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Article

Osthole ameliorates acute myocardial infarction in rats by decreasing the expression of inflammatory-related cytokines, diminishing MMP-2 expression and activating p-ERK

  • Authors:
    • Juan Duan
    • Yu Yang
    • Hong Liu
    • Peng-Cheng Dou
    • Sheng-Yu Tan
  • View Affiliations / Copyright

    Affiliations: Department of Geriatrics, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, P.R. China, Department of Orthopedics, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, P.R. China
  • Pages: 207-216
    |
    Published online on: November 4, 2015
       https://doi.org/10.3892/ijmm.2015.2402
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Abstract

Osthole, the active constituent of Cnidium monnieri extracts, has been shown to have a diverse range of pharmacological properties. In the present study, we aimed to evaluate the cardioprotective effects of osthole in a rat model of acute myocardial infarction (AMI). The rats with AMI were treated with 1, 3 and 10 mg/kg of osthole or the vehicle for 4 weeks. The infarct size of the rats with AMI was measured, and casein kinase (CK), the MB isoenzyme of creatine kinase (CK-MB), lactate dehydrogenase (LDH) and cardiac troponin T (cTnT) activities in the rats with AMI were analyzed using commercially available kits. The nuclear factor-κB (NF-κB), tumor necrosis factor‑α (TNF-α), interleukin (IL)-1β and IL-6 levels in whole blood from rats with AMI were also detected using commercially available kits. The levels of Toll-like receptors 2/4 (TLR2/4) and nucleotide-binding oligomerization domain-containing protein 1/2 (NOD1/2) were also detected by RT-qPCR. Moreover, the protein expression levels of endothelial nitric oxide synthase (eNOS) and mitogen-activated protein kinase (MAPK) cascades, including extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK) and p38, cyclooxygenase-2 (COX-2), as well as matrix metalloproteinase-2 (MMP-2) were all assayed by western blot analysis. Our results revealed that osthole markedly reduced the infarct size, and the levels of CK, CK-MB, LDH and cTnT in the rats with AMI, and that these cardioprotective effects may be associated with the inhibition of inflammatory reactions, the reduction in MMP-2 activity and the activation of MAPK cascades.
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Copy and paste a formatted citation
Spandidos Publications style
Duan J, Yang Y, Liu H, Dou P and Tan S: Osthole ameliorates acute myocardial infarction in rats by decreasing the expression of inflammatory-related cytokines, diminishing MMP-2 expression and activating p-ERK. Int J Mol Med 37: 207-216, 2016.
APA
Duan, J., Yang, Y., Liu, H., Dou, P., & Tan, S. (2016). Osthole ameliorates acute myocardial infarction in rats by decreasing the expression of inflammatory-related cytokines, diminishing MMP-2 expression and activating p-ERK. International Journal of Molecular Medicine, 37, 207-216. https://doi.org/10.3892/ijmm.2015.2402
MLA
Duan, J., Yang, Y., Liu, H., Dou, P., Tan, S."Osthole ameliorates acute myocardial infarction in rats by decreasing the expression of inflammatory-related cytokines, diminishing MMP-2 expression and activating p-ERK". International Journal of Molecular Medicine 37.1 (2016): 207-216.
Chicago
Duan, J., Yang, Y., Liu, H., Dou, P., Tan, S."Osthole ameliorates acute myocardial infarction in rats by decreasing the expression of inflammatory-related cytokines, diminishing MMP-2 expression and activating p-ERK". International Journal of Molecular Medicine 37, no. 1 (2016): 207-216. https://doi.org/10.3892/ijmm.2015.2402
Copy and paste a formatted citation
x
Spandidos Publications style
Duan J, Yang Y, Liu H, Dou P and Tan S: Osthole ameliorates acute myocardial infarction in rats by decreasing the expression of inflammatory-related cytokines, diminishing MMP-2 expression and activating p-ERK. Int J Mol Med 37: 207-216, 2016.
APA
Duan, J., Yang, Y., Liu, H., Dou, P., & Tan, S. (2016). Osthole ameliorates acute myocardial infarction in rats by decreasing the expression of inflammatory-related cytokines, diminishing MMP-2 expression and activating p-ERK. International Journal of Molecular Medicine, 37, 207-216. https://doi.org/10.3892/ijmm.2015.2402
MLA
Duan, J., Yang, Y., Liu, H., Dou, P., Tan, S."Osthole ameliorates acute myocardial infarction in rats by decreasing the expression of inflammatory-related cytokines, diminishing MMP-2 expression and activating p-ERK". International Journal of Molecular Medicine 37.1 (2016): 207-216.
Chicago
Duan, J., Yang, Y., Liu, H., Dou, P., Tan, S."Osthole ameliorates acute myocardial infarction in rats by decreasing the expression of inflammatory-related cytokines, diminishing MMP-2 expression and activating p-ERK". International Journal of Molecular Medicine 37, no. 1 (2016): 207-216. https://doi.org/10.3892/ijmm.2015.2402
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