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International Journal of Molecular Medicine
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The proliferative effects of Pyropia yezoensis peptide on IEC-6 cells are mediated through the epidermal growth factor receptor signaling pathway

  • Authors:
    • Min-Kyeong Lee
    • In-Hye Kim
    • Youn-Hee Choi
    • Jeong-Wook Choi
    • Young-Min Kim
    • Taek-Jeong Nam
  • View Affiliations / Copyright

    Affiliations: Department of Food Science and Nutrition, Pukyong National University, Busan 608-737, Republic of Korea, Institute of Fisheries Science, Pukyong National University, Busan 619-911, Republic of Korea
    Copyright: © Lee et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 909-914
    |
    Published online on: February 26, 2015
       https://doi.org/10.3892/ijmm.2015.2111
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Abstract

For a number of years, seaweed has been used as a functional food in Asian countries, particularly in Korea, Japan and China. Pyropia yezoensis is a marine red alga that has potentially beneficial biological activities. In this study, we examined the mechanisms through which a Pyropia yezoensis peptide [PYP1 (1-20)] induces the proliferation of IEC-6 cells, a rat intestinal epithelial cell line, and the involvement of the epidermal growth factor receptor (EGFR) signaling pathway. First, cell viability assay revealed that PYP1 (1-20) induced cell proliferation in a concentration-dependent manner. Subsequently, we examined the mechanisms responsible for this induction of proliferation induced by PYP1 (1-20). EGFR is widely expressed in mammalian epithelial tissues, and the binding of this ligand affects a variety of cell physiological parameters, such as cell growth and proliferation. PYP1 (1-20) increased the expression of EGFR, Shc, growth factor receptor-bound protein 2 (Grb2) and son of sevenless (SOS). EGFR also induced the activation of the Ras signaling pathway through Raf, MEK and extracellular signal-regulated kinase (ERK) phosphorylation. In addition, cell cycle analysis revealed the expression of cell cycle-related proteins. The results demonstrated an increased number of cells in the G1 phase and an enhanced cell proliferation. In addition, the upregulation of cyclin D, cyclin E, Cdk2, Cdk4 and Cdk6 was observed accompanied by a decreased in p21 and p27 expression. These findings suggest that PYP1 (1-20) stimulates the proliferation of rat IEC-6 cells by activating the EGFR signaling pathway. Therefore, PYP1 (1-20) may be a potential source for the development of bio-functional foods which promotes the proliferation of intestinal epithelial cells.
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Copy and paste a formatted citation
Spandidos Publications style
Lee M, Kim I, Choi Y, Choi J, Kim Y and Nam T: The proliferative effects of Pyropia yezoensis peptide on IEC-6 cells are mediated through the epidermal growth factor receptor signaling pathway. Int J Mol Med 35: 909-914, 2015.
APA
Lee, M., Kim, I., Choi, Y., Choi, J., Kim, Y., & Nam, T. (2015). The proliferative effects of Pyropia yezoensis peptide on IEC-6 cells are mediated through the epidermal growth factor receptor signaling pathway. International Journal of Molecular Medicine, 35, 909-914. https://doi.org/10.3892/ijmm.2015.2111
MLA
Lee, M., Kim, I., Choi, Y., Choi, J., Kim, Y., Nam, T."The proliferative effects of Pyropia yezoensis peptide on IEC-6 cells are mediated through the epidermal growth factor receptor signaling pathway". International Journal of Molecular Medicine 35.4 (2015): 909-914.
Chicago
Lee, M., Kim, I., Choi, Y., Choi, J., Kim, Y., Nam, T."The proliferative effects of Pyropia yezoensis peptide on IEC-6 cells are mediated through the epidermal growth factor receptor signaling pathway". International Journal of Molecular Medicine 35, no. 4 (2015): 909-914. https://doi.org/10.3892/ijmm.2015.2111
Copy and paste a formatted citation
x
Spandidos Publications style
Lee M, Kim I, Choi Y, Choi J, Kim Y and Nam T: The proliferative effects of Pyropia yezoensis peptide on IEC-6 cells are mediated through the epidermal growth factor receptor signaling pathway. Int J Mol Med 35: 909-914, 2015.
APA
Lee, M., Kim, I., Choi, Y., Choi, J., Kim, Y., & Nam, T. (2015). The proliferative effects of Pyropia yezoensis peptide on IEC-6 cells are mediated through the epidermal growth factor receptor signaling pathway. International Journal of Molecular Medicine, 35, 909-914. https://doi.org/10.3892/ijmm.2015.2111
MLA
Lee, M., Kim, I., Choi, Y., Choi, J., Kim, Y., Nam, T."The proliferative effects of Pyropia yezoensis peptide on IEC-6 cells are mediated through the epidermal growth factor receptor signaling pathway". International Journal of Molecular Medicine 35.4 (2015): 909-914.
Chicago
Lee, M., Kim, I., Choi, Y., Choi, J., Kim, Y., Nam, T."The proliferative effects of Pyropia yezoensis peptide on IEC-6 cells are mediated through the epidermal growth factor receptor signaling pathway". International Journal of Molecular Medicine 35, no. 4 (2015): 909-914. https://doi.org/10.3892/ijmm.2015.2111
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