Inhibition by caffeic acid of the influenza A virus multiplication in vitro

Utsunomiya,Hirotoshi; Ichinose,Masao; Ikeda,Keiko; Uozaki,Misao; Morishita,Junko; Kuwahara,Tomomi; Koyama,A.   Hajime; Yamasaki,Hisashi

doi:10.3892/ijmm.2014.1859

October 2014 Volume 34 Issue 4

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October 2014 Volume 34 Issue 4

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Article

Inhibition by caffeic acid of the influenza A virus multiplication in vitro

Authors:
- Hirotoshi Utsunomiya
- Masao Ichinose
- Keiko Ikeda
- Misao Uozaki
- Junko Morishita
- Tomomi Kuwahara
- A. Hajime Koyama
- Hisashi Yamasaki
View Affiliations / Copyright

Affiliations: Department of Strategic Surveillance for Functional Food and Comprehensive Traditional Medicine, Wakayama Medical University Graduate School of Medicine, Wakayama 641-8509, Japan, School of Nursing and Health Science, Wakayama Medical University Graduate School of Medicine, Wakayama 641-8509, Japan, Division of Virology, Department of Cellular and Molecular Medicine, Wakayama Medical University Graduate School of Medicine, Wakayama 641-8509, Japan, Department of Microbiology, Faculty of Medicine, Kagawa University, Kagawa 761-0793, Japan
Pages: 1020-1024
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Published online on: July 22, 2014

https://doi.org/10.3892/ijmm.2014.1859
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Abstract

Caffeic acid has been shown to inhibit the multiplication of influenza A virus in vitro, whereas caffeine, quinic acid and chlorogenic acid do not. Caffeic acid has also been shown to have antiviral activity against herpes simplex virus (DNA virus) and polio virus (RNA virus). In the present study, a comparison of the one-step growth curve of the influenza virus in the presence of caffeic acid with that in the absence of the reagent showed that an eclipse period of the virus multiplication in the infected cells was not affected by the reagent, while the progeny virus yield was markedly decreased in the presence of caffeic acid. In additional experiments, it was found that the addition of caffeic acid at an early time point post-infection (within 3 h post-infection) was mandatory for extensive antiviral activity, suggesting that a major target of the reagent exists in the early stages of infection. Simultaneously with the decrease in the progeny virus yield, both the virus-induced cytopathic effects and apoptotic nuclear fragmentation were markedly suppressed by the reagent, suggesting that caffeic acid suppresses, at least temporally, the degeneration of the virus-infected cells and that the observed antiviral activity is likely not the secondary result of the cytotoxic effects of the reagent. These results suggest the potential pharmacological use of caffeic acid or its derivatives as an antiviral drug against influenza A virus.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Inhibition by caffeic acid of the influenza A virus multiplication in vitro

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Abstract

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