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International Journal of Molecular Medicine
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Article Open Access

Re-infection of the prion from the scrapie‑infected cell line SMB-S15 in three strains of mice, CD1, C57BL/6 and Balb/c

  • Authors:
    • Kang Xiao
    • Bao-Yun Zhang
    • Xiao-Mei Zhang
    • Jing Wang
    • Cao Chen
    • Li-Na Chen
    • Yan Lv
    • Qi Shi
    • Xiao-Ping Dong
  • View Affiliations / Copyright

    Affiliations: State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases of Zhejiang University, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, P.R. China
    Copyright: © Xiao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 716-726
    |
    Published online on: January 25, 2016
       https://doi.org/10.3892/ijmm.2016.2465
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Abstract

It is well known that the SMB-S15 cell line was originally established by cultures from the brains of mice affected by the Chandler scrapie strain, and this cell line may express PrPSc permanently. However, the infectivity of the S15-derived prions on experimental animals has not yet been well documented. In the present study, the cell lysates of SMB-S15 were intracerebrally inoculated into three different strains of mice, namely C57BL/6, Balb/c and CD1. Prion protein (PRNP) gene sequencing revealed the same encoded PrP proteins in the sequences of amino acids in the three strains of mice, in addition to a synonymous single nucleotide polymorphism (SNP) in CD1 mice. All infected mice developed typical experimental transmissible spongiform encephalopathies (TSEs) approximately six months post-infection. The clinical features of three infected mice were comparable. The pathogenic characteristics, such as the electrophoretic and glycosylation profiles and proteinase K (PK) resistance of PrPSc molecules, as well as the neuropathological characteristics, such as spongiform vacuolation, PrPSc deposits in cortex regions, astrogliosis and activated microglia, were also similar in all three strains of infected mice. However, PrPSc deposits in the cerebellums of CD1 mice were significantly fewer, which was linked with the observation that lower numbers of CD1 mice presented cerebellum-associated symptoms. Successive inoculation of the individual strains of mice with brain homogenates from the infected mice also induced typical experimental scrapie. The data in the present study thus confirm that the prion agent in SMB-S15 cells causes stable infectivity in different types of mice with distinct phenotypes after long-term propagation in vitro. The present study also provides further scrapie rodent models, which may be used in further studies.
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Copy and paste a formatted citation
Spandidos Publications style
Xiao K, Zhang B, Zhang X, Wang J, Chen C, Chen L, Lv Y, Shi Q and Dong X: Re-infection of the prion from the scrapie‑infected cell line SMB-S15 in three strains of mice, CD1, C57BL/6 and Balb/c. Int J Mol Med 37: 716-726, 2016.
APA
Xiao, K., Zhang, B., Zhang, X., Wang, J., Chen, C., Chen, L. ... Dong, X. (2016). Re-infection of the prion from the scrapie‑infected cell line SMB-S15 in three strains of mice, CD1, C57BL/6 and Balb/c. International Journal of Molecular Medicine, 37, 716-726. https://doi.org/10.3892/ijmm.2016.2465
MLA
Xiao, K., Zhang, B., Zhang, X., Wang, J., Chen, C., Chen, L., Lv, Y., Shi, Q., Dong, X."Re-infection of the prion from the scrapie‑infected cell line SMB-S15 in three strains of mice, CD1, C57BL/6 and Balb/c". International Journal of Molecular Medicine 37.3 (2016): 716-726.
Chicago
Xiao, K., Zhang, B., Zhang, X., Wang, J., Chen, C., Chen, L., Lv, Y., Shi, Q., Dong, X."Re-infection of the prion from the scrapie‑infected cell line SMB-S15 in three strains of mice, CD1, C57BL/6 and Balb/c". International Journal of Molecular Medicine 37, no. 3 (2016): 716-726. https://doi.org/10.3892/ijmm.2016.2465
Copy and paste a formatted citation
x
Spandidos Publications style
Xiao K, Zhang B, Zhang X, Wang J, Chen C, Chen L, Lv Y, Shi Q and Dong X: Re-infection of the prion from the scrapie‑infected cell line SMB-S15 in three strains of mice, CD1, C57BL/6 and Balb/c. Int J Mol Med 37: 716-726, 2016.
APA
Xiao, K., Zhang, B., Zhang, X., Wang, J., Chen, C., Chen, L. ... Dong, X. (2016). Re-infection of the prion from the scrapie‑infected cell line SMB-S15 in three strains of mice, CD1, C57BL/6 and Balb/c. International Journal of Molecular Medicine, 37, 716-726. https://doi.org/10.3892/ijmm.2016.2465
MLA
Xiao, K., Zhang, B., Zhang, X., Wang, J., Chen, C., Chen, L., Lv, Y., Shi, Q., Dong, X."Re-infection of the prion from the scrapie‑infected cell line SMB-S15 in three strains of mice, CD1, C57BL/6 and Balb/c". International Journal of Molecular Medicine 37.3 (2016): 716-726.
Chicago
Xiao, K., Zhang, B., Zhang, X., Wang, J., Chen, C., Chen, L., Lv, Y., Shi, Q., Dong, X."Re-infection of the prion from the scrapie‑infected cell line SMB-S15 in three strains of mice, CD1, C57BL/6 and Balb/c". International Journal of Molecular Medicine 37, no. 3 (2016): 716-726. https://doi.org/10.3892/ijmm.2016.2465
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