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Article

Icariin regulates systemic iron metabolism by increasing hepatic hepcidin expression through Stat3 and Smad1/5/8 signaling

  • Authors:
    • Miao Zhang
    • Jing Liu
    • Wenli Guo
    • Xin Liu
    • Sijin Liu
    • Huijun Yin
  • View Affiliations / Copyright

    Affiliations: Department of Cardiovascular Diseases, Beijing Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, P.R. China, State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, P.R. China
  • Pages: 1379-1388
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    Published online on: April 1, 2016
       https://doi.org/10.3892/ijmm.2016.2545
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Abstract

Systemic iron homeostasis is strictly controlled under normal conditions to ensure a balance between the absorption, utilization, storage and recycling of iron. The hepcidin-ferroportin (FPN) axis is of critical importance in the maintenance of iron homeostasis. Hepcidin deficiency gives rise to enhanced dietary iron absorption, as well as to increased iron release from macrophages, and this in turn results in iron accumulation in the plasma and organs, and is associated with a range of tissue pathologies. Low hepcidin levels have been demonstrated in most forms of hereditary hemochromatosis (HH), as well as in β-thalassemia. Therapies that increase hepcidin concentrations may potentially play a role in the treatment of these iron overload-related diseases. To date, natural compounds have not been extensively investigated for this purpose, to the best of our knowledge. Thus, in the present study, we screened natural compounds that have the potential to regulate hepcidin expression. By performing hepcidin promoter-luciferase assay, RT-qPCR and animal experiments, we demonstrated that icariin and berberine were potent stimulators of hepcidin transcription. Mechanistic experiments indicated that icariin and berberine increased hepcidin expression by activating the signal transducer and activator of transcription 3 (Stat3) and Smad1/5/8 signaling pathways. The induction of hepcidin was confirmed in mice following icariin administration, coupled with associated changes in serum and tissue iron concentrations. In support of these findings, the icariin analogues, epimedin A, B and C, also increased hepatic hepcidin expression. However, these changes were not observed in hepcidin-deficient [Hamp1-/- or Hamp1‑knockout (KO)] mice following icariin administration, thereby verifying hepatic hepcidin as the target of icariin. Although berberine exhibited a robust capacity to promote hepcidin expression in vitro, it failed to alter hepcidin expression in mice. Taken together, the findings of the present study suggest that icariin exhibits a robust capacity to increase hepatic hepcidin expression and to modulate systemic iron homeostasis. The present study therefore highlights the significance of using natural compounds to ameliorate iron disorders through the regulation of hepcidin expression.
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View References

1 

Hentze MW, Muckenthaler MU and Andrews NC: Balancing acts: molecular control of mammalian iron metabolism. Cell. 117:285–297. 2004. View Article : Google Scholar : PubMed/NCBI

2 

Wu Y and Brosh RM Jr: DNA helicase and helicase-nuclease enzymes with a conserved iron-sulfur cluster. Nucleic Acids Res. 40:4247–4260. 2012. View Article : Google Scholar : PubMed/NCBI

3 

Brzóska K, Meczyńska S and Kruszewski M: Iron-sulfur cluster proteins: electron transfer and beyond. Acta Biochim Pol. 53:685–691. 2006.PubMed/NCBI

4 

Evstatiev R and Gasche C: Iron sensing and signalling. Gut. 61:933–952. 2012. View Article : Google Scholar

5 

Ganz T and Nemeth E: Hepcidin and disorders of iron metabolism. Annu Rev Med. 62:347–360. 2011. View Article : Google Scholar

6 

Ganz T and Nemeth E: Hepcidin and iron homeostasis. Biochim Biophys Acta. 1823:1434–1443. 2012. View Article : Google Scholar : PubMed/NCBI

7 

Camaschella C: Understanding iron homeostasis through genetic analysis of hemochromatosis and related disorders. Blood. 106:3710–3717. 2005. View Article : Google Scholar : PubMed/NCBI

8 

Ganz T: Systemic iron homeostasis. Physiol Rev. 93:1721–1741. 2013. View Article : Google Scholar : PubMed/NCBI

9 

Tanno T, Bhanu NV, Oneal PA, Goh SH, Staker P, Lee YT, Moroney JW, Reed CH, Luban NL, Wang RH, et al: High levels of GDF15 in thalassemia suppress expression of the iron regulatory protein hepcidin. Nat Med. 13:1096–1101. 2007. View Article : Google Scholar : PubMed/NCBI

10 

Tanno T, Porayette P, Sripichai O, Noh SJ, Byrnes C, Bhupatiraju A, Lee YT, Goodnough JB, Harandi O, Ganz T, et al: Identification of TWSG1 as a second novel erythroid regulator of hepcidin expression in murine and human cells. Blood. 114:181–186. 2009. View Article : Google Scholar : PubMed/NCBI

11 

Kautz L, Jung G, Valore EV, Rivella S, Nemeth E and Ganz T: Identification of erythroferrone as an erythroid regulator of iron metabolism. Nat Genet. 46:678–684. 2014. View Article : Google Scholar : PubMed/NCBI

12 

Kumar A, Ekavali, Chopra K, Mukherjee M, Pottabathini R and Dhull DK: Current knowledge and pharmacological profile of berberine: an update. Eur J Pharmacol. 761:288–297. 2015. View Article : Google Scholar : PubMed/NCBI

13 

Ghosh S, Banerjee S and Sil PC: The beneficial role of curcumin on inflammation, diabetes and neurodegenerative disease: a recent update. Food Chem Toxicol. 83:111–124. 2015. View Article : Google Scholar : PubMed/NCBI

14 

Mancuso C and Santangelo R: Ferulic acid: pharmacological and toxicological aspects. Food Chem Toxicol. 65:185–195. 2014. View Article : Google Scholar : PubMed/NCBI

15 

Ren S, Zhang H, Mu Y, Sun M and Liu P: Pharmacological effects of astragaloside IV: a literature review. J Tradit Chin Med. 33:413–416. 2013. View Article : Google Scholar : PubMed/NCBI

16 

Jiang YR and Chen KJ: Pharmacological roles of ligustrazine in cardio-/cerebrovascular systems and its progress in researches of clinical application. Zhongguo Zhong Xi Yi Jie He Za Zhi. 33:707–711. 2013.In Chinese. PubMed/NCBI

17 

Chen Y, Huang JH, Ning Y and Shen ZY: Icariin and its pharmaceutical efficacy: research progress of molecular mechanism. Zhong Xi Yi Jie He Xue Bao. 9:1179–1184. 2011.In Chinese. View Article : Google Scholar : PubMed/NCBI

18 

Sun LR, Cao X, Hou FQ, Zhu XH and Gao TM: Progressive studies of paeoniflorin. Zhongguo Zhong Yao Za Zhi. 33:2028–2032. 2008.In Chinese.

19 

Jia JM, Wang ZQ, Wu LJ and Wu YL: Advance of pharmacological study on ginsenoside Rb1. Zhongguo Zhong Yao Za Zhi. 33:1371–1377. 2008.In Chinese. PubMed/NCBI

20 

Tai MC, Tsang SY, Chang LY and Xue H: Therapeutic potential of wogonin: a naturally occurring flavonoid. CNS Drug Rev. 11:141–150. 2005. View Article : Google Scholar : PubMed/NCBI

21 

Chinese Pharmacopoeia Commission: Pharmacopoeia of the People's Republic of China China. Medical Science; Press, Beijing: 2010

22 

Zhang W, Yan ZF, Gao JH, Sun L, Huang XY, Liu Z, Yu SY, Cao CJ, Zuo LJ, Chen ZJ, et al: Role and mechanism of microglial activation in iron-induced selective and progressive dopaminergic neurodegeneration. Mol Neurobiol. 49:1153–1165. 2014. View Article : Google Scholar

23 

Liu W, Zhang S, Wang L, Qu C, Zhang C, Hong L, Yuan L, Huang Z, Wang Z, Liu S and Jiang G: CdSe quantum dot (QD)-induced morphological and functional impairments to liver in mice. PLoS One. 6:e244062011. View Article : Google Scholar : PubMed/NCBI

24 

Chen Y, Wang Z, Xu M, Wang X, Liu R, Liu Q, Zhang Z, Xia T, Zhao J, Jiang G, et al: Nanosilver incurs an adaptive shunt of energy metabolism mode to glycolysis in tumor and nontumor cells. ACS Nano. 8:5813–5825. 2014. View Article : Google Scholar : PubMed/NCBI

25 

Zhang S, Chen Y, Guo W, Yuan L, Zhang D, Xu Y, Nemeth E, Ganz T and Liu S: Disordered hepcidin-ferroportin signaling promotes breast cancer growth. Cell Signal. 26:2539–2550. 2014. View Article : Google Scholar : PubMed/NCBI

26 

Lesbordes-Brion JC, Viatte L, Bennoun M, Lou DQ, Ramey G, Houbron C, Hamard G, Kahn A and Vaulont S: Targeted disruption of the hepcidin 1 gene results in severe hemochromatosis. Blood. 108:1402–1405. 2006. View Article : Google Scholar : PubMed/NCBI

27 

Ramos E, Ruchala P, Goodnough JB, Kautz L, Preza GC, Nemeth E and Ganz T: Minihepcidins prevent iron overload in a hepcidin-deficient mouse model of severe hemochromatosis. Blood. 120:3829–3836. 2012. View Article : Google Scholar : PubMed/NCBI

28 

Liu S, Suragani RN, Han A, Zhao W, Andrews NC and Chen JJ: Deficiency of heme-regulated eIF2alpha kinase decreases hepcidin expression and splenic iron in HFE−/− mice. Haematologica. 93:753–756. 2008. View Article : Google Scholar : PubMed/NCBI

29 

Liu S, Suragani RN, Wang F, Han A, Zhao W, Andrews NC and Chen JJ: The function of heme-regulated eIF2alpha kinase in murine iron homeostasis and macrophage maturation. J Clin Invest. 117:3296–3305. 2007. View Article : Google Scholar : PubMed/NCBI

30 

Park CH, Valore EV, Waring AJ and Ganz T: Hepcidin, a urinary antimicrobial peptide synthesized in the liver. J Biol Chem. 276:7806–7810. 2001. View Article : Google Scholar

31 

Hou Y, Zhang S, Wang L, Li J, Qu G, He J, Rong H, Ji H and Liu S: Estrogen regulates iron homeostasis through governing hepatic hepcidin expression via an estrogen response element. Gene. 511:398–403. 2012. View Article : Google Scholar : PubMed/NCBI

32 

Nemeth E, Rivera S, Gabayan V, Keller C, Taudorf S, Pedersen BK and Ganz T: IL-6 mediates hypoferremia of inflammation by inducing the synthesis of the iron regulatory hormone hepcidin. J Clin Invest. 113:1271–1276. 2004. View Article : Google Scholar : PubMed/NCBI

33 

Wrighting DM and Andrews NC: Interleukin-6 induces hepcidin expression through STAT3. Blood. 108:3204–3209. 2006. View Article : Google Scholar : PubMed/NCBI

34 

Pigeon C, Ilyin G, Courselaud B, Leroyer P, Turlin B, Brissot P and Loréal O: A new mouse liver-specific gene, encoding a protein homologous to human antimicrobial peptide hepcidin, is overexpressed during iron overload. J Biol Chem. 276:7811–7819. 2001. View Article : Google Scholar

35 

Poli M, Asperti M, Naggi A, Campostrini N, Girelli D, Corbella M, Benzi M, Besson-Fournier C, Coppin H, Maccarinelli F, et al: Glycol-split nonanticoagulant heparins are inhibitors of hepcidin expression in vitro and in vivo. Blood. 123:1564–1573. 2014. View Article : Google Scholar : PubMed/NCBI

36 

Lee P, Peng H, Gelbart T and Beutler E: The IL-6- and lipopolysaccharide-induced transcription of hepcidin in HFE-, transferrin receptor 2-, and beta 2-microglobulin-deficient hepatocytes. Proc Natl Acad Sci USA. 101:9263–9265. 2004. View Article : Google Scholar : PubMed/NCBI

37 

Adams PC and Barton JC: How I treat hemochromatosis. Blood. 116:317–325. 2010. View Article : Google Scholar : PubMed/NCBI

38 

Pennell DJ, Porter JB, Cappellini MD, El-Beshlawy A, Chan LL, Aydinok Y, Elalfy MS, Sutcharitchan P, Li CK, Ibrahim H, et al: Efficacy of deferasirox in reducing and preventing cardiac iron overload in beta-thalassemia. Blood. 115:2364–2371. 2010. View Article : Google Scholar

39 

Zhen AW, Nguyen NH, Gibert Y, Motola S, Buckett P, Wessling-Resnick M, Fraenkel E and Fraenkel PG: The small molecule, genistein, increases hepcidin expression in human hepatocytes. Hepatology. 58:1315–1325. 2013. View Article : Google Scholar : PubMed/NCBI

40 

Gaun V, Patchen B, Volovetz J, Zhen AW, Andreev A, Pollastri MP and Fraenkel PG: A chemical screen identifies small molecules that regulate hepcidin expression. Blood Cells Mol Dis. 53:231–240. 2014. View Article : Google Scholar : PubMed/NCBI

41 

Kennedy DO and Wightman EL: Herbal extracts and phytochemicals: plant secondary metabolites and the enhancement of human brain function. Adv Nutr. 2:32–50. 2011. View Article : Google Scholar :

42 

Ziegler G, Ploch M, Miettinen-Baumann A and Collet W: Efficacy and tolerability of valerian extract LI 156 compared with oxazepam in the treatment of non-organic insomnia - a randomized, double-blind, comparative clinical study. Eur J Med Res. 7:480–486. 2002.

43 

Guan Y, An P, Zhang Z, Zhang F, Yu Y, Wu Q, Shi Y, Guo X, Tao Y and Wang F: Screening identifies the Chinese medicinal plant Caulis Spatholobi as an effective HAMP expression inhibitor. J Nutr. 143:1061–1066. 2013. View Article : Google Scholar : PubMed/NCBI

44 

Bartnikas TB and Fleming MD: A tincture of hepcidin cures all: the potential for hepcidin therapeutics. J Clin Invest. 120:4187–4190. 2010. View Article : Google Scholar : PubMed/NCBI

45 

Ma H, He X, Yang Y, Li M, Hao D and Jia Z: The genus Epimedium: an ethnopharmacological and phytochemical review. J Ethnopharmacol. 134:519–541. 2011. View Article : Google Scholar : PubMed/NCBI

46 

Xu CQ, Liu BJ, Wu JF, Xu YC, Duan XH, Cao YX and Dong JC: Icariin attenuates LPS-induced acute inflammatory responses: involvement of PI3K/Akt and NF-kappaB signaling pathway. Eur J Pharmacol. 642:146–153. 2010. View Article : Google Scholar : PubMed/NCBI

47 

Li WW, Gao XM, Wang XM, Guo H and Zhang BL: Icariin inhibits hydrogen peroxide-induced toxicity through inhibition of phosphorylation of JNK/p38 MAPK and p53 activity. Mutat Res. 708:1–10. 2011. View Article : Google Scholar : PubMed/NCBI

48 

Song YH, Cai H, Gu N, Qian CF, Cao SP and Zhao ZM: Icariin attenuates cardiac remodelling through down-regulating myocardial apoptosis and matrix metalloproteinase activity in rats with congestive heart failure. J Pharm Pharmacol. 63:541–549. 2011. View Article : Google Scholar : PubMed/NCBI

49 

Chung BH, Kim JD, Kim CK, Kim JH, Won MH, Lee HS, Dong MS, Ha KS, Kwon YG and Kim YM: Icariin stimulates angiogenesis by activating the MEK/ERK- and PI3K/Akt/eNOS-dependent signal pathways in human endothelial cells. Biochem Biophys Res Commun. 376:404–408. 2008. View Article : Google Scholar : PubMed/NCBI

50 

Chen Y, Wang J, Jia X, Tan X and Hu M: Role of intestinal hydrolase in the absorption of prenylated flavonoids present in Yinyanghuo. Molecules. 16:1336–1348. 2011. View Article : Google Scholar : PubMed/NCBI

51 

Chen Y, Zhao YH, Jia XB and Hu M: Intestinal absorption mechanisms of prenylated flavonoids present in the heat-processed Epimedium koreanum Nakai (Yin Yanghuo). Pharm Res. 25:2190–2199. 2008. View Article : Google Scholar : PubMed/NCBI

52 

Liu M, Liu H, Lu X, Li C, Xiong Z and Li F: Simultaneous determination of icariin, icariside II and osthole in rat plasma after oral administration of the extract of Gushudan (a Chinese compound formulation) by LC-MS/MS. J Chromatogr B Analyt Technol Biomed Life Sci. 860:113–120. 2007. View Article : Google Scholar : PubMed/NCBI

53 

Xu W, Sui ZG and Zhong W: Pharmacokinetics of icariin and its two metabolites in rats. The Third China Pharmacist Assembly. 1–5. 2011.

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Copy and paste a formatted citation
Spandidos Publications style
Zhang M, Liu J, Guo W, Liu X, Liu S and Yin H: Icariin regulates systemic iron metabolism by increasing hepatic hepcidin expression through Stat3 and Smad1/5/8 signaling. Int J Mol Med 37: 1379-1388, 2016.
APA
Zhang, M., Liu, J., Guo, W., Liu, X., Liu, S., & Yin, H. (2016). Icariin regulates systemic iron metabolism by increasing hepatic hepcidin expression through Stat3 and Smad1/5/8 signaling. International Journal of Molecular Medicine, 37, 1379-1388. https://doi.org/10.3892/ijmm.2016.2545
MLA
Zhang, M., Liu, J., Guo, W., Liu, X., Liu, S., Yin, H."Icariin regulates systemic iron metabolism by increasing hepatic hepcidin expression through Stat3 and Smad1/5/8 signaling". International Journal of Molecular Medicine 37.5 (2016): 1379-1388.
Chicago
Zhang, M., Liu, J., Guo, W., Liu, X., Liu, S., Yin, H."Icariin regulates systemic iron metabolism by increasing hepatic hepcidin expression through Stat3 and Smad1/5/8 signaling". International Journal of Molecular Medicine 37, no. 5 (2016): 1379-1388. https://doi.org/10.3892/ijmm.2016.2545
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang M, Liu J, Guo W, Liu X, Liu S and Yin H: Icariin regulates systemic iron metabolism by increasing hepatic hepcidin expression through Stat3 and Smad1/5/8 signaling. Int J Mol Med 37: 1379-1388, 2016.
APA
Zhang, M., Liu, J., Guo, W., Liu, X., Liu, S., & Yin, H. (2016). Icariin regulates systemic iron metabolism by increasing hepatic hepcidin expression through Stat3 and Smad1/5/8 signaling. International Journal of Molecular Medicine, 37, 1379-1388. https://doi.org/10.3892/ijmm.2016.2545
MLA
Zhang, M., Liu, J., Guo, W., Liu, X., Liu, S., Yin, H."Icariin regulates systemic iron metabolism by increasing hepatic hepcidin expression through Stat3 and Smad1/5/8 signaling". International Journal of Molecular Medicine 37.5 (2016): 1379-1388.
Chicago
Zhang, M., Liu, J., Guo, W., Liu, X., Liu, S., Yin, H."Icariin regulates systemic iron metabolism by increasing hepatic hepcidin expression through Stat3 and Smad1/5/8 signaling". International Journal of Molecular Medicine 37, no. 5 (2016): 1379-1388. https://doi.org/10.3892/ijmm.2016.2545
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