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Article

Inhibitory effect of fermented Arctium lappa fruit extract on the IgE-mediated allergic response in RBL‑2H3 cells

  • Authors:
    • Jae-Myung Yoo
    • Ju Hye Yang
    • Hye Jin Yang
    • Won-Kyung Cho
    • Jin Yeul Ma
  • View Affiliations / Copyright

    Affiliations: Korean Medicine (KM)-Application Center, Korea Institute of Oriental Medicine (KIOM), Daegu 41062, Republic of Korea
  • Pages: 501-508
    |
    Published online on: December 28, 2015
       https://doi.org/10.3892/ijmm.2015.2447
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Abstract

Arctium lappa fruit has been used in traditional medicine, and it is known to exert beneficial effects, such as antioxidant, anti-inflammatory and anticancer effects. However, the effects of the Arctium lappa fruit on the allergic response remain unknown. In this study, we evaluated the anti-allergic effects of Arctium lappa fruit extract (AFE) and its fermented form (F-AFE) using immunoglobulin E (IgE)-activated RBL‑2H3 cells. To investigate the anti-allergic effects of AFE or F-AFE, we examined the release of β-hexosaminidase, a key biomarker of degranulation during an allergic reaction, and the production of pro-inflammatory mediators, such as tumor necrosis factor-α (TNF-α) and prostaglandin E2 (PGE2) in the cells treated with or without the above-mentioned extracts. AFE weakly inhibited the release of β-hexosaminidase, whereas F-AFE significantly suppressed the release of β-hexosaminidase in a dose-dependent manner. Consistently, F-AFE suppressed the production of TNF-α and PGE2 in a dose-dependent manner. F-AFE exerted an inhibitory effect on the production of β-hexosaminidase, TNF-α and PGE2 with an IC50 value of 30.73, 46.96 and 36.27 µg/ml, respectively. Furthermore, F-AFE inhibited the phosphorylation of Lyn, Fyn and Syk, which are involved in the FcεRI signaling pathway, that of phosphoinositide phospholipase C (PLC)γ1/2 and protein kinase C (PKC)δ, which are associated with the degranulation process, as well as that of extracellular signal-regulated kinase 1/2 (ERK1/2), c-Jun N-terminal kinase 1/2 (JNK), p38 and Akt, which are associated with cytokine expression. In the late phase, F-AFE partially suppressed the phosphorylation of cytosolic phospholipase A2 (cPLA2), but not the expression of cyclooxygenase (COX)-2. To compare and identify the major components of the two extracts, we used high-performance liquid chromatography. The levels of arctigenin, one of the major compounds, were elevated 6-fold in F-AFE compared with AFE, whereas the levels of arctiin, an arctigenin glycoside, were decreased in F-AFE by approximately 57.40%. These results suggest that arctigenin plays an important role in the anti-allergic effects of F-AFE. Taken together, F-AFE containing anti-allergic phytochemicals, including arctigenin, inhibited the activation of the FcεRI receptor induced by the antigen‑IgE complex. Such effects may provide further information for the development of a phytomedicine for allergic diseases.
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Copy and paste a formatted citation
Spandidos Publications style
Yoo J, Yang JH, Yang HJ, Cho W and Ma JY: Inhibitory effect of fermented Arctium lappa fruit extract on the IgE-mediated allergic response in RBL‑2H3 cells. Int J Mol Med 37: 501-508, 2016.
APA
Yoo, J., Yang, J.H., Yang, H.J., Cho, W., & Ma, J.Y. (2016). Inhibitory effect of fermented Arctium lappa fruit extract on the IgE-mediated allergic response in RBL‑2H3 cells. International Journal of Molecular Medicine, 37, 501-508. https://doi.org/10.3892/ijmm.2015.2447
MLA
Yoo, J., Yang, J. H., Yang, H. J., Cho, W., Ma, J. Y."Inhibitory effect of fermented Arctium lappa fruit extract on the IgE-mediated allergic response in RBL‑2H3 cells". International Journal of Molecular Medicine 37.2 (2016): 501-508.
Chicago
Yoo, J., Yang, J. H., Yang, H. J., Cho, W., Ma, J. Y."Inhibitory effect of fermented Arctium lappa fruit extract on the IgE-mediated allergic response in RBL‑2H3 cells". International Journal of Molecular Medicine 37, no. 2 (2016): 501-508. https://doi.org/10.3892/ijmm.2015.2447
Copy and paste a formatted citation
x
Spandidos Publications style
Yoo J, Yang JH, Yang HJ, Cho W and Ma JY: Inhibitory effect of fermented Arctium lappa fruit extract on the IgE-mediated allergic response in RBL‑2H3 cells. Int J Mol Med 37: 501-508, 2016.
APA
Yoo, J., Yang, J.H., Yang, H.J., Cho, W., & Ma, J.Y. (2016). Inhibitory effect of fermented Arctium lappa fruit extract on the IgE-mediated allergic response in RBL‑2H3 cells. International Journal of Molecular Medicine, 37, 501-508. https://doi.org/10.3892/ijmm.2015.2447
MLA
Yoo, J., Yang, J. H., Yang, H. J., Cho, W., Ma, J. Y."Inhibitory effect of fermented Arctium lappa fruit extract on the IgE-mediated allergic response in RBL‑2H3 cells". International Journal of Molecular Medicine 37.2 (2016): 501-508.
Chicago
Yoo, J., Yang, J. H., Yang, H. J., Cho, W., Ma, J. Y."Inhibitory effect of fermented Arctium lappa fruit extract on the IgE-mediated allergic response in RBL‑2H3 cells". International Journal of Molecular Medicine 37, no. 2 (2016): 501-508. https://doi.org/10.3892/ijmm.2015.2447
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