Recombinant growth factor mixtures induce cell cycle progression and the upregulation of type I collagen in human skin fibroblasts, resulting in the acceleration of wound healing processess

Lee,Do Hyun; Choi,Kyung-Ha; Cho,Jae-We; Kim,So Young; Kwon,Tae Rin; Choi,Sun Young; Choi,Yoo Mi; Lee,Jay; Yoon,Ho Sang; Kim,Beom Joon

doi:10.3892/ijmm.2014.1698

May-2014 Volume 33 Issue 5

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May-2014 Volume 33 Issue 5

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Article

Recombinant growth factor mixtures induce cell cycle progression and the upregulation of type I collagen in human skin fibroblasts, resulting in the acceleration of wound healing processess

Authors:
- Do Hyun Lee
- Kyung-Ha Choi
- Jae-We Cho
- So Young Kim
- Tae Rin Kwon
- Sun Young Choi
- Yoo Mi Choi
- Jay Lee
- Ho Sang Yoon
- Beom Joon Kim
View Affiliations / Copyright

Affiliations: Nutrex Technology R&D Center, Seoul, Republic of Korea, Department of Dermatology, Keimyung University School of Medicine, Daegu, Republic of Korea, Department of Convergence Medicine and Pharmaceutical Biosciences, Graduate School, Chung-Ang University, Seoul, Republic of Korea, Department of Dermatology, Chung-Ang University College of Medicine, Seoul, Republic of Korea, Cosmeceutical Team, Pacific Pharmaceuticals, Seoul, Republic of Korea, Marketing and Business Division, Pacific Pharmaceuticals, Seoul, Republic of Korea, MB Business Development Team, Pacific Pharmaceuticals, Seoul, Republic of Korea
Pages: 1147-1152
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Published online on: March 14, 2014

https://doi.org/10.3892/ijmm.2014.1698
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Abstract

Application of growth factor mixtures has been used for wound healing and anti-wrinkles agents. The aim of this study was to evaluate the effect of recombinant growth factor mixtures (RGFM) on the expression of cell cycle regulatory proteins, type I collagen, and wound healing processes of acute animal wound models. The results showed that RGFM induced increased rates of cell proliferation and cell migration of human skin fibroblasts (HSF). In addition, expression of cyclin D1, cyclin E, cyclin-dependent kinase (Cdk)4, and Cdk2 proteins was markedly increased with a growth factor mixtures treatment in fibroblasts. Expression of type I collagen was also increased in growth factor mixtures-treated HSF. Moreover, growth factor mixtures-induced the upregulation of type I collagen was associated with the activation of Smad2/3. In the animal model, RGFM-treated mice showed accelerated wound closure, with the closure rate increasing as early as on day 7, as well as re-epithelization and reduced inflammatory cell infiltration than phosphate-buffered saline (PBS)-treated mice. In conclusion, the results indicated that RGFM has the potential to accelerate wound healing through the upregulation of type I collagen, which is partly mediated by activation of Smad2/3-dependent signaling pathway as well as cell cycle progression in HSF. The topical application of growth factor mixtures to acute and chronic skin wound may accelerate the epithelization process through these molecular mechanisms.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Recombinant growth factor mixtures induce cell cycle progression and the upregulation of type I collagen in human skin fibroblasts, resulting in the acceleration of wound healing processess

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