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Cellular response of chondrocytes to magnesium alloys for orthopedic applications

  • Authors:
    • Yi Liao
    • Qingli Xu
    • Jian Zhang
    • Jialing Niu
    • Guangyin Yuan
    • Yao Jiang
    • Yaohua He
    • Xinling Wang
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedics, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai 200240, P.R. China, Department of Orthopaedics, The Huashan Hospital Baoshan Branch, Fudan University, Shanghai 200431, P.R. China, National Engineering Research Center of Light Alloys Net Forming (LAF), School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, P.R. China, Department of Orthopaedics, The Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai 200233, P.R. China, Department of Radiology, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai 200240, P.R. China
    Copyright: © Liao et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 73-82
    |
    Published online on: May 14, 2015
       https://doi.org/10.3892/ijmm.2015.2211
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Abstract

In the present study, the effects of Mg-Nd-Zn-Zr (JDBM), brushite (CaHPO4·2H2O)-coated JDBM (C-JDBM), AZ31, WE43, pure magnesium (Mg) and Ti alloy (TC4) on rabbit chondrocytes were investigated in vitro. Adhesion experiments revealed the satisfactory morphology of chondrocytes on the surface of all samples. An indirect cytotoxicity test using MTT assay revealed that C‑JDBM and TC4 exhibited results similar to those of the negative control, better than those obtained with JDBM, AZ31, WE43 and pure Mg (p<0.05). There were no statistically significant differences observed between the JDBM, AZ31, WE43 and pure Mg group (p>0.05). The results of indirect cell cytotoxicity and proliferation assays, as well as those of apoptosis assay, glycosaminoglycan (GAG) quantification, assessment of collagen Ⅱ (Col Ⅱ) levels and RT-qPCR revealed a similar a trend as was observed with MTT assay. These findings suggested that the JDBM alloy was highly biocompatible with chondrocytes in vitro, yielding results similar to those of AZ31, WE43 and pure Mg. Furthermore, CaHPO4·2H2O coating significantly improved the biocompatibility of this alloy.
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Copy and paste a formatted citation
Spandidos Publications style
Liao Y, Xu Q, Zhang J, Niu J, Yuan G, Jiang Y, He Y and Wang X: Cellular response of chondrocytes to magnesium alloys for orthopedic applications. Int J Mol Med 36: 73-82, 2015.
APA
Liao, Y., Xu, Q., Zhang, J., Niu, J., Yuan, G., Jiang, Y. ... Wang, X. (2015). Cellular response of chondrocytes to magnesium alloys for orthopedic applications. International Journal of Molecular Medicine, 36, 73-82. https://doi.org/10.3892/ijmm.2015.2211
MLA
Liao, Y., Xu, Q., Zhang, J., Niu, J., Yuan, G., Jiang, Y., He, Y., Wang, X."Cellular response of chondrocytes to magnesium alloys for orthopedic applications". International Journal of Molecular Medicine 36.1 (2015): 73-82.
Chicago
Liao, Y., Xu, Q., Zhang, J., Niu, J., Yuan, G., Jiang, Y., He, Y., Wang, X."Cellular response of chondrocytes to magnesium alloys for orthopedic applications". International Journal of Molecular Medicine 36, no. 1 (2015): 73-82. https://doi.org/10.3892/ijmm.2015.2211
Copy and paste a formatted citation
x
Spandidos Publications style
Liao Y, Xu Q, Zhang J, Niu J, Yuan G, Jiang Y, He Y and Wang X: Cellular response of chondrocytes to magnesium alloys for orthopedic applications. Int J Mol Med 36: 73-82, 2015.
APA
Liao, Y., Xu, Q., Zhang, J., Niu, J., Yuan, G., Jiang, Y. ... Wang, X. (2015). Cellular response of chondrocytes to magnesium alloys for orthopedic applications. International Journal of Molecular Medicine, 36, 73-82. https://doi.org/10.3892/ijmm.2015.2211
MLA
Liao, Y., Xu, Q., Zhang, J., Niu, J., Yuan, G., Jiang, Y., He, Y., Wang, X."Cellular response of chondrocytes to magnesium alloys for orthopedic applications". International Journal of Molecular Medicine 36.1 (2015): 73-82.
Chicago
Liao, Y., Xu, Q., Zhang, J., Niu, J., Yuan, G., Jiang, Y., He, Y., Wang, X."Cellular response of chondrocytes to magnesium alloys for orthopedic applications". International Journal of Molecular Medicine 36, no. 1 (2015): 73-82. https://doi.org/10.3892/ijmm.2015.2211
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