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International Journal of Molecular Medicine
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Article

Peptide derived from desalinated boiled tuna extract inhibits adipogenesis through the downregulation of C/EBP-α and PPAR-γ in 3T3-L1 adipocytes

  • Authors:
    • Young-Min Kim
    • Eun-Young Kim
    • In-Hye Kim
    • Taek-Jeong Nam
  • View Affiliations / Copyright

    Affiliations: Department of Food Science and Nutrition, Pukyong National University, Nam-gu, Busan 608-737, Republic of Korea
  • Pages: 1362-1368
    |
    Published online on: March 6, 2015
       https://doi.org/10.3892/ijmm.2015.2127
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Abstract

Recently, obesity has increased due to a variety of reasons, including the availability of ‘fast food’ and high-fat diets. Developing anti-obesity functional drugs and foods from natural sources may offer solutions to this global concern. Generally, tuna is a high-protein, low-fat and low-calorie food with various bioactive effects. It may improve memory, reduce cholesterol levels and positively affect the development of brain cells. In this study, we screened the anti-obesity potential of peptides derived from tuna protein. We then observed protein bands by the Coomassie blue staining of a sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gel. The protein mixture was concentrated and desalted using in-gel trypsin digestion and a C18 nano column and Poros R2 reversed-phase preparation, prior to quadrupole time-of-flight mass spectrometry (Q-TOF MS/MS). We screened the peptides for their ability to affect adipogenesis in 3T3-L1 adipocytes. We also measured glucose uptake, triglyceride levels and lipid droplets using Oil Red O staining. As a result, we confirmed that one peptide inhibited adipocyte differentiation. We also observed the expression of obesity-related genes by western blot analysis and reverse transcription-polymerase chain reaction. The peptide from the tuna extract significantly reduced the expression levels of CCAAT/enhancer-binding protein α (C/EBP-α) and peroxisome proliferator-activated receptor-γ (PPAR-γ) adipocyte marker genes. Thus, our data suggest that this peptide from boiled tuna extract reduces lipid components and adipogenesis in 3T3-L1 cells, and these characteristics may be of value in the development of anti-obesity foods.
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Copy and paste a formatted citation
Spandidos Publications style
Kim Y, Kim E, Kim I and Nam T: Peptide derived from desalinated boiled tuna extract inhibits adipogenesis through the downregulation of C/EBP-α and PPAR-γ in 3T3-L1 adipocytes. Int J Mol Med 35: 1362-1368, 2015.
APA
Kim, Y., Kim, E., Kim, I., & Nam, T. (2015). Peptide derived from desalinated boiled tuna extract inhibits adipogenesis through the downregulation of C/EBP-α and PPAR-γ in 3T3-L1 adipocytes. International Journal of Molecular Medicine, 35, 1362-1368. https://doi.org/10.3892/ijmm.2015.2127
MLA
Kim, Y., Kim, E., Kim, I., Nam, T."Peptide derived from desalinated boiled tuna extract inhibits adipogenesis through the downregulation of C/EBP-α and PPAR-γ in 3T3-L1 adipocytes". International Journal of Molecular Medicine 35.5 (2015): 1362-1368.
Chicago
Kim, Y., Kim, E., Kim, I., Nam, T."Peptide derived from desalinated boiled tuna extract inhibits adipogenesis through the downregulation of C/EBP-α and PPAR-γ in 3T3-L1 adipocytes". International Journal of Molecular Medicine 35, no. 5 (2015): 1362-1368. https://doi.org/10.3892/ijmm.2015.2127
Copy and paste a formatted citation
x
Spandidos Publications style
Kim Y, Kim E, Kim I and Nam T: Peptide derived from desalinated boiled tuna extract inhibits adipogenesis through the downregulation of C/EBP-α and PPAR-γ in 3T3-L1 adipocytes. Int J Mol Med 35: 1362-1368, 2015.
APA
Kim, Y., Kim, E., Kim, I., & Nam, T. (2015). Peptide derived from desalinated boiled tuna extract inhibits adipogenesis through the downregulation of C/EBP-α and PPAR-γ in 3T3-L1 adipocytes. International Journal of Molecular Medicine, 35, 1362-1368. https://doi.org/10.3892/ijmm.2015.2127
MLA
Kim, Y., Kim, E., Kim, I., Nam, T."Peptide derived from desalinated boiled tuna extract inhibits adipogenesis through the downregulation of C/EBP-α and PPAR-γ in 3T3-L1 adipocytes". International Journal of Molecular Medicine 35.5 (2015): 1362-1368.
Chicago
Kim, Y., Kim, E., Kim, I., Nam, T."Peptide derived from desalinated boiled tuna extract inhibits adipogenesis through the downregulation of C/EBP-α and PPAR-γ in 3T3-L1 adipocytes". International Journal of Molecular Medicine 35, no. 5 (2015): 1362-1368. https://doi.org/10.3892/ijmm.2015.2127
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