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International Journal of Molecular Medicine
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Article

Chronic exposure to simulated space conditions predominantly affects cytoskeleton remodeling and oxidative stress response in mouse fetal fibroblasts

  • Authors:
    • Michaël Beck
    • Marjan Moreels
    • Roel Quintens
    • Khalil Abou-El-Ardat
    • Hussein  El-Saghire
    • Kevin Tabury
    • Arlette Michaux
    • Ann Janssen
    • Mieke  Neefs
    • Patrick Van Oostveldt
    • Winnok H. De Vos
    • Sarah Baatout
  • View Affiliations / Copyright

    Affiliations: Radiobiology Unit, Expert Group of Molecular and Cellular Biology, Institute for Environment, Health and Safety, Belgian Nuclear Research Centre (SCK-CEN), Mol, Belgium, Department of Molecular Biotechnology, Ghent University, Ghent, Belgium
  • Pages: 606-615
    |
    Published online on: May 22, 2014
       https://doi.org/10.3892/ijmm.2014.1785
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Abstract

Microgravity and cosmic rays as found in space are difficult to recreate on earth. However, ground-based models exist to simulate space flight experiments. In the present study, an experimental model was utilized to monitor gene expression changes in fetal skin fibroblasts of murine origin. Cells were continuously subjected for 65 h to a low dose (55 mSv) of ionizing radiation (IR), comprising a mixture of high‑linear energy transfer (LET) neutrons and low-LET gamma-rays, and/or simulated microgravity using the random positioning machine (RPM), after which microarrays were performed. The data were analyzed both by gene set enrichment analysis (GSEA) and single gene analysis (SGA). Simulated microgravity affected fetal murine fibroblasts by inducing oxidative stress responsive genes. Three of these genes are targets of the nuclear factor‑erythroid 2 p45-related factor 2 (Nrf2), which may play a role in the cell response to simulated microgravity. In addition, simulated gravity decreased the expression of genes involved in cytoskeleton remodeling, which may have been caused by the downregulation of the serum response factor (SRF), possibly through the Rho signaling pathway. Similarly, chronic exposure to low-dose IR caused the downregulation of genes involved in cytoskeleton remodeling, as well as in cell cycle regulation and DNA damage response pathways. Many of the genes or gene sets that were altered in the individual treatments (RPM or IR) were not altered in the combined treatment (RPM and IR), indicating a complex interaction between RPM and IR.
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Copy and paste a formatted citation
Spandidos Publications style
Beck M, Moreels M, Quintens R, Abou-El-Ardat K, El-Saghire H, Tabury K, Michaux A, Janssen A, Neefs M, Van Oostveldt P, Van Oostveldt P, et al: Chronic exposure to simulated space conditions predominantly affects cytoskeleton remodeling and oxidative stress response in mouse fetal fibroblasts. Int J Mol Med 34: 606-615, 2014.
APA
Beck, M., Moreels, M., Quintens, R., Abou-El-Ardat, K., El-Saghire, H., Tabury, K. ... Baatout, S. (2014). Chronic exposure to simulated space conditions predominantly affects cytoskeleton remodeling and oxidative stress response in mouse fetal fibroblasts. International Journal of Molecular Medicine, 34, 606-615. https://doi.org/10.3892/ijmm.2014.1785
MLA
Beck, M., Moreels, M., Quintens, R., Abou-El-Ardat, K., El-Saghire, H., Tabury, K., Michaux, A., Janssen, A., Neefs, M., Van Oostveldt, P., De Vos, W. H., Baatout, S."Chronic exposure to simulated space conditions predominantly affects cytoskeleton remodeling and oxidative stress response in mouse fetal fibroblasts". International Journal of Molecular Medicine 34.2 (2014): 606-615.
Chicago
Beck, M., Moreels, M., Quintens, R., Abou-El-Ardat, K., El-Saghire, H., Tabury, K., Michaux, A., Janssen, A., Neefs, M., Van Oostveldt, P., De Vos, W. H., Baatout, S."Chronic exposure to simulated space conditions predominantly affects cytoskeleton remodeling and oxidative stress response in mouse fetal fibroblasts". International Journal of Molecular Medicine 34, no. 2 (2014): 606-615. https://doi.org/10.3892/ijmm.2014.1785
Copy and paste a formatted citation
x
Spandidos Publications style
Beck M, Moreels M, Quintens R, Abou-El-Ardat K, El-Saghire H, Tabury K, Michaux A, Janssen A, Neefs M, Van Oostveldt P, Van Oostveldt P, et al: Chronic exposure to simulated space conditions predominantly affects cytoskeleton remodeling and oxidative stress response in mouse fetal fibroblasts. Int J Mol Med 34: 606-615, 2014.
APA
Beck, M., Moreels, M., Quintens, R., Abou-El-Ardat, K., El-Saghire, H., Tabury, K. ... Baatout, S. (2014). Chronic exposure to simulated space conditions predominantly affects cytoskeleton remodeling and oxidative stress response in mouse fetal fibroblasts. International Journal of Molecular Medicine, 34, 606-615. https://doi.org/10.3892/ijmm.2014.1785
MLA
Beck, M., Moreels, M., Quintens, R., Abou-El-Ardat, K., El-Saghire, H., Tabury, K., Michaux, A., Janssen, A., Neefs, M., Van Oostveldt, P., De Vos, W. H., Baatout, S."Chronic exposure to simulated space conditions predominantly affects cytoskeleton remodeling and oxidative stress response in mouse fetal fibroblasts". International Journal of Molecular Medicine 34.2 (2014): 606-615.
Chicago
Beck, M., Moreels, M., Quintens, R., Abou-El-Ardat, K., El-Saghire, H., Tabury, K., Michaux, A., Janssen, A., Neefs, M., Van Oostveldt, P., De Vos, W. H., Baatout, S."Chronic exposure to simulated space conditions predominantly affects cytoskeleton remodeling and oxidative stress response in mouse fetal fibroblasts". International Journal of Molecular Medicine 34, no. 2 (2014): 606-615. https://doi.org/10.3892/ijmm.2014.1785
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