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International Journal of Molecular Medicine
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2014-April Volume 33 Issue 4

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Article

Developmental endothelial locus-1 inhibits MIF production through suppression of NF-κB in macrophages

  • Authors:
    • Seung-Hwan Lee
    • Dong-Young Kim
    • Yoon-Young Kang
    • Hyesoon Kim
    • Jungin Jang
    • Mi-Ni Lee
    • Goo Taeg Oh
    • Sang-Wook Kang
    • Eun Young Choi
  • View Affiliations / Copyright

    Affiliations: Department of Biomedical Sciences, University of Ulsan College of Medicine, Seoul, Republic of Korea, Division of Life Sciences, Ewha Womans University, Seoul, Republic of Korea
  • Pages: 919-924
    |
    Published online on: February 5, 2014
       https://doi.org/10.3892/ijmm.2014.1645
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Abstract

Macrophage migration inhibitory factor (MIF) is a proinflammatory cytokine that regulates leukocyte recruitment, thereby playing a pivotal role in the regulation of innate and adaptive immunity and tumor progression. Elevated levels of MIF are associated with numerous inflammatory disorders and cancers. To determine whether developmental endothelial locus-1 (Del-1) regulated MIF, RAW264.7 macrophages were treated with Del-1 and assessed using ELISA. The results showed that MIF was downregulated in macrophages by Del-1, an endogenous anti-inflammatory protein that was previously shown to limit leukocyte adhesion and migration. Treatment of RAW264.7 macrophages with Del-1 inhibited constitutive and lipopolysaccharide (LPS)-induced MIF secretion. Recombinant Del-1 protein attenuated the phosphorylation of IκBα induced by a relatively low concentration of LPS in THP-1 monocytes, but did not inhibit IκBα phosphorylation in response to a relatively high concentration of LPS. Concomitantly, translocation of NF-κB to the nucleus was inhibited by Del-1 in LPS-activated macrophages. In addition, conditioned medium harvested from cells transfected with a Del-1 expression plasmid suppressed NF-κB activation in response to relatively low concentrations of TNF-α, albeit not the activation that was induced by a relatively high concentration of TNF-α. On the other hand, although Del-1 enhanced the macrophage expression of p53, a known negative regulator of MIF production, MIF production was not significantly affected by the level of p53 in mouse bone marrow-derived macrophages. These findings suggested that Del-1 controls NF-κB-activated MIF production in macrophages, and the potential application of Del-1 to therapeutic modalities for chronic inflammation-associated cancers.
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View References

1 

Bloom BR and Bennett B: Mechanism of a reaction in vitro associated with delayed-type hypersensitivity. Science. 153:80–82. 1966. View Article : Google Scholar : PubMed/NCBI

2 

David JR: Delayed hypersensitivity in vitro: its mediation by cell-free substances formed by lymphoid cell-antigen interaction. Proc Natl Acad Sci USA. 56:72–77. 1966. View Article : Google Scholar : PubMed/NCBI

3 

Gregory JL, Morand EF, McKeown SJ, et al: Macrophage migration inhibitory factor induces macrophage recruitment via CC chemokine ligand 2. J Immunol. 177:8072–8079. 2006. View Article : Google Scholar : PubMed/NCBI

4 

Bernhagen J, Krohn R, Lue H, et al: MIF is a noncognate ligand of CXC chemokine receptors in inflammatory and atherogenic cell recruitment. Nat Med. 13:587–596. 2007. View Article : Google Scholar : PubMed/NCBI

5 

Yoshihisa Y, Makino T, Matsunaga K, et al: Macrophage migration inhibitory factor is essential for eosinophil recruitment in allergen-induced skin inflammation. J Invest Dermatol. 131:925–931. 2011. View Article : Google Scholar : PubMed/NCBI

6 

Li J, Mo HY, Xiong G, et al: Tumor microenvironment macrophage inhibitory factor directs the accumulation of interleukin-17-producing tumor-infiltrating lymphocytes and predicts favorable survival in nasopharyngeal carcinoma patients. J Biol Chem. 287:35484–35495. 2012. View Article : Google Scholar

7 

Calandra T and Roger T: Macrophage migration inhibitory factor: a regulator of innate immunity. Nat Rev Immunol. 3:791–800. 2003. View Article : Google Scholar : PubMed/NCBI

8 

Rossi AG, Haslett C, Hirani N, et al: Human circulating eosinophils secrete macrophage migration inhibitory factor (MIF). Potential role in asthma. J Clin Invest. 101:2869–2874. 1998. View Article : Google Scholar : PubMed/NCBI

9 

Veillat V, Lavoie CH, Metz CN, Roger T, Labelle Y and Akoum A: Involvement of nuclear factor-kappaB in macrophage migration inhibitory factor gene transcription up-regulation induced by interleukin-1 beta in ectopic endometrial cells. Fertil Steril. 91:2148–2156. 2009. View Article : Google Scholar : PubMed/NCBI

10 

Leng L, Metz CN, Fang Y, et al: MIF signal transduction initiated by binding to CD74. J Exp Med. 197:1467–1476. 2003. View Article : Google Scholar : PubMed/NCBI

11 

Amin MA, Haas CS, Zhu K, et al: Migration inhibitory factor up-regulates vascular cell adhesion molecule-1 and intercellular adhesion molecule-1 via Src, PI3 kinase, and NFkappaB. Blood. 107:2252–2261. 2006. View Article : Google Scholar : PubMed/NCBI

12 

Napetschnig J and Wu H: Molecular basis of NF-kappaB signaling. Annu Rev Biophys. 42:443–468. 2013. View Article : Google Scholar

13 

Mizue Y, Ghani S, Leng L, et al: Role for macrophage migration inhibitory factor in asthma. Proc Natl Acad Sci USA. 102:14410–14415. 2005. View Article : Google Scholar : PubMed/NCBI

14 

Calandra T, Echtenacher B, Roy DL, et al: Protection from septic shock by neutralization of macrophage migration inhibitory factor. Nat Med. 6:164–170. 2000. View Article : Google Scholar : PubMed/NCBI

15 

de Jong YP, Abadia-Molina AC, Satoskar AR, et al: Development of chronic colitis is dependent on the cytokine MIF. Nat Immunol. 2:1061–1066. 2001.PubMed/NCBI

16 

Baugh JA, Chitnis S, Donnelly SC, et al: A functional promoter polymorphism in the macrophage migration inhibitory factor (MIF) gene associated with disease severity in rheumatoid arthritis. Genes Immun. 3:170–176. 2002. View Article : Google Scholar : PubMed/NCBI

17 

Plant BJ, Gallagher CG, Bucala R, et al: Cystic fibrosis, disease severity, and a macrophage migration inhibitory factor polymorphism. Am J Respir Crit Care Med. 172:1412–1415. 2005. View Article : Google Scholar : PubMed/NCBI

18 

Miller EJ, Li J, Leng L, et al: Macrophage migration inhibitory factor stimulates AMP-activated protein kinase in the ischaemic heart. Nature. 451:578–582. 2008. View Article : Google Scholar : PubMed/NCBI

19 

Nohara H, Okayama N, Inoue N, et al: Association of the −173 G/C polymorphism of the macrophage migration inhibitory factor gene with ulcerative colitis. J Gastroenterol. 39:242–246. 2004.

20 

Mitchell RA, Liao H, Chesney J, et al: Macrophage migration inhibitory factor (MIF) sustains macrophage proinflammatory function by inhibiting p53: regulatory role in the innate immune response. Proc Natl Acad Sci USA. 99:345–350. 2002. View Article : Google Scholar : PubMed/NCBI

21 

Hudson JD, Shoaibi MA, Maestro R, Carnero A, Hannon GJ and Beach DH: A proinflammatory cytokine inhibits p53 tumor suppressor activity. J Exp Med. 190:1375–1382. 1999. View Article : Google Scholar : PubMed/NCBI

22 

Cooke G, Armstrong ME and Donnelly SC: Macrophage migration inhibitory factor (MIF), enzymatic activity and the inflammatory response. Biofactors. 35:165–168. 2009. View Article : Google Scholar : PubMed/NCBI

23 

Stosic-Grujicic S, Stojanovic I and Nicoletti F: MIF in autoimmunity and novel therapeutic approaches. Autoimmun Rev. 8:244–249. 2009. View Article : Google Scholar : PubMed/NCBI

24 

Xu L, Li Y, Sun H, et al: Current developments of macrophage migration inhibitory factor (MIF) inhibitors. Drug Discov Today. 18:592–600. 2013. View Article : Google Scholar : PubMed/NCBI

25 

Hanayama R, Tanaka M, Miwa K and Nagata S: Expression of developmental endothelial locus-1 in a subset of macrophages for engulfment of apoptotic cells. J Immunol. 172:3876–3882. 2004. View Article : Google Scholar : PubMed/NCBI

26 

Hidai C, Zupancic T, Penta K, et al: Cloning and characterization of developmental endothelial locus-1: an embryonic endothelial cell protein that binds the alphavbeta3 integrin receptor. Genes Dev. 12:21–33. 1998. View Article : Google Scholar : PubMed/NCBI

27 

Rezaee M, Penta K and Quertermous T: Del1 mediates VSMC adhesion, migration, and proliferation through interaction with integrin alpha(v)beta(3). Am J Physiol Heart Circ Physiol. 282:H1924–H1932. 2002.PubMed/NCBI

28 

Wang Z, Kundu RK, Longaker MT, Quertermous T and Yang GP: The angiogenic factor Del1 prevents apoptosis of endothelial cells through integrin binding. Surgery. 151:296–305. 2012. View Article : Google Scholar : PubMed/NCBI

29 

Choi EY, Chavakis E, Czabanka MA, et al: Del-1, an endogenous leukocyte-endothelial adhesion inhibitor, limits inflammatory cell recruitment. Science. 322:1101–1104. 2008. View Article : Google Scholar : PubMed/NCBI

30 

Choi EY, Orlova VV, Fagerholm SC, et al: Regulation of LFA-1-dependent inflammatory cell recruitment by Cbl-b and 14-3-3 proteins. Blood. 111:3607–3614. 2008. View Article : Google Scholar : PubMed/NCBI

31 

Kim H, Lee SH, Lee MN, Oh GT, Choi KC and Choi EY: p53 regulates the transcription of the anti-inflammatory molecule developmental endothelial locus-1 (Del-1). Oncotarget. 4:1976–1985. 2013.PubMed/NCBI

32 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods. 25:402–408. 2001.

33 

Salminen A and Kaarniranta K: Control of p53 and NF-kappaB signaling by WIP1 and MIF: role in cellular senescence and organismal aging. Cell Signal. 23:747–752. 2011. View Article : Google Scholar : PubMed/NCBI

34 

Webster GA and Perkins ND: Transcriptional cross talk between NF-kappaB and p53. Mol Cell Biol. 19:3485–3495. 1999.PubMed/NCBI

35 

Blagosklonny MV, An WG, Romanova LY, Trepel J, Fojo T and Neckers L: p53 inhibits hypoxia-inducible factor-stimulated transcription. J Biol Chem. 273:11995–11998. 1998. View Article : Google Scholar : PubMed/NCBI

36 

Blouin CC, Pagé EL, Soucy GM and Richard DE: Hypoxic gene activation by lipopolysaccharide in macrophages: implication of hypoxia-inducible factor 1alpha. Blood. 103:1124–1130. 2004. View Article : Google Scholar : PubMed/NCBI

37 

Ortiz-Barahona A, Villar D, Pescador N, Amigo J and del Peso L: Genome-wide identification of hypoxia-inducible factor binding sites and target genes by a probabilistic model integrating transcription-profiling data and in silico binding site prediction. Nucleic Acids Res. 38:2332–2345. 2010. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Lee S, Kim D, Kang Y, Kim H, Jang J, Lee M, Oh GT, Kang S and Choi EY: Developmental endothelial locus-1 inhibits MIF production through suppression of NF-κB in macrophages . Int J Mol Med 33: 919-924, 2014.
APA
Lee, S., Kim, D., Kang, Y., Kim, H., Jang, J., Lee, M. ... Choi, E.Y. (2014). Developmental endothelial locus-1 inhibits MIF production through suppression of NF-κB in macrophages . International Journal of Molecular Medicine, 33, 919-924. https://doi.org/10.3892/ijmm.2014.1645
MLA
Lee, S., Kim, D., Kang, Y., Kim, H., Jang, J., Lee, M., Oh, G. T., Kang, S., Choi, E. Y."Developmental endothelial locus-1 inhibits MIF production through suppression of NF-κB in macrophages ". International Journal of Molecular Medicine 33.4 (2014): 919-924.
Chicago
Lee, S., Kim, D., Kang, Y., Kim, H., Jang, J., Lee, M., Oh, G. T., Kang, S., Choi, E. Y."Developmental endothelial locus-1 inhibits MIF production through suppression of NF-κB in macrophages ". International Journal of Molecular Medicine 33, no. 4 (2014): 919-924. https://doi.org/10.3892/ijmm.2014.1645
Copy and paste a formatted citation
x
Spandidos Publications style
Lee S, Kim D, Kang Y, Kim H, Jang J, Lee M, Oh GT, Kang S and Choi EY: Developmental endothelial locus-1 inhibits MIF production through suppression of NF-κB in macrophages . Int J Mol Med 33: 919-924, 2014.
APA
Lee, S., Kim, D., Kang, Y., Kim, H., Jang, J., Lee, M. ... Choi, E.Y. (2014). Developmental endothelial locus-1 inhibits MIF production through suppression of NF-κB in macrophages . International Journal of Molecular Medicine, 33, 919-924. https://doi.org/10.3892/ijmm.2014.1645
MLA
Lee, S., Kim, D., Kang, Y., Kim, H., Jang, J., Lee, M., Oh, G. T., Kang, S., Choi, E. Y."Developmental endothelial locus-1 inhibits MIF production through suppression of NF-κB in macrophages ". International Journal of Molecular Medicine 33.4 (2014): 919-924.
Chicago
Lee, S., Kim, D., Kang, Y., Kim, H., Jang, J., Lee, M., Oh, G. T., Kang, S., Choi, E. Y."Developmental endothelial locus-1 inhibits MIF production through suppression of NF-κB in macrophages ". International Journal of Molecular Medicine 33, no. 4 (2014): 919-924. https://doi.org/10.3892/ijmm.2014.1645
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