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International Journal of Molecular Medicine
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2014-March Volume 33 Issue 3

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International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Article

Phytosphigosine-1-phosphate increases sensitivity of EGF-dependent cell proliferation

  • Authors:
    • Hwa Jun Cha
    • Jeong Pyo Lee
    • Kwang Sik Lee
    • Kun Kook Lee
    • Myoung Jun Choi
    • Dong Kyu Lee
    • Kwang Nyeon Kim
    • Sungkwan An
  • View Affiliations / Copyright

    Affiliations: Molecular-Targeted Drug Research Center and Korea Institute for Skin and Clinical Sciences, Konkuk University, Gwangjin-gu, Seoul 143-701, Republic of Korea, Coreana Cosmetics Co., Ltd., Seonggeo-eup, Seobuk-gu, Cheonan-si, Chungcheongnam-do 330-833, Republic of Korea, Phytos Co. Ltd., No. 609, Industry-Academic Collaboration Foundation, Yeongtong-gu, Gyeonggi-do 443-760, Republic of Korea, Industrial Engineering Chemistry, Chungbuk National University, Heungdeok-gu, Cheongju, Chungcheongbuk-do 361-763, Republic of Korea, Damy Chemical Co., Ltd., No. 308, Byuk-San Digital Valley II, Keumchun-gu, Seoul 153-803, Republic of Korea
  • Pages: 649-653
    |
    Published online on: January 7, 2014
       https://doi.org/10.3892/ijmm.2014.1617
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Abstract

The dermis is composed of dermal fibroblasts and various synthesized extracellular matrices. Proliferation of these cells is important to skin structure homeostasis. Therefore, human dermal fibroblasts (HDFs) growth factors have been previously evaluated. In the present study, we examined whether phytosphingosine‑1-phosphate (PhS1P) regulates gene expression, particularly cell cycle-related genes. In addition, we investigated whether there was a synergistic effect of proliferation induced by PhS1P and epidermal growth factor (EGF) through PhS1P-regulated genes. A microarray approach was utilized to identify gene expression changes in PhS1P-treated HDFs and data were analyzed using gene ontology (GO). In addition, proliferative synergistic effects were measured using an MTT assay. The results showed that PhS1P regulates various genes, particularly cell cycle-related genes. Microarray data, followed by GO, indicated that PhS1P affected the biological processes involved in the cell cycle (cyclins A2, B1 and B2). Furthermore, these genes synergistically affected EGF-dependent proliferation. The results obtained in this study demonstrated that PhS1P altered gene expression profiles, inducing EGF-dependent cell proliferation. Therefore, PhS1p acts as a synergistic effector for EGF.
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Copy and paste a formatted citation
Spandidos Publications style
Cha HJ, Lee JP, Lee KS, Lee KK, Choi MJ, Lee DK, Kim KN and An S: Phytosphigosine-1-phosphate increases sensitivity of EGF-dependent cell proliferation. Int J Mol Med 33: 649-653, 2014.
APA
Cha, H.J., Lee, J.P., Lee, K.S., Lee, K.K., Choi, M.J., Lee, D.K. ... An, S. (2014). Phytosphigosine-1-phosphate increases sensitivity of EGF-dependent cell proliferation. International Journal of Molecular Medicine, 33, 649-653. https://doi.org/10.3892/ijmm.2014.1617
MLA
Cha, H. J., Lee, J. P., Lee, K. S., Lee, K. K., Choi, M. J., Lee, D. K., Kim, K. N., An, S."Phytosphigosine-1-phosphate increases sensitivity of EGF-dependent cell proliferation". International Journal of Molecular Medicine 33.3 (2014): 649-653.
Chicago
Cha, H. J., Lee, J. P., Lee, K. S., Lee, K. K., Choi, M. J., Lee, D. K., Kim, K. N., An, S."Phytosphigosine-1-phosphate increases sensitivity of EGF-dependent cell proliferation". International Journal of Molecular Medicine 33, no. 3 (2014): 649-653. https://doi.org/10.3892/ijmm.2014.1617
Copy and paste a formatted citation
x
Spandidos Publications style
Cha HJ, Lee JP, Lee KS, Lee KK, Choi MJ, Lee DK, Kim KN and An S: Phytosphigosine-1-phosphate increases sensitivity of EGF-dependent cell proliferation. Int J Mol Med 33: 649-653, 2014.
APA
Cha, H.J., Lee, J.P., Lee, K.S., Lee, K.K., Choi, M.J., Lee, D.K. ... An, S. (2014). Phytosphigosine-1-phosphate increases sensitivity of EGF-dependent cell proliferation. International Journal of Molecular Medicine, 33, 649-653. https://doi.org/10.3892/ijmm.2014.1617
MLA
Cha, H. J., Lee, J. P., Lee, K. S., Lee, K. K., Choi, M. J., Lee, D. K., Kim, K. N., An, S."Phytosphigosine-1-phosphate increases sensitivity of EGF-dependent cell proliferation". International Journal of Molecular Medicine 33.3 (2014): 649-653.
Chicago
Cha, H. J., Lee, J. P., Lee, K. S., Lee, K. K., Choi, M. J., Lee, D. K., Kim, K. N., An, S."Phytosphigosine-1-phosphate increases sensitivity of EGF-dependent cell proliferation". International Journal of Molecular Medicine 33, no. 3 (2014): 649-653. https://doi.org/10.3892/ijmm.2014.1617
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