Inhibitory effects of Pericarpium zanthoxyli extract on adipocyte differentiation

Kim,Ha-Rim; Kim,Jeong-Mi; Kim,Mi-Seong; Hwang,Jin-Ki; Yang,Sei-Hoon; Kim,Hye-Jung; Lee,Dong-Sung; Oh,Hyuncheol; Kim,Youn-Chul; Ryu,Do-Gon; Lee,Young-Rae; Kwon,Kang-Beom

doi:10.3892/ijmm.2014.1667

May-2014 Volume 33 Issue 5

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May-2014 Volume 33 Issue 5

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Article

Inhibitory effects of Pericarpium zanthoxyli extract on adipocyte differentiation

Authors:
- Ha-Rim Kim
- Jeong-Mi Kim
- Mi-Seong Kim
- Jin-Ki Hwang
- Sei-Hoon Yang
- Hye-Jung Kim
- Dong-Sung Lee
- Hyuncheol Oh
- Youn-Chul Kim
- Do-Gon Ryu
- Young-Rae Lee
- Kang-Beom Kwon
View Affiliations / Copyright

Affiliations: Center for Metabolic Function Regulation, Wonkwang University School of Medicine, Iksan, Jeonbuk 570-749, Republic of Korea, Department of Internal Medicine, Wonkwang University School of Medicine, Iksan, Jeonbuk 570-749, Republic of Korea, Department of Family Medicine, The Catholic University of Korea, Incheon St. Mary's Hospital, Bupyeong-gu, Incheon 403-720, Republic of Korea, Hanbang Body-Fluid Research Center, Wonkwang University, Iksan, Jeonbuk 570-749, Republic of Korea, Department of Korean Physiology, Wonkwang University School of Korean Medicine, Iksan, Jeonbuk 570-749, Republic of Korea
Pages: 1140-1146
|
Published online on: February 25, 2014

https://doi.org/10.3892/ijmm.2014.1667
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Abstract

Obesity is a risk factor associated with numerous disorders, such as type 2 diabetes, hypertension, dyslipidemia and coronary heart disease. In this study, we investigated the inhibitory effects of Pericarpium zanthoxyli extract (PZE) on the adipocytic differentiation of OP9 cells. During adipocyte differentiation, the OP9 cells were treated with 0, 10 and 20 µg/ml of PZE at various time intervals, followed by the examination of lipid droplet formation and the mRNA expression of adipogenesis-related genes. The cells treated with PZE during the early period (days 0-2) showed a significant reduction in the accumulation of lipid droplets, which were induced by a standard adipogenic cocktail, as well as a decrease in the expression of the adipogenesis-related transcription factor, peroxisome proliferator-activated receptor γ (PPARγ) and PPARγ-target genes, such as adipocyte protein 2 (aP2), fatty acid synthase (FAS) and other adipocyte markers. Adipocyte differentiation was not inhibited by treatment with PZE during the late stage of differentiation (days 3-5). Thus, the inhibitory effects of PZE on adipocyte differentiation occurred during the early stages of adipogenesis, which was confirmed by the decrease in the levels of CCAAT/enhancer-binding protein β (C/EBPβ) in a dose-dependent manner when the OP9 cells were exposed to PZE. Taken together, our results indicate that PZE inhibit the early stages of adipogenic differentiation by inhibiting C/EBPβ expression.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Inhibitory effects of Pericarpium zanthoxyli extract on adipocyte differentiation

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