Anti-allergic effects of a nonameric peptide isolated from the intestine gastrointestinal digests of abalone (Haliotis discus hannai) in activated HMC-1 human mast cells

  • Authors:
    • Seok-Chun Ko
    • Dae-Sung Lee
    • Won Sun Park
    • Jong Su Yoo
    • Mi-Jin Yim
    • Zhong-Ji Qian
    • Chang-Min Lee
    • Junghwan Oh
    • Won-Kyo Jung
    • Il-Whan Choi
  • View Affiliations

  • Published online on: November 27, 2015     https://doi.org/10.3892/ijmm.2015.2420
  • Pages: 243-250
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The aim of the present study was to examine whether the intestine gastrointestinal (GI) digests of abalone [Haliotis discus hannai (H. discus hannai)] modulate inflammatory responses and to elucidate the mechanisms involved. The GI digests of the abalone intestines were fractionated into fractions I (>10 kDa), II (5-10 kDa) and Ⅲ (<5 kDa). Of the abalone intestine GI digests (AIGIDs), fraction Ⅲ inhibited the passive cutaneous anaphylaxis (PCA) reaction in mice. Subsequently, a bioactive peptide [abalone intestine GI digest peptide (AIGIDP)] isolated from fraction Ⅲ was determined to be 1175.2 Da, and the amino acid sequence was found to be PFNQGTFAS. We noted that the purified nonameric peptide (AIGIDP) attenuated the phorbol‑12‑myristate 13-acetate plus calcium ionophore A23187 (PMACI)-induced histamine release and the production of pro-inflammatory cytokines, such as tumor necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6 in human mast cells (HMC-1 cells). In addition, we also noted that AIGIDP inhibited the PMACI‑induced activation of nuclear factor‑κB (NF-κB) by suppressing IκBα phosphorylation and that it suppressed the production of cytokines by decreasing the phosphorylation of JNK. The findings of our study indicate that AIGIDP exerts a modulatory, anti-allergic effect on mast cell-mediated inflammatory diseases.
View Figures
View References

Related Articles

Journal Cover

January-2016
Volume 37 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Ko S, Lee D, Park WS, Yoo JS, Yim M, Qian Z, Lee C, Oh J, Jung W, Choi I, Choi I, et al: Anti-allergic effects of a nonameric peptide isolated from the intestine gastrointestinal digests of abalone (Haliotis discus hannai) in activated HMC-1 human mast cells. Int J Mol Med 37: 243-250, 2016.
APA
Ko, S., Lee, D., Park, W.S., Yoo, J.S., Yim, M., Qian, Z. ... Choi, I. (2016). Anti-allergic effects of a nonameric peptide isolated from the intestine gastrointestinal digests of abalone (Haliotis discus hannai) in activated HMC-1 human mast cells. International Journal of Molecular Medicine, 37, 243-250. https://doi.org/10.3892/ijmm.2015.2420
MLA
Ko, S., Lee, D., Park, W. S., Yoo, J. S., Yim, M., Qian, Z., Lee, C., Oh, J., Jung, W., Choi, I."Anti-allergic effects of a nonameric peptide isolated from the intestine gastrointestinal digests of abalone (Haliotis discus hannai) in activated HMC-1 human mast cells". International Journal of Molecular Medicine 37.1 (2016): 243-250.
Chicago
Ko, S., Lee, D., Park, W. S., Yoo, J. S., Yim, M., Qian, Z., Lee, C., Oh, J., Jung, W., Choi, I."Anti-allergic effects of a nonameric peptide isolated from the intestine gastrointestinal digests of abalone (Haliotis discus hannai) in activated HMC-1 human mast cells". International Journal of Molecular Medicine 37, no. 1 (2016): 243-250. https://doi.org/10.3892/ijmm.2015.2420