Bioactive peptide from Pyropia yezoensis and its anti-inflammatory activities

Lee,Hyun‑Ah; Kim,In‑Hye; Nam,Taek‑Jeong

doi:10.3892/ijmm.2015.2386

Bioactive peptide from Pyropia yezoensis and its anti-inflammatory activities

Authors:
- Hyun‑Ah Lee
- In‑Hye Kim
- Taek‑Jeong Nam
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Affiliations: Institute of Fisheries Sciences, Pukyong National University, Busan 619‑911, Republic of Korea
Published online on: October 21, 2015 https://doi.org/10.3892/ijmm.2015.2386
Pages: 1701-1706

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Abstract

Pyropia yezoensis (P. yezoensis) is an important marine algae. Its high protein content serves as a good source of biologically active peptides. Potent inhibitory effects on the production of inflammatory mediators were observed in a bioactive peptide derived from P. yezoensis (peptide from P. yezoensis; PPY1), as demonstrated in lipopolysaccharide (LPS)‑stimulated macrophages. The present study showed that peptide concentrations ranging from 250 to 1,000 ng/ml had no significant cytotoxicity in the cell viability assay when applied to the RAW 264.7 cells for 24 h. PPY1 completely inhibited LPS‑stimulated nitric oxide (NO) release in a dose‑dependent manner. Fluorescence intensity, corresponding to intracellular reactive oxygen species (ROS) produced by 10 ng/ml LPS‑stimulated cells, significantly shifted, indicating that the peptide reduced the level of ROS. Furthermore, PPY1 exerted potent inhibitory activity to reduce the release of pro‑inflammatory cytokines (inducible NO synthase, cyclooxygenase‑2, interleukin‑1β and tumor necrosis factor‑α) in LPS‑stimulated macrophages in a dose‑dependent manner. These results also showed that the anti‑inflammatory activity of PPY1 was associated with downregulation of extracellular signal‑regulated kinase, protein 38, and c‑jun NH2‑terminal kinase phosphorylation in the mitogen‑activated protein kinase pathways. In conclusion, PPY1 can have a significant role as an anti‑inflammatory agent, with a potential for use in marine products.

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