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Article

Oxidized S100A4 inhibits the activation of protein phosphatase 5 through S100A1 in MKN‑45 gastric carcinoma cells

  • Authors:
    • Mitsumasa Tsuchiya
    • Fuminori Yamaguchi
    • Seiko Shimamoto
    • Tomohito Fujimoto
    • Hiroshi Tokumitsu
    • Masaaki Tokuda
    • Ryoji Kobayashi
  • View Affiliations / Copyright

    Affiliations: Department of Signal Transduction Sciences, Kagawa University Faculty of Medicine, Kagawa 761‑0793, Japan, Department of Cell Physiology, Kagawa University Faculty of Medicine, Kagawa 761‑0793, Japan
  • Pages: 1713-1719
    |
    Published online on: September 29, 2014
       https://doi.org/10.3892/ijmm.2014.1947
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Abstract

S100 proteins bind to numerous target proteins, as well as other S100 proteins and activate signaling cascades. S100 proteins can be modified by various post‑translational modifications, such as phosphorylation, methylation and acetylation. In addition, oxidation is important for modulating their activities. Previous studies have shown that S100A1 interacts with S100A4 in vitro and in vivo. Due to this potential cross‑talk among the S100 proteins, the aim of the present study was to examine whether S100A4 modulates the activity of S100A1. S100A4 was readily oxidized and formed disulfide‑linked dimers and oligomers. Although non‑oxidized S100A4 bound to protein phosphatase 5 (PP5), the Cu‑oxidized S100A4 failed to bind PP5. Instead, the Cu‑oxidized S100A4 directly interacted with S100A1 and prevented PP5 activation. Hydrogen peroxide induced S100A4 oxidation in MKN‑45 gastric adenocarcinoma cells and decreased S100A1‑PP5 interaction, resulted in the inhibition of PP5 activation by S100A1. These data indicate that oxidized S100A4 regulates PP5 activity in a unique manner under oxidative stress conditions.
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Copy and paste a formatted citation
Spandidos Publications style
Tsuchiya M, Yamaguchi F, Shimamoto S, Fujimoto T, Tokumitsu H, Tokuda M and Kobayashi R: Oxidized S100A4 inhibits the activation of protein phosphatase 5 through S100A1 in MKN‑45 gastric carcinoma cells. Int J Mol Med 34: 1713-1719, 2014.
APA
Tsuchiya, M., Yamaguchi, F., Shimamoto, S., Fujimoto, T., Tokumitsu, H., Tokuda, M., & Kobayashi, R. (2014). Oxidized S100A4 inhibits the activation of protein phosphatase 5 through S100A1 in MKN‑45 gastric carcinoma cells. International Journal of Molecular Medicine, 34, 1713-1719. https://doi.org/10.3892/ijmm.2014.1947
MLA
Tsuchiya, M., Yamaguchi, F., Shimamoto, S., Fujimoto, T., Tokumitsu, H., Tokuda, M., Kobayashi, R."Oxidized S100A4 inhibits the activation of protein phosphatase 5 through S100A1 in MKN‑45 gastric carcinoma cells". International Journal of Molecular Medicine 34.6 (2014): 1713-1719.
Chicago
Tsuchiya, M., Yamaguchi, F., Shimamoto, S., Fujimoto, T., Tokumitsu, H., Tokuda, M., Kobayashi, R."Oxidized S100A4 inhibits the activation of protein phosphatase 5 through S100A1 in MKN‑45 gastric carcinoma cells". International Journal of Molecular Medicine 34, no. 6 (2014): 1713-1719. https://doi.org/10.3892/ijmm.2014.1947
Copy and paste a formatted citation
x
Spandidos Publications style
Tsuchiya M, Yamaguchi F, Shimamoto S, Fujimoto T, Tokumitsu H, Tokuda M and Kobayashi R: Oxidized S100A4 inhibits the activation of protein phosphatase 5 through S100A1 in MKN‑45 gastric carcinoma cells. Int J Mol Med 34: 1713-1719, 2014.
APA
Tsuchiya, M., Yamaguchi, F., Shimamoto, S., Fujimoto, T., Tokumitsu, H., Tokuda, M., & Kobayashi, R. (2014). Oxidized S100A4 inhibits the activation of protein phosphatase 5 through S100A1 in MKN‑45 gastric carcinoma cells. International Journal of Molecular Medicine, 34, 1713-1719. https://doi.org/10.3892/ijmm.2014.1947
MLA
Tsuchiya, M., Yamaguchi, F., Shimamoto, S., Fujimoto, T., Tokumitsu, H., Tokuda, M., Kobayashi, R."Oxidized S100A4 inhibits the activation of protein phosphatase 5 through S100A1 in MKN‑45 gastric carcinoma cells". International Journal of Molecular Medicine 34.6 (2014): 1713-1719.
Chicago
Tsuchiya, M., Yamaguchi, F., Shimamoto, S., Fujimoto, T., Tokumitsu, H., Tokuda, M., Kobayashi, R."Oxidized S100A4 inhibits the activation of protein phosphatase 5 through S100A1 in MKN‑45 gastric carcinoma cells". International Journal of Molecular Medicine 34, no. 6 (2014): 1713-1719. https://doi.org/10.3892/ijmm.2014.1947
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