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Silencing of phosphoglucose isomerase/autocrine motility factor decreases U87 human glioblastoma cell migration

  • Authors:
    • Yang Li
    • Zhenqing Wei
    • Bin Dong
    • Zhigang Lian
    • Yinghui Xu
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 998-1004
    |
    Published online on: February 23, 2016
       https://doi.org/10.3892/ijmm.2016.2500
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Abstract

Phosphoglucose isomerase/autocrine motility factor (PGI/AMF) is secreted by tumors and influences tumor growth and metastasis. In order to investigate the effects of silencing PGI/AMF on the migration and the sphere forming abilities of human glioblastoma U87 cells, as well as on the side population cells (SPCs), PGI/AMF was silenced using siRNA. Western blot analysis and RT-qPCR were used to assess the expression of PGI/AMF, Akt and SRY (sex determining region Y)-box 2 (SOX2). Wound healing, migration and tumorsphere formation assays were performed to assess invasion and metastatic potential. The proportion of SPCs was determined using Hoechst 33342 dye and flow cytometric analysis. PGI/AMF silencing inhibited the wound healing capacity and migration ability of U87 cells by 52.6 and 80.4%, respectively, compared with the scrambled siRNA (both P<0.001). Silencing of PGI/AMF decreased the proportion of SPCs in the U87 cells by 80.9% (P<0.01). The silencing of PGI/AMF decreased the number and size of tumorspheres by 53.1 and 39.9%, respectively, compared with the scrambled siRNA (both P<0.01). The silencing of PGI/AMF decreased the levels of phosphorylated Akt (-71.9%, P<0.001) compared with the scrambled siRNA, as well as the levels of the stemness marker, SOX2 (-61.7%, P<0.01). Taken together, these findings suggest that PGI/AMF silencing decreases migration, tumorsphere formation as well as the proportion of SPCs in glioblastoma U87 cells. We suggest that the Akt pathway is involved, and our results provide a potential new target for the treatment of glioblastoma.
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Copy and paste a formatted citation
Spandidos Publications style
Li Y, Wei Z, Dong B, Lian Z and Xu Y: Silencing of phosphoglucose isomerase/autocrine motility factor decreases U87 human glioblastoma cell migration. Int J Mol Med 37: 998-1004, 2016.
APA
Li, Y., Wei, Z., Dong, B., Lian, Z., & Xu, Y. (2016). Silencing of phosphoglucose isomerase/autocrine motility factor decreases U87 human glioblastoma cell migration. International Journal of Molecular Medicine, 37, 998-1004. https://doi.org/10.3892/ijmm.2016.2500
MLA
Li, Y., Wei, Z., Dong, B., Lian, Z., Xu, Y."Silencing of phosphoglucose isomerase/autocrine motility factor decreases U87 human glioblastoma cell migration". International Journal of Molecular Medicine 37.4 (2016): 998-1004.
Chicago
Li, Y., Wei, Z., Dong, B., Lian, Z., Xu, Y."Silencing of phosphoglucose isomerase/autocrine motility factor decreases U87 human glioblastoma cell migration". International Journal of Molecular Medicine 37, no. 4 (2016): 998-1004. https://doi.org/10.3892/ijmm.2016.2500
Copy and paste a formatted citation
x
Spandidos Publications style
Li Y, Wei Z, Dong B, Lian Z and Xu Y: Silencing of phosphoglucose isomerase/autocrine motility factor decreases U87 human glioblastoma cell migration. Int J Mol Med 37: 998-1004, 2016.
APA
Li, Y., Wei, Z., Dong, B., Lian, Z., & Xu, Y. (2016). Silencing of phosphoglucose isomerase/autocrine motility factor decreases U87 human glioblastoma cell migration. International Journal of Molecular Medicine, 37, 998-1004. https://doi.org/10.3892/ijmm.2016.2500
MLA
Li, Y., Wei, Z., Dong, B., Lian, Z., Xu, Y."Silencing of phosphoglucose isomerase/autocrine motility factor decreases U87 human glioblastoma cell migration". International Journal of Molecular Medicine 37.4 (2016): 998-1004.
Chicago
Li, Y., Wei, Z., Dong, B., Lian, Z., Xu, Y."Silencing of phosphoglucose isomerase/autocrine motility factor decreases U87 human glioblastoma cell migration". International Journal of Molecular Medicine 37, no. 4 (2016): 998-1004. https://doi.org/10.3892/ijmm.2016.2500
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