Concentrated growth factor promotes Schwann cell migration partly through the integrin β1-mediated activation of the focal adhesion kinase pathway

Qin,Jie; Wang,Lin; Zheng,Ling; Zhou,Xiaoyan; Zhang,Yidi; Yang,Tingting; Zhou,Yanmin

doi:10.3892/ijmm.2016.2520

Concentrated growth factor promotes Schwann cell migration partly through the integrin β1-mediated activation of the focal adhesion kinase pathway

Authors:
- Jie Qin
- Lin Wang
- Ling Zheng
- Xiaoyan Zhou
- Yidi Zhang
- Tingting Yang
- Yanmin Zhou
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Affiliations: Department of Dental Implantology, School and Hospital of Stomatology, Jilin University, Changchun, Jilin 130021, P.R. China, Department of Very Important People, School and Hospital of Stomatology, Jilin University, Changchun, Jilin 130021, P.R. China, Department of Stomatology, Xuan Wu Hospital, Capital Medical University, Beijing 100053, P.R. China, Institute of Dental Research, Westmead Centre for Oral Health and Westmead Millennium Institute, Sydney, NSW 2145, Australia
Published online on: March 8, 2016 https://doi.org/10.3892/ijmm.2016.2520
Pages: 1363-1370

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Abstract

Nerve injury is a serious complication associated with dental implant surgery. Following nerve injury, the migration of Schwann cells (SCs) supports nerve regeneration. Concentrated growth factor (CGF) belongs to a new generation of biomaterials that contain fibrin matrix, as well as a number of growth factors that affect the migration of various types of cells, including endothelial cells and cancer cells. To the very best of our knowledge, there are no available studies to date on the promoting effect of CGF on the migration of SCs. Thus, the aim of the present study was to characterize the structure of CGF and growth factor release, examine the effects of CGF on the migration of SCs, and to examine the role of integrin β1 and the focal adhesion kinase (FAK) pathway in the CGF-induced migration of SCs. For this purpose, CGF was prepared by centrifuging rat venous blood, which produced a fiber-like matrix capable of releasing transforming growth factor-β1 (TGF-β1) over a sustained period of time (at least 13 days). The soluble component of CGF was used to produce conditioned media to treat the SC cells in culture. The results demonstrated that CGF promoted the migration of SCs and increased the expression of integrin β1. These effects appeared to involve FAK phosphorylation, which occurred downstream of integrin β1 activation. The short-interfering RNA (siRNA)-mediated downregulation of integrin β1 expression did not block the ability of CGF to promote the migration of SCs. These data suggest that CGF promotes the migration of SCs partly through the integrin β1-mediated activation of the FAK pathway.

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