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Curcumin inhibits human retinal pigment epithelial cell proliferation

  • Authors:
    • Yun Sun
    • Zhi-Peng You
  • View Affiliations / Copyright

    Affiliations: Department of Ophthalmology, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China
    Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 1013-1019
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    Published online on: July 23, 2014
       https://doi.org/10.3892/ijmm.2014.1861
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Abstract

Proliferative vitreoretinopathy (PVR) is a common cause of intraoperative failure following retinal reattachment surgery and is mediated in part through the migration, de-differentiation and proliferation of retinal pigment epithelial (RPE) cells. Given the cytotoxic effects of curcumin on epithelial and endothelial cells, in this study, we assessed the effects of curcumin on human RPE (hRPE) cell proliferation. WST-1 analysis revealed that curcumin significantly inhibited primary hRPE cell proliferation in a dose- and time-dependent manner (P<0.001) with the greatest inhibition observed at the dose of 15 µg/ml curcumin. Flow cytometric analysis indicated that the cytotoxic effects of curcumin on hRPE cell proliferation were mediated by cell cycle arrest at the G0/G1 phase and the induction of apoptosis (both P<0.001), which was confirmed by ultrastructural analysis using transmission electron microscopy. Furthermore, western blot analysis revealed that curcumin induced p53 and p21WAF1/CIP1 expression with a concomitant decrease in proliferating cell nuclear antigen protein levels (P<0.05). Curcumin effectively inhibited primary hRPE cell proliferation, which may be mediated by the p53 pathway. Further in vivo studies are required in order to fully explore the therapeutic potential of curcumin for PVR.
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Copy and paste a formatted citation
Spandidos Publications style
Sun Y and You Z: Curcumin inhibits human retinal pigment epithelial cell proliferation. Int J Mol Med 34: 1013-1019, 2014.
APA
Sun, Y., & You, Z. (2014). Curcumin inhibits human retinal pigment epithelial cell proliferation. International Journal of Molecular Medicine, 34, 1013-1019. https://doi.org/10.3892/ijmm.2014.1861
MLA
Sun, Y., You, Z."Curcumin inhibits human retinal pigment epithelial cell proliferation". International Journal of Molecular Medicine 34.4 (2014): 1013-1019.
Chicago
Sun, Y., You, Z."Curcumin inhibits human retinal pigment epithelial cell proliferation". International Journal of Molecular Medicine 34, no. 4 (2014): 1013-1019. https://doi.org/10.3892/ijmm.2014.1861
Copy and paste a formatted citation
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Spandidos Publications style
Sun Y and You Z: Curcumin inhibits human retinal pigment epithelial cell proliferation. Int J Mol Med 34: 1013-1019, 2014.
APA
Sun, Y., & You, Z. (2014). Curcumin inhibits human retinal pigment epithelial cell proliferation. International Journal of Molecular Medicine, 34, 1013-1019. https://doi.org/10.3892/ijmm.2014.1861
MLA
Sun, Y., You, Z."Curcumin inhibits human retinal pigment epithelial cell proliferation". International Journal of Molecular Medicine 34.4 (2014): 1013-1019.
Chicago
Sun, Y., You, Z."Curcumin inhibits human retinal pigment epithelial cell proliferation". International Journal of Molecular Medicine 34, no. 4 (2014): 1013-1019. https://doi.org/10.3892/ijmm.2014.1861
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