Anti-obesity effects of boiled tuna extract in mice with obesity induced by a high-fat diet

  • Authors:
    • Youngmin Kim
    • Mi-Jin Kwon
    • Jeong-Wook Choi
    • Min-Kyeong Lee
    • Chorong Kim
    • Jaehun Jung
    • Heny Aprianita
    • Heesop Nam
    • Taek-Jeong Nam
  • View Affiliations

  • Published online on: August 1, 2016     https://doi.org/10.3892/ijmm.2016.2695
  • Pages: 1281-1288
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Abstract

The aim of this study was to examine the anti-obesity effects of boiled tuna extract in C57BL/6N mice with obesity induced by a high-fat diet (HFD). We determined the anti-obesity effects of boiled tuna extract (100, 200, or 400 mg/kg) on the progression of HFD-induced obesity for 10 weeks. The mice were divided into 5 groups as follows: the normal diet (ND) group (n=10); the HFD group (n=10); the mice fed HFD and 100 mg/kg boiled tuna extract group (n=10); those fed a HFD and 200 mg/kg boiled tuna extract group (n=10); and those fed a HFD and 400 mg/kg boiled tuna extract group (n=10). Changes in body weight, fat content, serum lipid levels and lipogenic enzyme levels were measured. The consumption of boiled tuna extract lowered epididymal tissue weight and exerted anti-obesity effects, as reflected by the serum glucose, triglyceride (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL‑C), low-density lipoprotein cholesterol (LDL-C), insulin and leptin levels. In addition, we demonstrated changes in liver adipogenic- and lipogenic-related protein expression by western blot analysis. Boiled tuna extract downregulated the levels of the CCAAT/enhancer-binding protein α, β and δ (C/EBPα, β, δ), and peroxisome proliferator-activated receptor-γ (PPAR-γ) adipocyte marker genes. Boiled tuna extract also attenuated adipogenic and lipogenic gene expression, namely the levels of fatty acid synthase (FAS), lipoprotein lipase (LPL), acetyl-CoA carboxylase (ACC), glucose transporter type 4 (Glut4) and phosphorylated adenosine monophosphate-activated protein kinase α and β (AMPKα, β) in a dose-dependent manner. Moreover, the consumption of boiled tuna extract restored the levels of superoxide dismutase (SOD), catalase (CAT), glutamic oxaloacetic transaminase (GOT), glutamic-pyruvate transaminase (GPT), aspartate transaminase (AST) and alanine transaminase (ALT) to those of the control group. These results suggest that boiled tuna extract attenuates the progression of obesity by stimulating fatty acid oxidation through the upregulation of AMPK genes, as well as by inhibiting the synthesis of adipogenic and lipogenic enzymes. These characteristics of boiled tuna extract highlight its potential anti-obesity effects.
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October-2016
Volume 38 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Kim Y, Kwon M, Choi J, Lee M, Kim C, Jung J, Aprianita H, Nam H and Nam T: Anti-obesity effects of boiled tuna extract in mice with obesity induced by a high-fat diet. Int J Mol Med 38: 1281-1288, 2016.
APA
Kim, Y., Kwon, M., Choi, J., Lee, M., Kim, C., Jung, J. ... Nam, T. (2016). Anti-obesity effects of boiled tuna extract in mice with obesity induced by a high-fat diet. International Journal of Molecular Medicine, 38, 1281-1288. https://doi.org/10.3892/ijmm.2016.2695
MLA
Kim, Y., Kwon, M., Choi, J., Lee, M., Kim, C., Jung, J., Aprianita, H., Nam, H., Nam, T."Anti-obesity effects of boiled tuna extract in mice with obesity induced by a high-fat diet". International Journal of Molecular Medicine 38.4 (2016): 1281-1288.
Chicago
Kim, Y., Kwon, M., Choi, J., Lee, M., Kim, C., Jung, J., Aprianita, H., Nam, H., Nam, T."Anti-obesity effects of boiled tuna extract in mice with obesity induced by a high-fat diet". International Journal of Molecular Medicine 38, no. 4 (2016): 1281-1288. https://doi.org/10.3892/ijmm.2016.2695