Recombinant growth factor mixtures induce cell cycle progression and the upregulation of type I collagen in human skin fibroblasts, resulting in the acceleration of wound healing processess

  • Authors:
    • Do Hyun Lee
    • Kyung-Ha Choi
    • Jae-We Cho
    • So Young Kim
    • Tae Rin Kwon
    • Sun Young Choi
    • Yoo Mi Choi
    • Jay Lee
    • Ho Sang Yoon
    • Beom Joon Kim
  • View Affiliations

  • Published online on: March 14, 2014     https://doi.org/10.3892/ijmm.2014.1698
  • Pages: 1147-1152
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Abstract

Application of growth factor mixtures has been used for wound healing and anti-wrinkles agents. The aim of this study was to evaluate the effect of recombinant growth factor mixtures (RGFM) on the expression of cell cycle regulatory proteins, type I collagen, and wound healing processes of acute animal wound models. The results showed that RGFM induced increased rates of cell proliferation and cell migration of human skin fibroblasts (HSF). In addition, expression of cyclin D1, cyclin E, cyclin-dependent kinase (Cdk)4, and Cdk2 proteins was markedly increased with a growth factor mixtures treatment in fibroblasts. Expression of type I collagen was also increased in growth factor mixtures-treated HSF. Moreover, growth factor mixtures-induced the upregulation of type I collagen was associated with the activation of Smad2/3. In the animal model, RGFM-treated mice showed accelerated wound closure, with the closure rate increasing as early as on day 7, as well as re-epithelization and reduced inflammatory cell infiltration than phosphate-buffered saline (PBS)-treated mice. In conclusion, the results indicated that RGFM has the potential to accelerate wound healing through the upregulation of type I collagen, which is partly mediated by activation of Smad2/3-dependent signaling pathway as well as cell cycle progression in HSF. The topical application of growth factor mixtures to acute and chronic skin wound may accelerate the epithelization process through these molecular mechanisms.
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May-2014
Volume 33 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Lee DH, Choi K, Cho J, Kim SY, Kwon TR, Choi SY, Choi YM, Lee J, Yoon HS, Kim BJ, Kim BJ, et al: Recombinant growth factor mixtures induce cell cycle progression and the upregulation of type I collagen in human skin fibroblasts, resulting in the acceleration of wound healing processess. Int J Mol Med 33: 1147-1152, 2014
APA
Lee, D.H., Choi, K., Cho, J., Kim, S.Y., Kwon, T.R., Choi, S.Y. ... Kim, B.J. (2014). Recombinant growth factor mixtures induce cell cycle progression and the upregulation of type I collagen in human skin fibroblasts, resulting in the acceleration of wound healing processess. International Journal of Molecular Medicine, 33, 1147-1152. https://doi.org/10.3892/ijmm.2014.1698
MLA
Lee, D. H., Choi, K., Cho, J., Kim, S. Y., Kwon, T. R., Choi, S. Y., Choi, Y. M., Lee, J., Yoon, H. S., Kim, B. J."Recombinant growth factor mixtures induce cell cycle progression and the upregulation of type I collagen in human skin fibroblasts, resulting in the acceleration of wound healing processess". International Journal of Molecular Medicine 33.5 (2014): 1147-1152.
Chicago
Lee, D. H., Choi, K., Cho, J., Kim, S. Y., Kwon, T. R., Choi, S. Y., Choi, Y. M., Lee, J., Yoon, H. S., Kim, B. J."Recombinant growth factor mixtures induce cell cycle progression and the upregulation of type I collagen in human skin fibroblasts, resulting in the acceleration of wound healing processess". International Journal of Molecular Medicine 33, no. 5 (2014): 1147-1152. https://doi.org/10.3892/ijmm.2014.1698