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Activated Wnt signaling induces myofibroblast differentiation of mesenchymal stem cells, contributing to pulmonary fibrosis

  • Authors:
    • Zhaorui Sun
    • Cong Wang
    • Chaowen Shi
    • Fangfang Sun
    • Xiaomeng Xu
    • Weiping Qian
    • Shinan Nie
    • Xiaodong Han
  • View Affiliations / Copyright

    Affiliations: Immunology and Reproductive Biology Laboratory, Medical School of Nanjing University, Nanjing, Jiangsu 210093, P.R. China, State Key Laboratory of Bioelectronics, Southeast University, Nanjing, Jiangsu 210093, P.R. China, Department of Emergency, Jinling Hospital, Medical School of Nanjing University, Nanjing, Jiangsu 210002, P.R. China
    Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 1097-1109
    |
    Published online on: February 25, 2014
       https://doi.org/10.3892/ijmm.2014.1672
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Abstract

Acute lung injury may lead to fibrogenesis. However, no treatment is currently available. This study was conducted to determine the effects of bone marrow-derived mesenchymal stem cells (MSCs) in a model of HCl-induced acute lung injury in Sprague-Dawley (SD) rats. Stromal cell-derived factor (SDF)-1 and its receptor CXC chemokine receptor (CXCR)4 have been shown to participate in mobilizing MSCs. Adenovirus carrying the CXCR4 gene was used to transfect MSCs in order to increase the engraftment numbers of MSCs at injured sites. Histological examination data demonstrated that the engraftment of MSCs did not attenuate lung injury and pulmonary fibrosis. The results showed that engraftment of MSCs almost differentiated into myofibroblasts, but rarely differentiated into lung epithelial cells. Additionally, it was demonstrated that activated canonical Wnt/β-catenin signaling in injured lung tissue regulated the myofibroblast differentiation of MSCs in vivo. The in vitro study results demonstrated that activation of the Wnt/β-catenin signaling stimulated MSCs to express myofibroblast markers; however, this process was attenuated by Wnt antagonist DKK1. Therefore, the results demonstrated that the aberrant activation of Wnt signaling induces the myofibroblast differentiation of engrafted MSCs, thus contributing to pulmonary fibrosis following lung injury.
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Copy and paste a formatted citation
Spandidos Publications style
Sun Z, Wang C, Shi C, Sun F, Xu X, Qian W, Nie S and Han X: Activated Wnt signaling induces myofibroblast differentiation of mesenchymal stem cells, contributing to pulmonary fibrosis. Int J Mol Med 33: 1097-1109, 2014.
APA
Sun, Z., Wang, C., Shi, C., Sun, F., Xu, X., Qian, W. ... Han, X. (2014). Activated Wnt signaling induces myofibroblast differentiation of mesenchymal stem cells, contributing to pulmonary fibrosis. International Journal of Molecular Medicine, 33, 1097-1109. https://doi.org/10.3892/ijmm.2014.1672
MLA
Sun, Z., Wang, C., Shi, C., Sun, F., Xu, X., Qian, W., Nie, S., Han, X."Activated Wnt signaling induces myofibroblast differentiation of mesenchymal stem cells, contributing to pulmonary fibrosis". International Journal of Molecular Medicine 33.5 (2014): 1097-1109.
Chicago
Sun, Z., Wang, C., Shi, C., Sun, F., Xu, X., Qian, W., Nie, S., Han, X."Activated Wnt signaling induces myofibroblast differentiation of mesenchymal stem cells, contributing to pulmonary fibrosis". International Journal of Molecular Medicine 33, no. 5 (2014): 1097-1109. https://doi.org/10.3892/ijmm.2014.1672
Copy and paste a formatted citation
x
Spandidos Publications style
Sun Z, Wang C, Shi C, Sun F, Xu X, Qian W, Nie S and Han X: Activated Wnt signaling induces myofibroblast differentiation of mesenchymal stem cells, contributing to pulmonary fibrosis. Int J Mol Med 33: 1097-1109, 2014.
APA
Sun, Z., Wang, C., Shi, C., Sun, F., Xu, X., Qian, W. ... Han, X. (2014). Activated Wnt signaling induces myofibroblast differentiation of mesenchymal stem cells, contributing to pulmonary fibrosis. International Journal of Molecular Medicine, 33, 1097-1109. https://doi.org/10.3892/ijmm.2014.1672
MLA
Sun, Z., Wang, C., Shi, C., Sun, F., Xu, X., Qian, W., Nie, S., Han, X."Activated Wnt signaling induces myofibroblast differentiation of mesenchymal stem cells, contributing to pulmonary fibrosis". International Journal of Molecular Medicine 33.5 (2014): 1097-1109.
Chicago
Sun, Z., Wang, C., Shi, C., Sun, F., Xu, X., Qian, W., Nie, S., Han, X."Activated Wnt signaling induces myofibroblast differentiation of mesenchymal stem cells, contributing to pulmonary fibrosis". International Journal of Molecular Medicine 33, no. 5 (2014): 1097-1109. https://doi.org/10.3892/ijmm.2014.1672
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