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Article

Treatment of liver cancer in mice by the intratumoral injection of an octreotide-based temperature‑sensitive gel

  • Authors:
    • Lili Zhang
    • Su Yu
    • Zhijun Duan
    • Qiuming Wang
    • Ge Tian
    • Yan Tian
    • Wei Zhao
    • Hui Wang
    • Cuiling Zhang
    • Shibin Guo
    • Qigui Liu
    • Gaohong He
    • Tengfei Bian
    • Jiuyang Chang
    • Xue Jin
    • Dongsheng Cui
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, The First Affiliated Hospital, Dalian Medical University, Dalian, Liaoning 116011, P.R. China, Department of Clinical Pharmacy, College of Pharmacy, Dalian Medical University, Dalian, Liaoning 116011, P.R. China, Morphology Laboratory, College of Basic of Medical Sciences, Dalian Medical University, Dalian, Liaoning 116011, P.R. China, Department of Ultrasound, The First Affiliated Hospital, Dalian Medical University, Dalian, Liaoning 116011, P.R. China, Department of Clinical Laboratory, The First Affiliated Hospital, Dalian Medical University, Dalian, Liaoning 116011, P.R. China, Department of Digestive Endoscopy, The First Affiliated Hospital, Dalian Medical University, Dalian, Liaoning 116011, P.R. China, Dalian Medical University School of Public Health Research Statistics, Dalian, Liaoning 116011, P.R. China, Institute of Chemical Engineering, Dalian University of Technology, Dalian, Liaoning 116024, P.R. China, Department of Anesthesiology, Dalian Medical University, Dalian, Liaoning 116011, P.R. China
  • Pages: 117-127
    |
    Published online on: October 30, 2013
       https://doi.org/10.3892/ijmm.2013.1542
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Abstract

Octreotide (OCT) can inhibit tumor growth with few side‑effects. In this study, we hypothesized that an OCT- and poloxamer 407 (P407)-based temperature‑sensitive gel may compensate for the short half‑life of OCT, which may thus lead to the development of a novel therapy for patients with end‑stage liver cancer by intratumoral injection. The proliferation and apoptosis of mouse Hca‑F hepatocellular carcinoma cells were determined by MTT assay and Annexin V‑PI staining. A mouse model of hepatocellular carcinoma was established by the subcutaneous transplantion of Hca‑F cells and OCT‑P407 or OCT solution were injected into the tumors, followed by the detection of OCT levels by high performance liquid chromatography (HPLC) over a specific time period. OCT‑P407, ethanol, OCT, P407 or normal saline (NS) were injected into the tumors and the tumor size, weight and inhibition rate were measured 8 days later. Additionally, the expression of somatostatin receptor‑2 (SSTR‑2), vascular endothelial growth factor (VEGF) and caspase‑3 was detected by immunohistochemistry and RT‑PCR. Compared with the OCT group, the tumor inhibition rate and the apoptotic rate in the OCT‑P407 group were higher and the effects were longer. The tumor size and weight in the OCT‑P407 group were lower and the tumor inhibition rate higher compared with the OCT, P407 and NS groups, with the exception of the ethanol group. The protein and mRNA expression of SSTR‑2 and caspase‑3 in the OCT‑P407 group was higher, and that of VEFG was lower compared with the other groups, with the exception of the ethanol group. In the present study, we demonstrate that the intratumoral injection of OCT‑P407 maintains OCT local effective concentration and prolongs its action time, with a greater therapeutic effect than that of OCT on its own. Although ethanol is more effective in certain aspects, its tumor inhibitory effects are similar to OCT‑P407 and as such, OCT‑P407 may be a suitable alternative.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang L, Yu S, Duan Z, Wang Q, Tian G, Tian Y, Zhao W, Wang H, Zhang C, Guo S, Guo S, et al: Treatment of liver cancer in mice by the intratumoral injection of an octreotide-based temperature‑sensitive gel. Int J Mol Med 33: 117-127, 2014.
APA
Zhang, L., Yu, S., Duan, Z., Wang, Q., Tian, G., Tian, Y. ... Cui, D. (2014). Treatment of liver cancer in mice by the intratumoral injection of an octreotide-based temperature‑sensitive gel. International Journal of Molecular Medicine, 33, 117-127. https://doi.org/10.3892/ijmm.2013.1542
MLA
Zhang, L., Yu, S., Duan, Z., Wang, Q., Tian, G., Tian, Y., Zhao, W., Wang, H., Zhang, C., Guo, S., Liu, Q., He, G., Bian, T., Chang, J., Jin, X., Cui, D."Treatment of liver cancer in mice by the intratumoral injection of an octreotide-based temperature‑sensitive gel". International Journal of Molecular Medicine 33.1 (2014): 117-127.
Chicago
Zhang, L., Yu, S., Duan, Z., Wang, Q., Tian, G., Tian, Y., Zhao, W., Wang, H., Zhang, C., Guo, S., Liu, Q., He, G., Bian, T., Chang, J., Jin, X., Cui, D."Treatment of liver cancer in mice by the intratumoral injection of an octreotide-based temperature‑sensitive gel". International Journal of Molecular Medicine 33, no. 1 (2014): 117-127. https://doi.org/10.3892/ijmm.2013.1542
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x
Spandidos Publications style
Zhang L, Yu S, Duan Z, Wang Q, Tian G, Tian Y, Zhao W, Wang H, Zhang C, Guo S, Guo S, et al: Treatment of liver cancer in mice by the intratumoral injection of an octreotide-based temperature‑sensitive gel. Int J Mol Med 33: 117-127, 2014.
APA
Zhang, L., Yu, S., Duan, Z., Wang, Q., Tian, G., Tian, Y. ... Cui, D. (2014). Treatment of liver cancer in mice by the intratumoral injection of an octreotide-based temperature‑sensitive gel. International Journal of Molecular Medicine, 33, 117-127. https://doi.org/10.3892/ijmm.2013.1542
MLA
Zhang, L., Yu, S., Duan, Z., Wang, Q., Tian, G., Tian, Y., Zhao, W., Wang, H., Zhang, C., Guo, S., Liu, Q., He, G., Bian, T., Chang, J., Jin, X., Cui, D."Treatment of liver cancer in mice by the intratumoral injection of an octreotide-based temperature‑sensitive gel". International Journal of Molecular Medicine 33.1 (2014): 117-127.
Chicago
Zhang, L., Yu, S., Duan, Z., Wang, Q., Tian, G., Tian, Y., Zhao, W., Wang, H., Zhang, C., Guo, S., Liu, Q., He, G., Bian, T., Chang, J., Jin, X., Cui, D."Treatment of liver cancer in mice by the intratumoral injection of an octreotide-based temperature‑sensitive gel". International Journal of Molecular Medicine 33, no. 1 (2014): 117-127. https://doi.org/10.3892/ijmm.2013.1542
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