschsprung disease in the Han Chinese population. J Pediatr Surg. 46:1930–1934. 2011. View Article : Google Scholar : PubMed/NCBI

9 

Fernandez RM, Ruiz-Ferrer M, Lopez-Alonso M, Antiñolo G and Borrego S: Polymorphisms in the genes encoding the 4 RET ligands, GDNF, NTN, ARTN, PSPN, and susceptibility to Hirschsprung disease. J Pediatr Surg. 43:2042–2047. 2008. View Article : Google Scholar : PubMed/NCBI

10 

Kwon MJ, Lee GH, Lee MK, Kim JY, Yoo HS, Ki CS, Chang YS, Kim JW and Park WS: PHOX2B mutations in patients with Ondine-Hirschsprung disease and a review of the literature. Eur J Pediatr. 170:1267–1271. 2011. View Article : Google Scholar : PubMed/NCBI

11 

Jiang Q, Ho YY, Hao L, Nichols Berrios C and Chakravarti A: Copy number variants in candidate genes are genetic modifiers of Hirschsprung disease. PLoS One. 6:e212192011. View Article : Google Scholar : PubMed/NCBI

12 

Gregory-Evans CY, Vieira H, Dalton R, Adams GG, Salt A and Gregory-Evans K: Ocular coloboma and high myopia with Hirschsprung disease associated with a novel ZFHX1B missense mutation and trisomy 21. Am J Med Genet A. 131:86–90. 2004. View Article : Google Scholar : PubMed/NCBI

13 

Bai Y, Wang Z, Dai W, Li Q, Chen G, Cong N, Guan M and Li H: A six-generation Chinese family in haplogroup B4C1C exhibits high penetrance of 1555A > G-induced hearing loss. BMC Med Genet. 11:1292010. View Article : Google Scholar : PubMed/NCBI

14 

Ramel MC and Lekven AC: Repression of the vertebrate organizer by Wnt8 is mediated by Vent and Vox. Development. 131:3991–4000. 2004. View Article : Google Scholar : PubMed/NCBI

15 

Martin BL and Kimelman D: Regulation of canonical Wnt signaling by Brachyury is essential for posterior mesoderm formation. Dev Cell. 15:121–133. 2008. View Article : Google Scholar : PubMed/NCBI

16 

Baker KD, Ramel MC and Lekven AC: A direct role for Wnt8 in ventrolateral mesoderm patterning. Dev Dyn. 239:2828–2836. 2010. View Article : Google Scholar : PubMed/NCBI

17 

Erter CE, Wilm TP, Basler N, Wright CV and Solnica-Krezel L: Wnt8 is required in lateral mesendodermal precursors for neural posteriorization in vivo. Development. 128:3571–3583. 2001.PubMed/NCBI

18 

Rhinn M, Lun K, Luz M, Werner M and Brand M: Positioning of the midbrain-hindbrain boundary organizer through global posteriorization of the neuroectoderm mediated by Wnt8 signaling. Development. 132:1261–1272. 2005. View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Gao H, Chen D, Liu X, Wu M, Mi J and Wang W: Polymorphisms and expression of the WNT8A gene in Hirschsprung's disease. Int J Mol Med 32: 647-652, 2013.
APA
Gao, H., Chen, D., Liu, X., Wu, M., Mi, J., & Wang, W. (2013). Polymorphisms and expression of the WNT8A gene in Hirschsprung's disease. International Journal of Molecular Medicine, 32, 647-652. https://doi.org/10.3892/ijmm.2013.1433
MLA
Gao, H., Chen, D., Liu, X., Wu, M., Mi, J., Wang, W."Polymorphisms and expression of the WNT8A gene in Hirschsprung's disease". International Journal of Molecular Medicine 32.3 (2013): 647-652.
Chicago
Gao, H., Chen, D., Liu, X., Wu, M., Mi, J., Wang, W."Polymorphisms and expression of the WNT8A gene in Hirschsprung's disease". International Journal of Molecular Medicine 32, no. 3 (2013): 647-652. https://doi.org/10.3892/ijmm.2013.1433
Copy and paste a formatted citation
x
Spandidos Publications style
Gao H, Chen D, Liu X, Wu M, Mi J and Wang W: Polymorphisms and expression of the WNT8A gene in Hirschsprung's disease. Int J Mol Med 32: 647-652, 2013.
APA
Gao, H., Chen, D., Liu, X., Wu, M., Mi, J., & Wang, W. (2013). Polymorphisms and expression of the WNT8A gene in Hirschsprung's disease. International Journal of Molecular Medicine, 32, 647-652. https://doi.org/10.3892/ijmm.2013.1433
MLA
Gao, H., Chen, D., Liu, X., Wu, M., Mi, J., Wang, W."Polymorphisms and expression of the WNT8A gene in Hirschsprung's disease". International Journal of Molecular Medicine 32.3 (2013): 647-652.
Chicago
Gao, H., Chen, D., Liu, X., Wu, M., Mi, J., Wang, W."Polymorphisms and expression of the WNT8A gene in Hirschsprung's disease". International Journal of Molecular Medicine 32, no. 3 (2013): 647-652. https://doi.org/10.3892/ijmm.2013.1433
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team