Open Access

Src promotes cutaneous wound healing by regulating MMP-2 through the ERK pathway

  • Authors:
    • Xue Wu
    • Longlong Yang
    • Zhao Zheng
    • Zhenzhen Li
    • Jihong Shi
    • Yan Li
    • Shichao Han
    • Jianxin Gao
    • Chaowu Tang
    • Linlin Su
    • Dahai Hu
  • View Affiliations

  • Published online on: January 28, 2016     https://doi.org/10.3892/ijmm.2016.2472
  • Pages: 639-648
  • Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Wound healing is a highly orchestrated, multistep process, and delayed wound healing is a significant symptomatic clinical problem. Keratinocyte migration and re-epithelialization play the most important roles in wound healing, as they determine the rate of wound healing. In our previous study, we found that Src, one of the oldest proto‑oncogenes encoding a membrane-associated, non-receptor protein tyrosine kinase, promotes keratinocyte migration. We therefore hypothesized that Src promotes wound healing through enhanced keratinocyte migration. In order to test this hypothesis, vectors for overexpressing Src and small interfering RNAs (siRNAs) for silencing of Src were used in the present study. We found that the overexpression of Src accelerated keratinocyte migration in vitro and promoted wound healing in vivo without exerting a marked effect on cell proliferation. The extracellular signal-regulated kinase (ERK) and c-Jun N-terminal kinase (JNK) signaling pathways play important roles in Src-accelerated keratinocyte migration. Further experiments demonstrated that Src induced the protein expression of matrix metalloproteinase-2 (MMP-2) and decreased the protein expression of E-cadherin. We suggest that ERK signaling is involved in the Src-mediated regulation of MMP-2 expression. The present study provided evidence that Src promotes keratinocyte migration and cutaneous wound healing, in which the regulation of MMP-2 through the ERK pathway plays an important role, and thus we also demonstrated a potential therapeutic role for Src in cutaneous wound healing.
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March-2016
Volume 37 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Wu X, Yang L, Zheng Z, Li Z, Shi J, Li Y, Han S, Gao J, Tang C, Su L, Su L, et al: Src promotes cutaneous wound healing by regulating MMP-2 through the ERK pathway. Int J Mol Med 37: 639-648, 2016.
APA
Wu, X., Yang, L., Zheng, Z., Li, Z., Shi, J., Li, Y. ... Hu, D. (2016). Src promotes cutaneous wound healing by regulating MMP-2 through the ERK pathway. International Journal of Molecular Medicine, 37, 639-648. https://doi.org/10.3892/ijmm.2016.2472
MLA
Wu, X., Yang, L., Zheng, Z., Li, Z., Shi, J., Li, Y., Han, S., Gao, J., Tang, C., Su, L., Hu, D."Src promotes cutaneous wound healing by regulating MMP-2 through the ERK pathway". International Journal of Molecular Medicine 37.3 (2016): 639-648.
Chicago
Wu, X., Yang, L., Zheng, Z., Li, Z., Shi, J., Li, Y., Han, S., Gao, J., Tang, C., Su, L., Hu, D."Src promotes cutaneous wound healing by regulating MMP-2 through the ERK pathway". International Journal of Molecular Medicine 37, no. 3 (2016): 639-648. https://doi.org/10.3892/ijmm.2016.2472