A fraction from Dojuksan 30% ethanol extract exerts its anti-inflammatory effects through Nrf2-dependent heme oxygenase-1 expression

  • Authors:
    • Dong-Sung Lee
    • Kyoung-Su Kim
    • Wonmin Ko
    • Gi-Sang Bae
    • Sung-Joo Park
    • Jun-Hyeog Jang
    • Hyuncheol Oh
    • Youn-Chul Kim
  • View Affiliations

  • Published online on: December 7, 2015     https://doi.org/10.3892/ijmm.2015.2424
  • Pages: 475-484
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Abstract

Dojuksan is a traditional herbal medicine used in Korea and China to treat urinary diseases. In the present study, we aimed to examine the anti-inflammatory effects of an ethanol solvent extract of Dojuksan and a fraction (by bioassay-guided fractionation) derived from this extract, and to elucidate the specific mechanisms involved. The Dojuksan 30% ethanol extract (DEE) had a more significant and potent anti-inflammatory effect than the Dojuksan water extract (DWE). DEE markedly inhibited the production of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), nitric oxide (NO), prostaglandin E2 (PGE2), tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β), as well as nuclear factor-κB (NF-κB) binding activity. We found that the anti-inflammatory effects of DEE were mediated by the induction of nuclear factor E2-related factor 2 (Nrf2)-dependent heme oxygenase-1 (HO-1). To further explore the anti-inflammatory effects of DEE, we generated 6 different fractions of DEE. Of these, DEE-5 decreased the production of NO more significantly than the other fractions. DEE-5 also significantly decreased the expression of iNOS and COX-2, and the production of NO, PGE2, TNF-α and IL-1β. In addition, DEE-5 also significantly increased HO-1 levels; HO-1 significanlty contributed to the inhibitory effects of DEE-5 on the production of pro-inflammatory mediators. In this study, we determined whether the choice of extraction solvent affects the biological activity of Dojuksan, a traditional herbal formula. Our findings demonstrate that DEE and a fraction derived from this extract exerts anti-inflammatory effects through Nrf2‑dependent HO-1 expression, and that DEE may thus have greater potential therapeutic application than DWE.
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February-2016
Volume 37 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Lee D, Kim K, Ko W, Bae G, Park S, Jang J, Oh H and Kim Y: A fraction from Dojuksan 30% ethanol extract exerts its anti-inflammatory effects through Nrf2-dependent heme oxygenase-1 expression. Int J Mol Med 37: 475-484, 2016.
APA
Lee, D., Kim, K., Ko, W., Bae, G., Park, S., Jang, J. ... Kim, Y. (2016). A fraction from Dojuksan 30% ethanol extract exerts its anti-inflammatory effects through Nrf2-dependent heme oxygenase-1 expression. International Journal of Molecular Medicine, 37, 475-484. https://doi.org/10.3892/ijmm.2015.2424
MLA
Lee, D., Kim, K., Ko, W., Bae, G., Park, S., Jang, J., Oh, H., Kim, Y."A fraction from Dojuksan 30% ethanol extract exerts its anti-inflammatory effects through Nrf2-dependent heme oxygenase-1 expression". International Journal of Molecular Medicine 37.2 (2016): 475-484.
Chicago
Lee, D., Kim, K., Ko, W., Bae, G., Park, S., Jang, J., Oh, H., Kim, Y."A fraction from Dojuksan 30% ethanol extract exerts its anti-inflammatory effects through Nrf2-dependent heme oxygenase-1 expression". International Journal of Molecular Medicine 37, no. 2 (2016): 475-484. https://doi.org/10.3892/ijmm.2015.2424