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miR-21 synergizes with BMP9 in osteogenic differentiation by activating the BMP9/Smad signaling pathway in murine multilineage cells

  • Authors:
    • Qiling Song
    • Liang Zhong
    • Chu Chen
    • Zuchuan Tang
    • Hongxia Liu
    • Yiqin Zhou
    • Min Tang
    • Lan Zhou
    • Guowei Zuo
    • Jinyong Luo
    • Yan Zhang
    • Qiong Shi
    • Yaguang Weng
  • View Affiliations / Copyright

    Affiliations: Key Laboratory of Diagnostic Medicine designated by the Chinese Ministry of Education, Chongqing Medical University, Chongqing 400016, P.R. China
    Copyright: © Song et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1497-1506
    |
    Published online on: October 9, 2015
       https://doi.org/10.3892/ijmm.2015.2363
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Abstract

Bone morphogenetic proteins (BMPs), particularly BMP9, have been shown to promote the osteogenic differentiation of murine multilineage cells (MMCs) and to promote bone formation in bone diseases; however, the mechanisms involved remain poorly understood. MicroRNAs (miRNAs or miRs) have been proven to regulate mesenchymal stem cell (MSC) differentiation. In this study, we identified a novel mechanism that unravels the functional axis of a key miRNA (miR-21) which contributes to BMP9‑induced osteogenic differentiation. We screened differentially expressed miRNAs in MMCs during BMP9‑induced osteogenic differentiation and found that miR-21 was significantly upregulated by BMP9 during the osteogenesis of MMCs. Furthermore, miR-21 was confirmed to promote the osteogenic differentiation of the MMCs by suppressing Smad7, which negatively regulates the osteogenic differentiation of MMCs. The upregulation of miR-21 may promote the osteogenic differentiation of MMCs in synergy with BMP9. The findings of our study revealed a novel function of miR-21, and suggest that the overexpression of miR-21 contributes to bone formation by promoting BMP9‑induced osteogenic differentiation. Our data may provide a molecular basis for the development of novel therapeutic strategies to treat bone diseases, such as osteoporosis and other inflammatory bone diseases.
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Copy and paste a formatted citation
Spandidos Publications style
Song Q, Zhong L, Chen C, Tang Z, Liu H, Zhou Y, Tang M, Zhou L, Zuo G, Luo J, Luo J, et al: miR-21 synergizes with BMP9 in osteogenic differentiation by activating the BMP9/Smad signaling pathway in murine multilineage cells. Int J Mol Med 36: 1497-1506, 2015.
APA
Song, Q., Zhong, L., Chen, C., Tang, Z., Liu, H., Zhou, Y. ... Weng, Y. (2015). miR-21 synergizes with BMP9 in osteogenic differentiation by activating the BMP9/Smad signaling pathway in murine multilineage cells. International Journal of Molecular Medicine, 36, 1497-1506. https://doi.org/10.3892/ijmm.2015.2363
MLA
Song, Q., Zhong, L., Chen, C., Tang, Z., Liu, H., Zhou, Y., Tang, M., Zhou, L., Zuo, G., Luo, J., Zhang, Y., Shi, Q., Weng, Y."miR-21 synergizes with BMP9 in osteogenic differentiation by activating the BMP9/Smad signaling pathway in murine multilineage cells". International Journal of Molecular Medicine 36.6 (2015): 1497-1506.
Chicago
Song, Q., Zhong, L., Chen, C., Tang, Z., Liu, H., Zhou, Y., Tang, M., Zhou, L., Zuo, G., Luo, J., Zhang, Y., Shi, Q., Weng, Y."miR-21 synergizes with BMP9 in osteogenic differentiation by activating the BMP9/Smad signaling pathway in murine multilineage cells". International Journal of Molecular Medicine 36, no. 6 (2015): 1497-1506. https://doi.org/10.3892/ijmm.2015.2363
Copy and paste a formatted citation
x
Spandidos Publications style
Song Q, Zhong L, Chen C, Tang Z, Liu H, Zhou Y, Tang M, Zhou L, Zuo G, Luo J, Luo J, et al: miR-21 synergizes with BMP9 in osteogenic differentiation by activating the BMP9/Smad signaling pathway in murine multilineage cells. Int J Mol Med 36: 1497-1506, 2015.
APA
Song, Q., Zhong, L., Chen, C., Tang, Z., Liu, H., Zhou, Y. ... Weng, Y. (2015). miR-21 synergizes with BMP9 in osteogenic differentiation by activating the BMP9/Smad signaling pathway in murine multilineage cells. International Journal of Molecular Medicine, 36, 1497-1506. https://doi.org/10.3892/ijmm.2015.2363
MLA
Song, Q., Zhong, L., Chen, C., Tang, Z., Liu, H., Zhou, Y., Tang, M., Zhou, L., Zuo, G., Luo, J., Zhang, Y., Shi, Q., Weng, Y."miR-21 synergizes with BMP9 in osteogenic differentiation by activating the BMP9/Smad signaling pathway in murine multilineage cells". International Journal of Molecular Medicine 36.6 (2015): 1497-1506.
Chicago
Song, Q., Zhong, L., Chen, C., Tang, Z., Liu, H., Zhou, Y., Tang, M., Zhou, L., Zuo, G., Luo, J., Zhang, Y., Shi, Q., Weng, Y."miR-21 synergizes with BMP9 in osteogenic differentiation by activating the BMP9/Smad signaling pathway in murine multilineage cells". International Journal of Molecular Medicine 36, no. 6 (2015): 1497-1506. https://doi.org/10.3892/ijmm.2015.2363
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